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These assays require the use of large numbers of mice that manifest severe signs associated with pain and distress and long periods of suffering. Despite this, many animals make a full recovery; therefore, the observation of clinical signs as a predictor of animal death is highly subjective and could affect the accuracy of the results. The use of a more objective parameter such as body temperature measurement could help establish a humane endpoint that would contribute to significantly reducing the suffering of large numbers of animals. We determined the temperature drop in BALB\/c mice exposed to the mixtures of Bothrops asper or Lachesis stenophrys venom and a polyvalent antivenom by using an infrared thermometer. Our data show that, based on the temperature change from baseline, it is possible to predict which animals will survive during the first 3 h after inoculation. The data provided in this study may contribute to future reductions in animal suffering, in concordance with general trends in the use of laboratory animals for the quality control of biologicals.<\/jats:p>","DOI":"10.3390\/toxins15090525","type":"journal-article","created":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T05:46:47Z","timestamp":1693201607000},"page":"525","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Body Temperature Drop as a Humane Endpoint in Snake Venom-Lethality Neutralization Tests"],"prefix":"10.3390","volume":"15","author":[{"given":"Rosa","family":"De Jesus","sequence":"first","affiliation":[{"name":"Bioterio, Instituto de Investigaciones Cient\u00edficas y Servicios de Alta Tecnolog\u00eda (INDICASAT AIP), City of Knowledge, Panama City 0843-01103, Panama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2439-4289","authenticated-orcid":false,"given":"Adam","family":"Tratner","sequence":"additional","affiliation":[{"name":"Florida State University, Republic of Panama Campus, City of Knowledge, Panama City 0843-01103, Panama"},{"name":"Centro de Neurociencias, INDICASAT AIP, City of Knowledge, Panama City 0843-01103, Panama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alanna","family":"Madrid","sequence":"additional","affiliation":[{"name":"Bioterio, Instituto de Investigaciones Cient\u00edficas y Servicios de Alta Tecnolog\u00eda (INDICASAT AIP), City of Knowledge, Panama City 0843-01103, Panama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7426-5405","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Rivera-Mondrag\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto Especializado de An\u00e1lisis (IEA), Universidad de Panam\u00e1, Panama City P.O. 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