{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T18:19:31Z","timestamp":1788459571164,"version":"build-2803163510"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation (FAPESP)","award":["2020\/08863-1"],"award-info":[{"award-number":["2020\/08863-1"]}]},{"name":"Funda\u00e7\u00e3o Butantan","award":["2020\/08863-1"],"award-info":[{"award-number":["2020\/08863-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Jararhagin-C (JarC) is a protein from the venom of Bothrops jararaca consisting of disintegrin-like and cysteine-rich domains. JarC shows a modulating effect on angiogenesis and remodeling of extracellular matrix constituents, improving wound healing in a mouse experimental model. JarC is purified from crude venom, and the yield is less than 1%. The aim of this work was to obtain the recombinant form of JarC and to test its biological activity. For this purpose, the bicistronic vector pSUMOUlp1 was used. This vector allowed the expression of the recombinant toxin JarC (rJarC) in fusion with the small ubiquitin-related modifier (SUMO) as well as the SUMO protease Ulp1. After expression, this protease was able to efficiently remove SUMO from rJarC inside the bacteria. rJarC free from SUMO was purified at the expected molecular mass and recognized by polyclonal anti-jararhagin antibodies. In terms of biological activity, both the native and recombinant forms showed no toxicity to the HUVEC cell line CRL1730 and were effective in modulating cell migration activity in the experimental in vitro model. These results demonstrate the successful production of rJarC and the preservation of its biological activity, which may facilitate further investigations into the therapeutic potential of this snake venom-derived protein.<\/jats:p>","DOI":"10.3390\/toxins16120524","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T10:52:30Z","timestamp":1733223150000},"page":"524","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bicistronic Vector Expression of Recombinant Jararhagin-C and Its Effects on Endothelial Cells"],"prefix":"10.3390","volume":"16","author":[{"given":"Karla Fernanda","family":"Ferraz","sequence":"first","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7388-5036","authenticated-orcid":false,"given":"Lhiri Hanna","family":"De Lucca Caetano","sequence":"additional","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele Pereira","family":"Orefice","sequence":"additional","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7955-4724","authenticated-orcid":false,"given":"Paula Andreia Lucas","family":"Calabria","sequence":"additional","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maisa Splendore","family":"Della-Casa","sequence":"additional","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4426-0731","authenticated-orcid":false,"given":"Luciana Aparecida","family":"Freitas-de-Sousa","sequence":"additional","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4735-5162","authenticated-orcid":false,"given":"Emidio","family":"Beraldo-Neto","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8876-8262","authenticated-orcid":false,"given":"Sabri Saeed","family":"Sanabani","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of S\u00e3o Paulo, Sao Paulo 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4292-7827","authenticated-orcid":false,"given":"Geraldo Santana","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia Bianca","family":"Clissa","sequence":"additional","affiliation":[{"name":"Immunopathology Laboratory, Butantan Institute, S\u00e3o Paulo 05585-090, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.toxicon.2005.02.024","article-title":"Snake venom disintegrins: Evolution of structure and function","volume":"45","author":"Calvete","year":"2005","journal-title":"Toxicon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1093\/molbev\/msn179","article-title":"Evolution of snake venom disintegrins by positive Darwinian selection","volume":"25","author":"Comas","year":"2008","journal-title":"Mol. 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