{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:09:24Z","timestamp":1792112964216,"version":"4.4.0"},"reference-count":21,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The frequency of dogs becoming ill or dying from accidental exposure to cyanotoxins, produced by cyanobacteria, is increasing throughout the United States. In January and February of 2021, two dogs died and five dogs became ill after swimming in Lake Travis, central Texas, USA; one deceased dog (C1) was subjected to pathological testing. Algal materials, sediment samples, zebra mussel viscera, periphyton from shells, as well as fluids and tissues from the digestive tract of C1 were investigated for the following cyanotoxins: anatoxin-a, homoanatoxin-a, dihydroanatoxin-a (dhATX), cylindrospermopsin, saxitoxin, and microcystins. Necropsy results of C1 indicated neurotoxicosis with significant levels of dhATX in the duodenum tissues (10.51 ng\/g dry weight (DW)), jejunum tissue (6.076 ng\/g DW), and stomach contents (974.88 ng\/g DW). Algae collected near the site of C1\u2019s death contained levels of dhATX, ranging from 13 to 33 \u00b5g\/g. By comparison, dhATX was detected at much lower concentrations in sediment samples (310.23 ng\/g DW) and the periphyton on zebra mussel shells (38.45 ng\/g DW). While dhATX was suspected in the deaths of canines from an event in Texas in 2019, this is the first report linking dhATX neurotoxicosis through pathological findings in Texas and potentially in the United States.<\/jats:p>","DOI":"10.3390\/toxins15080485","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:24:24Z","timestamp":1690881864000},"page":"485","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["First Confirmed Case of Canine Mortality Due to Dihydroanatoxin-a in Central Texas, USA"],"prefix":"10.3390","volume":"15","author":[{"given":"Anthea","family":"Fredrickson","sequence":"first","affiliation":[{"name":"Lower Colorado River Authority, 3700 Lake Austin Blvd, Austin, TX 78703, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Richter","sequence":"additional","affiliation":[{"name":"Lower Colorado River Authority, 3700 Lake Austin Blvd, Austin, TX 78703, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A.","family":"Perri","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Institute of Environment, Florida International University, 3000 NE 151st Street, North Miami, FL 33181, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Schonna R.","family":"Manning","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Institute of Environment, Florida International University, 3000 NE 151st Street, North Miami, FL 33181, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1007\/s00204-016-1913-6","article-title":"Cyanotoxins: Producing Organisms, Occurrence, Toxicity, Mechanism of Action and Human Health Toxicological Risk Evaluation","volume":"91","author":"Buratti","year":"2017","journal-title":"Arch. Toxicol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106344","DOI":"10.1016\/j.dib.2020.106344","article-title":"Bloom Announcement: First Reports of Dog Mortalities Associated with Neurotoxic Filamentous Cyanobacterial Mats at Recreational Sites in Lady Bird Lake, Austin, Texas","volume":"33","author":"Manning","year":"2020","journal-title":"Data Brief"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.3390\/toxins5091597","article-title":"Canine Cyanotoxin Poisonings in the United States (1920s\u20132012): Review of Suspected and Confirmed Cases from Three Data Sources","volume":"5","author":"Backer","year":"2013","journal-title":"Toxins"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Fastner, J., Beulker, C., Geiser, B., Hoffmann, A., Kr\u00f6ger, R., Teske, K., Hoppe, J., Mundhenk, L., Neurath, H., and Sagebiel, D. (2018). Fatal Neurotoxicosis in Dogs Associated with Tychoplanktic, Anatoxin-a Producing Tychonema sp. in Mesotrophic Lake Tegel, Berlin. Toxins, 10.","DOI":"10.3390\/toxins10020060"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Bauer, F., Fastner, J., Bartha-Dima, B., Breuer, W., Falkenau, A., Mayer, C., and Raeder, U. (2020). Mass Occurrence of Anatoxin-a- and Dihydroanatoxin-a-Producing Tychonema Sp. in Mesotrophic Reservoir Mandichosee (River Lech, Germany) as a Cause of Neurotoxicosis in Dogs. Toxins, 12.","DOI":"10.3390\/toxins12110726"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.toxicon.2007.03.025","article-title":"First Report of Homoanatoxin-a and Associated Dog Neurotoxicosis in New Zealand","volume":"50","author":"Wood","year":"2007","journal-title":"Toxicon"},{"key":"ref_7","unstructured":"Mayer, C.M., Burlakova, L.E., Ekl\u00f6v, P., Fitzgerald, D., Karatayev, A.Y., Ludsin, S.A., Millard, S., Mills, E.L., Ostapenya, A.P., and Rudstam, L.G. (2012). Quagga and Zebra Mussels: Biology Impacts and Control, CRC Press. [2nd ed.]."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5464","DOI":"10.1016\/j.watres.2013.06.042","article-title":"A Review of Current Knowledge on Toxic Benthic Freshwater Cyanobacteria\u2014Ecology, Toxin Production and Risk Management","volume":"47","author":"Quiblier","year":"2013","journal-title":"Water Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.toxicon.2012.04.335","article-title":"First Report of (Homo)Anatoxin-a and Dog Neurotoxicosis after Ingestion of Benthic Cyanobacteria in The Netherlands","volume":"60","author":"Faassen","year":"2012","journal-title":"Toxicon"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"127937","DOI":"10.1016\/j.chemosphere.2020.127937","article-title":"Acute Toxicity of Dihydroanatoxin-a from Microcoleus Autumnalis in Comparison to Anatoxin-a","volume":"263","author":"Puddick","year":"2021","journal-title":"Chemosphere"},{"key":"ref_11","unstructured":"World Health Organization (2021). WHO Guidelines on Recreational Water Quality: Volume 1: Coastal and Fresh Waters, World Health Organization."},{"key":"ref_12","unstructured":"Perri, K.A., Manning, S.R., Richter, A., Petri, C., and Fredrickson, A. (2023). Water Quality and Biochemical Analysis Associated with a Canine Death Linked to Neurotoxic Cyanobacterial Metaphyton in Lake Travis, Austin, Texas, Mendelay Data, v1."},{"key":"ref_13","unstructured":"Milne, J.R., and Watts, L. (2023, March 24). Toxic Benthic Cyanobacteria Proliferations in Wellington\u2019s Rivers in 2005\/06, Available online: https:\/\/www.gw.govt.nz\/assets\/Documents\/2009\/07\/4097_Toxicbenthiccyan_s8141.pdf."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Turner, A.D., Turner, F.R.I., White, M., Hartnell, D., Crompton, C.G., Bates, N., Egginton, J., Branscombe, L., Lewis, A.M., and Maskrey, B.H. (2022). Confirmation Using Triple Quadrupole and High-Resolution Mass Spectrometry of a Fatal Canine Neurotoxicosis Following Exposure to Anatoxins at an Inland Reservoir. Toxins, 14.","DOI":"10.3390\/toxins14110804"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1002\/lol2.10316","article-title":"Blooms Also like It Cold","volume":"8","author":"Reinl","year":"2023","journal-title":"Limnol. Oceanogr. Lett."},{"key":"ref_16","unstructured":"Texas Parks and Wildlife (2023, March 23). Invasive Zebra Mussels Discovered in Lake Travis, Available online: https:\/\/tpwd.texas.gov\/newsmedia\/releases\/?req=20170627a."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1093\/biosci\/biab049","article-title":"Blue Waters, Green Bottoms: Benthic Filamentous Algal Blooms Are an Emerging Threat to Clear Lakes Worldwide","volume":"71","author":"Vadeboncoeur","year":"2021","journal-title":"BioScience"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1139\/f07-181","article-title":"Invasive Zebra Mussels (Dreissena Polymorpha) Increase Cyanobacterial Toxin Concentrations in Low-Nutrient Lakes","volume":"65","author":"Knoll","year":"2008","journal-title":"Can. J. Fish. Aquat. Sci."},{"key":"ref_19","unstructured":"Texas Commission on Environmental Quality (2023, March 23). Surface Water Quality Monitoring Procedures, Volume 1: Physical and Chemical Monitoring Methods, Available online: https:\/\/www.tceq.texas.gov\/downloads\/publications\/rg\/chapter-5-rg-415.pdf."},{"key":"ref_20","unstructured":"R Core Team (2020). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1080\/01621459.1979.10481038","article-title":"Robust Locally Weighted Regression and Smoothing Scatterplots","volume":"74","author":"Cleveland","year":"1979","journal-title":"J. Am. Stat. Assoc."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/15\/8\/485\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:23:45Z","timestamp":1760127825000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/15\/8\/485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,1]]},"references-count":21,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["toxins15080485"],"URL":"https:\/\/doi.org\/10.3390\/toxins15080485","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,1]]}}}