{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:59:22Z","timestamp":1791831562601,"version":"4.3.4"},"reference-count":49,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:00:00Z","timestamp":1755129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nippon Beet Sugar Manufacturing Co., Ltd., Obihiro, Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Mycotoxin contamination in animal feed can cause acute or chronic adverse effects on growth, productivity, and immune function in livestock. This study aimed to evaluate the impact of difructose anhydride III (DFA III) supplementation on serum biochemical parameters and intestinal environment in Japanese Black (JB) breeding cows under low-level chronic dietary exposure to zearalenone (ZEN). Using urinary ZEN concentration as an indicator of exposure, 25 JB cows were selected from a breeding farm with confirmed natural feed contamination. Blood samples were collected before DFA III supplementation (day 0), and on days 20 and 40 post-supplementation. Serum biochemical parameters and short-chain fatty acid concentrations were measured. During the studies, dietary ZEN concentration increased, yet improvements were observed in liver function, nutritional status, immune response, and inflammatory markers. Notably, serum butyrate concentration significantly increased following DFA III administration. These findings suggest that DFA III may positively influence intestinal microflora and enhance intestinal barrier function, which could contribute to improved health and nutritional status in cattle exposed to low-level chronic dietary ZEN contamination. DFA III supplementation may represent a promising strategy for mitigating the effects of low-level mycotoxin exposure in livestock production systems.<\/jats:p>","DOI":"10.3390\/toxins17080409","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T15:16:37Z","timestamp":1755184597000},"page":"409","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6213-8844","authenticated-orcid":false,"suffix":"Jr.","given":"Topas Wicaksono","family":"Priyo","sequence":"first","affiliation":[{"name":"Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan"},{"name":"Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Sasazaki","sequence":"additional","affiliation":[{"name":"Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuki","family":"Toda","sequence":"additional","affiliation":[{"name":"Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Hasunuma","sequence":"additional","affiliation":[{"name":"Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisaku","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiko","family":"Kokushi","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0897-7804","authenticated-orcid":false,"given":"Seiichi","family":"Uno","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4430-5645","authenticated-orcid":false,"given":"Osamu","family":"Yamato","sequence":"additional","affiliation":[{"name":"Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0062, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Obi","sequence":"additional","affiliation":[{"name":"Joint Faculty of Veterinary Medicine, Kagoshima University, 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3422-1904","authenticated-orcid":false,"given":"Masayasu","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan"},{"name":"Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiro","family":"Takagi","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan"},{"name":"Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia"},{"name":"Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, 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