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Recently, biochar (BC), derived from rice straw, has emerged as a potent adsorbent for eliminating hazardous contaminants from water. To assess the joint hepatotoxic effects of environmentally relevant concentrations of MC-LR and BC on fish, male adult zebrafish (Danio rerio) were sub-chronically co-exposed to varying concentrations of MC-LR (0, 1, 5, and 25 \u03bcg\/L) and BC (0 and 100 \u03bcg\/L) in a fully factorial experiment. After 30 days exposure, our findings suggested that the existence of BC significantly decreased MC-LR bioavailability in liver. Furthermore, histopathological analysis revealed that BC mitigated MC-LR-induced hepatic lesions, which were characterized by mild damage, such as vacuolization, pyknotic nuclei, and swollen mitochondria. Compared to the groups exposed solely to MC-LR, decreased malondialdehyde (MDA) and increased catalase (CAT) and superoxide dismutase (SOD) were noticed in the mixture groups. Concurrently, significant changes in the mRNA expression levels of Nrf2 pathway genes (cat, sod1, gstr, keap1a, nrf2a, and gclc) further proved that BC reduces the oxidative damage induced by MC-LR. These findings demonstrate that BC decreases MC-LR bioavailability in the liver, thereby alleviating MC-LR-induced hepatotoxicity through the Nrf2 signaling pathway in zebrafish. Our results also imply that BC could serve as a potentially environmentally friendly material for mitigating the detrimental effects of MC-LR on fish.<\/jats:p>","DOI":"10.3390\/toxins16120549","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T11:17:22Z","timestamp":1734520642000},"page":"549","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Rice Straw-Derived Biochar Mitigates Microcystin-LR-Induced Hepatic Histopathological Injury and Oxidative Damage in Male Zebrafish via the Nrf2 Signaling Pathway"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3880-1586","authenticated-orcid":false,"given":"Wang","family":"Lin","sequence":"first","affiliation":[{"name":"College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China"},{"name":"Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming 650500, China"},{"name":"Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Changde 415000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wansheng","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China"},{"name":"Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Changde 415000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suqin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China"},{"name":"Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Changde 415000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengling","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China"},{"name":"Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Changde 415000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2243-4614","authenticated-orcid":false,"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jifeng","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinhong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China"},{"name":"Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Changde 415000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e2020WR029371","DOI":"10.1029\/2020WR029371","article-title":"Extreme climate anomalies enhancing cyanobacterial blooms in eutrophic Lake Taihu, China","volume":"57","author":"Qin","year":"2021","journal-title":"Water Resour. 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