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Their discovery could contribute to the development of new drugs for channelopathies and other diseases. Tf5 is a new peptide that has been identified from the venom of Tityus fasciolatus, a scorpion species endemic to the Brazilian Cerrado ecosystem. A full-length cDNA sequence of the Tf5 gene was obtained through a previously constructed transcriptomic library, where an ORF (Open Reading Frame) sequence with a length of 180 was found, including the 37 aa mature KTx domain, which has six Cys residues. Tf5 was purified from the crude venom, resulting in a peptide with a molecular mass of 3983.95 Da. Its K+ channel blocker activity was evaluated on Kv1.1, Kv1.2, Kv1.3, and Kv1.4 subtypes. Of these Kv channels, the peptide demonstrated an ability to block Kv1.2 and Kv1.3 with an IC50 of 15.53 nM and 116.41 nM, respectively. Additionally, Tf5 shares a high degree of sequence identity with toxins from the \u03b1-KTx4 subfamily, which led to it being classified as \u03b1-KTx4.9. This is the first Kv channel blocker described from the T. fasciolatus scorpion.<\/jats:p>","DOI":"10.3390\/toxins17020096","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T08:36:19Z","timestamp":1739867779000},"page":"96","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The First K+-Channel Blocker Described from Tityus fasciolatus Venom: The Purification, Molecular Cloning, and Functional Characterization of \u03b1-KTx4.9 (Tf5)"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6000-4700","authenticated-orcid":false,"given":"Isolda de Sousa","family":"Monteiro","sequence":"first","affiliation":[{"name":"Laboratory of Neuropharmacology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0136-0062","authenticated-orcid":false,"given":"Israel Flor Silva de","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thalita Soares","family":"Camargos","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil"},{"name":"Col\u00e9gio Militar de Bras\u00edlia, Bras\u00edlia 70790-020, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8713-0224","authenticated-orcid":false,"given":"Ernesto","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca 62210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo Carlos Barros de","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4306-3493","authenticated-orcid":false,"given":"Jonathan Dias","family":"Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6357-2661","authenticated-orcid":false,"given":"Lourival D.","family":"Possani","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca 62210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8239-4055","authenticated-orcid":false,"given":"Elisabeth Ferroni","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0962-099X","authenticated-orcid":false,"given":"Diogo Vieira","family":"Tibery","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10493-008-9203-5","article-title":"First Divergence Time Estimate of Spiders, Scorpions, Mites and Ticks (Subphylum: Chelicerata) Inferred from Mitochondrial Phylogeny","volume":"47","author":"Jeyaprakash","year":"2009","journal-title":"Exp. 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