{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:54:09Z","timestamp":1785405249589,"version":"3.56.0"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["WOT-02-001-064"],"award-info":[{"award-number":["WOT-02-001-064"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"award":["WOT-02-001-064"],"award-info":[{"award-number":["WOT-02-001-064"]}],"id":[{"id":"https:\/\/ror.org\/00k4n6c32","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Ochratoxin A (OTA) is a toxic metabolite produced by fungi, that can be present on various food products, cereals and plant-derived (raw) feed (materials). It was demonstrated that OTA has toxic effects after consumption by both animals and humans. Therefore, the European Food Safety Authority (EFSA) concluded that contribution to human exposure of OTA from (processed) meats should not be ignored. The objective of this study was to assess the occurrence of OTA; thus, data on OTA in cured meats were collected. An in-house validated analytical method using methanol extraction, clean-up with an immunoaffinity columns and LC-MS\/MS detection was applied. Quantification was done through the external calibration of standards in solvent using 13C20 OTA internal standard, with a reporting limit of 0.2 \u00b5g\/kg and LOQ of 0.04 \u00b5g\/kg. A total of 50 cured meat products were obtained from Dutch supermarkets. OTA was detected at or above the reporting limit in four samples of cured ham (range 0.30 \u00b5g\/kg to 79.8 \u00b5g\/kg) and two samples of sausages (0.2 \u00b5g\/kg and 0.41 \u00b5g\/kg). Overall, OTA was detected in twenty samples, and it was concluded that OTA occurred above the LOQ in 40% of cured meats analyzed.<\/jats:p>","DOI":"10.3390\/toxins18060262","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:58:12Z","timestamp":1781053092000},"page":"262","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Survey on Ochratoxin A Occurrence in Cured Meat Products in The Netherlands"],"prefix":"10.3390","volume":"18","author":[{"given":"Marta Magdalena","family":"Sopel","sequence":"first","affiliation":[{"name":"Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hester","family":"van den Top","sequence":"additional","affiliation":[{"name":"Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josipa","family":"Grzetic Martens","sequence":"additional","affiliation":[{"name":"Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"de Nijs","sequence":"additional","affiliation":[{"name":"Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.3390\/toxins2051078","article-title":"Biodegradation of ochratoxin A for food and feed decontamination","volume":"2","author":"Abrunhosa","year":"2010","journal-title":"Toxins"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4253","DOI":"10.3390\/toxins7104253","article-title":"Comparative Ochratoxin Toxicity: A Review of the Available Data","volume":"7","author":"Heussner","year":"2015","journal-title":"Toxins"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/0-387-28391-9_1","article-title":"Important mycotoxins and the fungi which produce them","volume":"571","author":"Frisvad","year":"2006","journal-title":"Adv. Food Mycol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/j.fm.2011.02.007","article-title":"Salting of dry-cured meat\u2013a potential cause of contamination with the ochratoxin A-producing species Penicillium nordicum","volume":"28","author":"Sonjak","year":"2011","journal-title":"Food Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.meatsci.2012.06.029","article-title":"Presence of ochratoxin A on the surface of dry-cured Iberian ham after initial fungal growth in the drying stage","volume":"92","author":"Rodriguez","year":"2012","journal-title":"Meat Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1080\/02652030210123869","article-title":"Ochratoxin A determination in ham by Immunoaffinity clean-up and a quick Fluorometric method","volume":"19","author":"Chiavaro","year":"2002","journal-title":"Food Add. Contam."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/BF02951509","article-title":"A new validated HPLC-FLD method for detecting ochratoxin A in dry-cured meat and in blue cheese","volume":"23","author":"Galaverna","year":"2007","journal-title":"Myco Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.jchromb.2007.05.010","article-title":"Determination of ochratoxin A in dry-cured meat products by a HPLC\u2013FLD quantitative method","volume":"855","author":"Toscani","year":"2007","journal-title":"J. Chromatogr. B"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"533","DOI":"10.3920\/WMJ2020.2568","article-title":"Ochratoxin A contamination of the casing and the edible portion of artisan salamis produced in two Italian regions","volume":"13","author":"Roncada","year":"2020","journal-title":"World Mycotoxin J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s12550-018-0338-x","article-title":"Occurrence of ochratoxin A in typical salami produced in different regions of Italy","volume":"35","author":"Altafini","year":"2019","journal-title":"Mycotoxin Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1080\/19440049.2016.1213428","article-title":"Assessment of possible human exposure to ochratoxin A in Croatia due to the consumption of dry-cured and fermented meat products","volume":"33","author":"Hengl","year":"2016","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"109016","DOI":"10.1016\/j.ijfoodmicro.2020.109016","article-title":"Influence of an industrial dry-fermented sausage processing on ochratoxin A production by Penicillium nordicum","volume":"339","author":"Delgado","year":"2021","journal-title":"Int. J. Food Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1007\/s12161-018-1231-0","article-title":"Gene expression analysis as a method to predict OTA accumulation in dry-cured meat products","volume":"11","author":"Delgado","year":"2018","journal-title":"Food Anal. Methods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1007\/s00216-010-4059-z","article-title":"Simultaneous determination of ochratoxin A, mycophenolic acid and fumonisin B2 in meat products","volume":"398","author":"Mogensen","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1080\/19393210.2015.1098735","article-title":"Ochratoxin A in artisan salami produced in Veneto (Italy)","volume":"9","author":"Armorini","year":"2016","journal-title":"Food Addit. Contam. Part B"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.fm.2008.07.006","article-title":"Moulds and ochratoxin A on surfaces of artisanal and industrial dry sausages","volume":"26","author":"Iacumin","year":"2009","journal-title":"Food Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1016\/j.foodchem.2009.11.036","article-title":"Occurrence of ochratoxin A in raw ham muscle, salami and dry-cured ham from pigs fed with contaminated diet","volume":"120","author":"Galaverna","year":"2010","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1111\/j.1745-4565.2011.00332.x","article-title":"Ochratoxigenic mold and ochratoxin A in fermented sausages from different areas in northern Italy: Occurrence, reduction or prevention with ozonated air","volume":"31","author":"Iacumin","year":"2011","journal-title":"J. Food Saf."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1080\/19440049.2016.1266395","article-title":"Moulds and mycotoxins detected in the regional speciality fermented sausage \u2018slavonski kulen\u2019 during a 1-year production period","volume":"34","author":"Pleadin","year":"2017","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_20","first-page":"95","article-title":"Natural occurrence of ochratoxin A in confined reared and grazing pigs derived products","volume":"25","author":"Meucci","year":"2019","journal-title":"Large Anim. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s12550-017-0305-y","article-title":"Monitoring of ochratoxin A and ochratoxin-producing fungi in traditional salami manufactured in Northern Italy","volume":"34","author":"Merla","year":"2018","journal-title":"Myco Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA) (2004). Opinion of the Scientific Panel on contaminants in the food chain [CONTAM] related to ochratoxin A (OTA) as undesirable substance in animal feed. Request No EFSA-Q-2003-039. EFSA J., 2, 101.","DOI":"10.2903\/j.efsa.2004.101"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA) (2006). Opinion of the Scientific Panel on contaminants in the food chain [CONTAM] related to ochratoxin A in food (Question N\u00b0 EFSA-Q-2005-154). EFSA J., 4, 365.","DOI":"10.2903\/j.efsa.2006.365"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"EFSA (2010). Statement on recent scientific information on the toxicity of ochratoxin A. EFSA J., 8, 1626.","DOI":"10.2903\/j.efsa.2010.1626"},{"key":"ref_25","unstructured":"EFSA (2020). Risk assessment of ochratoxin A in food. EFSA J., 18, e06113."},{"key":"ref_26","unstructured":"EU (2026, January 04). Commission Regulation (EU) 2023\/915 of 25 April 2023 on Maximum Levels for Certain Contaminants in Food and Repealing Regulation (EC) No 1881\/2006. 2023. OJ, 2023, L 119, 3. Available online: http:\/\/data.europa.eu\/eli\/reg\/2023\/915\/2024-04-25."},{"key":"ref_27","unstructured":"EU (2006). Commission Recommendation of 17 August 2006 on the Presence of Deoxynivalenol, Zearalenone, Ochratoxin A, T-2 and HT-2 and Fumonisins in Products Intended for Animal Feeding (2006\/576\/EC). OJ, L 229, 7. Available online: http:\/\/data.europa.eu\/eli\/reco\/2006\/576\/2016-08-02."},{"key":"ref_28","unstructured":"EU (2017). Regulation (EU) 2017\/625 of the European Parliament and of the Council of 15 March 2017 on Official Controls and Other Official Activities Performed to Ensure the Application of Food and Feed Law, Rules on Animal Health and Welfare, Plant Health and Plant Protection Products, Amending Regulations (EC) No 999\/2001, (EC) No 396\/2005, (EC) No 1069\/2009, (EC) No 1107\/2009, (EU) No 1151\/2012, (EU) No 652\/2014, (EU) 2016\/429 and (EU) 2016\/2031 of the European Parliament and of the Council, Council Regulations (EC) No 1\/2005 and (EC) No 1099\/2009 and Council Directives Council 98\/58\/EC, 1999\/74\/EC, 2007\/43\/EC, 2008\/119\/EC and 2008\/120\/EC, and repealing Directives 89\/608\/EEC, 89\/662\/EEC, 90\/425\/EEC, 91\/496\/EEC, 96\/23\/EC, Regulations (EC) No 854\/2004 and (EC) No 882\/2004 of the European Parliament and of the Council, 96\/93\/EC and 97\/78\/EC and Council Decision 92\/438\/EEC (Official Controls Regulation). OJ, L95, 163. Available online: http:\/\/data.europa.eu\/eli\/reg\/2017\/625\/oj."},{"key":"ref_29","first-page":"52","article-title":"Direttive in materia di controllo ufficiale sui prodotti alimentari: Valori massimi ammissibili di micotossine nelle derrate alimentari di origine nazionale, comunitaria e Paesi Terzi","volume":"135","year":"1999","journal-title":"Gazz. Uff."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.tvjl.2011.11.002","article-title":"Food safety implications of ochratoxin A in animal-derived food products","volume":"192","author":"Duarte","year":"2012","journal-title":"Vet. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1080\/02652030110113807","article-title":"Content of ochratoxin A in paired kidney and meat samples from healthy Danish slaughter pigs","volume":"19","author":"Petersen","year":"2002","journal-title":"Food Add. Contam."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1515\/jvetres-2017-0071","article-title":"Occurrence of ochratoxin A in animal tissues and feeds in Poland in 2014\u20132016","volume":"61","author":"Pietruszka","year":"2017","journal-title":"J. Vet. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.chroma.2005.07.020","article-title":"Determination of ochratoxin A at part-per-trillion level in Italian salami by immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection","volume":"1090","author":"Monaci","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.foodcont.2014.12.027","article-title":"Survey of aflatoxin B1 and ochratoxin A occurrence in traditional meat products coming from Croatian households and markets","volume":"52","author":"Pleadin","year":"2015","journal-title":"Food Control"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ijfoodmicro.2016.03.028","article-title":"Study of gene expression and OTA production by Penicillium nordicum during a small-scale seasoning process of salami","volume":"227","author":"Ferrara","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Dada, T.A., Ekwomadu, T.I., and Mwanza, M. (2020). Multi mycotoxin determination in dried beef using Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry (LC-MS\/MS). Toxins, 12.","DOI":"10.3390\/toxins12060357"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2336","DOI":"10.1111\/jam.15114","article-title":"Mycobiota dynamics and mycotoxin detection in PGI Salame Piemonte","volume":"13","author":"Franciosa","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"108459","DOI":"10.1016\/j.ijfoodmicro.2019.108459","article-title":"A study of surface moulds and mycotoxins in Croatian traditional dry-cured meat products","volume":"317","author":"Zadravec","year":"2020","journal-title":"Int. J. Food Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"109850","DOI":"10.1016\/j.meatsci.2025.109850","article-title":"Mycotoxins in meat products","volume":"227","author":"Andrade","year":"2025","journal-title":"Meat Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Chen, Y., Chen, J., Zhu, Q., and Wan, J. (2022). Ochratoxin A in dry-cured ham: OTA-producing fungi, prevalence, detection methods, and biocontrol strategies\u2014A review. Toxins, 14.","DOI":"10.3390\/toxins14100693"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.foodcont.2013.04.011","article-title":"Direct and indirect contamination with ochratoxin A of ripened pork products","volume":"34","author":"Bertuzzi","year":"2013","journal-title":"Food Control"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1080\/02652030500198027","article-title":"Aflatoxin and ochratoxin A content of spices in Hungary","volume":"22","author":"Fazekas","year":"2005","journal-title":"Food Add. Contam."},{"key":"ref_43","unstructured":"WFSR (2023, August 16). EURLMP-Method_016 v1, Determination of Ochratoxin A in Meat and Cheese by LC-MS\/MS."},{"key":"ref_44","unstructured":"SANTE, D.G._ (2022, May 02). Guidance SANTE 11312\/2021\u2014Analytical Quality Control and Method Validation Procedures for Pesticide Residues Analysis in Food and Feed. Available online: https:\/\/www.eurl-pesticides.eu\/userfiles\/file\/EurlALL\/SANTE-11312_2021-V2.pdf."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/18\/6\/262\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T12:28:04Z","timestamp":1781180884000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/18\/6\/262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,9]]},"references-count":44,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["toxins18060262"],"URL":"https:\/\/doi.org\/10.3390\/toxins18060262","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,9]]}}}