{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T10:06:23Z","timestamp":1783850783475,"version":"3.55.0"},"reference-count":70,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T00:00:00Z","timestamp":1691539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"CONACYT Mexico","doi-asserted-by":"publisher","award":["303045"],"award-info":[{"award-number":["303045"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"CONACYT Mexico","doi-asserted-by":"publisher","award":["DGAPA-IN200323"],"award-info":[{"award-number":["DGAPA-IN200323"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"CONACYT Mexico","doi-asserted-by":"publisher","award":["UCOL-CA 117"],"award-info":[{"award-number":["UCOL-CA 117"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","award":["303045"],"award-info":[{"award-number":["303045"]}]},{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","award":["DGAPA-IN200323"],"award-info":[{"award-number":["DGAPA-IN200323"]}]},{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","award":["UCOL-CA 117"],"award-info":[{"award-number":["UCOL-CA 117"]}]},{"name":"Fortalecimiento de Cuerpos Acad\u00e9micos PRODEP","award":["303045"],"award-info":[{"award-number":["303045"]}]},{"name":"Fortalecimiento de Cuerpos Acad\u00e9micos PRODEP","award":["DGAPA-IN200323"],"award-info":[{"award-number":["DGAPA-IN200323"]}]},{"name":"Fortalecimiento de Cuerpos Acad\u00e9micos PRODEP","award":["UCOL-CA 117"],"award-info":[{"award-number":["UCOL-CA 117"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Centruroides possanii is a recently discovered species of \u201cstriped scorpion\u201d found in Mexico. Certain species of Centruroides are known to be toxic to mammals, leading to numerous cases of human intoxications in the country. Venom components are thought to possess therapeutic potential and\/or biotechnological applications. Hence, obtaining and analyzing the secretory gland transcriptome and venom proteome of C. possanii is relevant, and that is what is described in this communication. Since this is a newly described species, first, its LD50 to mice was determined and estimated to be 659 ng\/g mouse weight. Using RNA extracted from this species and preparing their corresponding cDNA fragments, a transcriptome analysis was obtained on a Genome Analyzer (Illumina) using the 76-base pair-end sequencing protocol. Via high-throughput sequencing, 19,158,736 reads were obtained and ensembled in 835,204 sequences. Of them, 28,399 transcripts were annotated with Pfam. A total of 244 complete transcripts were identified in the transcriptome of C. possanii. Of these, 109 sequences showed identity to toxins that act on ion channels, 47 enzymes, 17 protease inhibitors (PINs), 11 defense peptides (HDPs), and 60 in other components. In addition, a sample of the soluble venom obtained from this scorpion was analyzed using an Orbitrap Velos apparatus, which allowed for identification by liquid chromatography followed by mass spectrometry (LC-MS\/MS) of 70 peptides and proteins: 23 toxins, 27 enzymes, 6 PINs, 3 HDPs, and 11 other components. Until now, this work has the highest number of scorpion venom components identified through omics technologies. The main novel findings described here were analyzed in comparison with the known data from the literature, and this process permitted some new insights in this field.<\/jats:p>","DOI":"10.3390\/toxins15080498","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T10:21:50Z","timestamp":1691576510000},"page":"498","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Unveiling the Protein Components of the Secretory-Venom Gland and Venom of the Scorpion Centruroides possanii (Buthidae) through Omic Technologies"],"prefix":"10.3390","volume":"15","author":[{"given":"Patricia Elizabeth","family":"Garc\u00eda-Villalvazo","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad de Colima, Colima 28400, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana Mar\u00eda","family":"Jim\u00e9nez-Vargas","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad de Colima, Colima 28400, Mexico"},{"name":"Consejo Nacional de Humanidades, Ciencia y Tecnolog\u00eda (CONAHCYT), Mexico City 03940, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela Jareth","family":"Lino-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Referencia de Control Biol\u00f3gico, Direcci\u00f3n General de Sanidad Vegetal SENASICASADER, Colima 28110, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika Patricia","family":"Meneses","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca 62210, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel de Jes\u00fas","family":"Berm\u00fadez-Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Agr\u00edcolas y Pecuarias (INIFAP), Instituto Nacional de Investigaciones Forestales, Colima 28110, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9506-188X","authenticated-orcid":false,"given":"Carlos Eduardo","family":"Barajas-Saucedo","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad de Colima, Colima 28400, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9848-862X","authenticated-orcid":false,"given":"Iv\u00e1n","family":"Delgado Enciso","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Universidad de Colima, Colima 28040, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6357-2661","authenticated-orcid":false,"given":"Lourival Domingos","family":"Possani","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca 62210, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8474-1495","authenticated-orcid":false,"given":"Laura Leticia","family":"Valdez-Velazquez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad de Colima, Colima 28400, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,9]]},"reference":[{"key":"ref_1","unstructured":"Louren\u00e7o, W.R. 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