{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T15:16:58Z","timestamp":1772637418326,"version":"3.50.1"},"reference-count":56,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"R\u00e9gion Bretagne and SATT Ouest Valorisation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Mycotoxins are the most frequently occurring natural contaminant in food and feed products. Through the deployment of diverse agricultural strategies or biological, chemical, or physical treatments of crop products, mycotoxin contamination remains a persistent issue for the agricultural sector and food\/feed industry. We previously suggested that halophytes, thanks to their high antioxidant activity, could protect animal cell lines from mycotoxin contamination. Here, a hydroalcoholic extract of Calystegia soldanella L. leaves was evaluated for in vitro total antioxidant capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-quenching bioassays, as well as anti-inflammatory (ELISA measurement of IL-8 secretion), ROS-inhibiting production (CellROX Green assay), and calcium influx restoration (fluorescent probe Fura2-QBT assay) activities in two animal cells upon mycotoxin intoxication. C. soldanella extract displayed high antioxidant activities (DPPH IC50 &lt; 80 \u03bcg\u00b7mL\u22121 and TAC of 90 mg AAE\u00b7g\u22121 DW. Moreover, it exhibited a significant protective action on renal (MDBK) and intestinal (IPEC-J2) cells against zearalenone (ZEA) or T2-toxin contamination, restoring about 75% of cell viability (MTS bioassay) at 1 \u03bcg\u00b7mL\u22121. This effect was accompanied by strong anti-inflammatory, ROS-inhibition, and membrane integrity restoration activities. A bio-guided study revealed that the fraction of C. soldanella extract eluted from C18-bound silica with 60% methanol was the most active one. Upon HPLC and 1D- and 2D-NMR analyses, major compounds identified in this fraction were flavonol-type flavonoids, including quercetin-3-O-glucose (X1), quercetin-3-O-rutinoside (X2), and quercetin-3-O-glucose-6\u2033-acetate (X3). Enriched sub-fractions containing these compounds largely contributed to the cytoprotective effects of C. soldanella, supporting its potential use as a food\/feed ingredient.<\/jats:p>","DOI":"10.3390\/toxins18030127","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T16:06:59Z","timestamp":1772467619000},"page":"127","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sea Bindweed Prevents Mycotoxin Intoxication Through Antioxidant, Anti-Inflammatory and Cytoprotective Activities"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9346-9569","authenticated-orcid":false,"given":"Nolwenn","family":"Hymery","sequence":"first","affiliation":[{"name":"EA 3882 Laboratoire Universitaire de Biologie et Ecologie Microbienne, Universit\u00e9 de Bretagne Occidentale, CS 93837, F-29238 Brest, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Halima","family":"Boussaden","sequence":"additional","affiliation":[{"name":"EA 3882 Laboratoire Universitaire de Biologie et Ecologie Microbienne, Universit\u00e9 de Bretagne Occidentale, CS 93837, F-29238 Brest, France"},{"name":"Laboratoire G\u00e9oarchitecture_Territoires, Urbanisation, Biodiversit\u00e9, Environnement, Universit\u00e9 de Bretagne Occidentale, CS 93837, F-29238 Brest, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phane","family":"C\u00e9rantola","sequence":"additional","affiliation":[{"name":"Service G\u00e9n\u00e9ral des Plate-Formes Technologiques, Plateforme R\u00e9sonance Magn\u00e9tique Nucl\u00e9aire\u2013R\u00e9sonnance Paramagn\u00e9tique Electronique, Universit\u00e9 de Bretagne Occidentale, CS 93837, F-29238 Brest, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5974-1503","authenticated-orcid":false,"given":"Xavier","family":"Dauvergne","sequence":"additional","affiliation":[{"name":"Laboratoire G\u00e9oarchitecture_Territoires, Urbanisation, Biodiversit\u00e9, Environnement, Universit\u00e9 de Bretagne Occidentale, CS 93837, F-29238 Brest, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8295-6748","authenticated-orcid":false,"given":"Christian","family":"Magn\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire G\u00e9oarchitecture_Territoires, Urbanisation, Biodiversit\u00e9, Environnement, Universit\u00e9 de Bretagne Occidentale, CS 93837, F-29238 Brest, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"ref_1","unstructured":"FEFAC (2025, March 12). 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