{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:13:02Z","timestamp":1785337982642,"version":"3.55.0"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agriculture &amp; Agri-Food Canada","award":["CAP-ASP-110"],"award-info":[{"award-number":["CAP-ASP-110"]}]},{"name":"Agriculture &amp; Agri-Food Canada","award":["SCAP-ASP-026"],"award-info":[{"award-number":["SCAP-ASP-026"]}]},{"name":"Agriculture &amp; Agri-Food Canada","award":["CRDA J-002398"],"award-info":[{"award-number":["CRDA J-002398"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Zearalenone and radicicol are resorcylic acid lactones produced by numerous plant pathogenic fungi. Zearalenone is a non-steroidal estrogen mimic that can cause serious reproductive issues in livestock that consume contaminated feed. Radicicol is a potent inhibitor of the molecular chaperone Hsp90, which, in plants, has an important role in coordinating the host\u2019s immune response during infection. Here, we describe the identification and characterization of a soil-borne strain of the Gram-positive bacterium Aeromicrobium sp. capable of hydrolyzing the macrolide ring of resorcylic acid lactones, including zearalenone and radicicol. Proteomic analysis of biochemically enriched fractions from the isolated and cultured bacterium identified an \u03b1\/\u03b2-hydrolase responsible for this activity. A recombinantly expressed and purified form of the hydrolase (termed RALH) was active against both zearalenone and radicicol. Interpretation of high-resolution mass spectrometry and NMR data confirmed the structures of the enzymatic products as the previously reported non-toxic metabolite hydrolyzed zearalenone and hydrolyzed radicicol. Hydrolyzed radicicol was demonstrated to no longer inhibit the ATPase activity of the Saccharomyces cerevisiae Hsp90 homolog in vitro. Enzymatic degradation of resorcylic acid lactones will enable insight into their biological functions.<\/jats:p>","DOI":"10.3390\/toxins16090404","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T03:34:20Z","timestamp":1726716860000},"page":"404","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Enzymatic Hydrolysis of Resorcylic Acid Lactones by an Aeromicrobium sp."],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2533-9310","authenticated-orcid":false,"given":"Shawn J.","family":"Hoogstra","sequence":"first","affiliation":[{"name":"London Research and Development Center, Agriculture and Agri-Food Canada, London, ON N5V 4T3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2303-2438","authenticated-orcid":false,"given":"Kyle N.","family":"Hendricks","sequence":"additional","affiliation":[{"name":"London Research and Development Center, Agriculture and Agri-Food Canada, London, ON N5V 4T3, Canada"},{"name":"Department of Biology, Western University, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9808-924X","authenticated-orcid":false,"given":"David R.","family":"McMullin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin B.","family":"Renaud","sequence":"additional","affiliation":[{"name":"London Research and Development Center, Agriculture and Agri-Food Canada, London, ON N5V 4T3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhi","family":"Bora","sequence":"additional","affiliation":[{"name":"London Research and Development Center, Agriculture and Agri-Food Canada, London, ON N5V 4T3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0977-1656","authenticated-orcid":false,"given":"Mark W.","family":"Sumarah","sequence":"additional","affiliation":[{"name":"London Research and Development Center, Agriculture and Agri-Food Canada, London, ON N5V 4T3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Garnham","sequence":"additional","affiliation":[{"name":"London Research and Development Center, Agriculture and Agri-Food Canada, London, ON N5V 4T3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Winssinger, N., and Barluenga, S. 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