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Enzymatic detoxification enables structure-targeted transformation of toxicity-determining motifs, such as epoxide rings, reactive double bonds, amide linkages, and lactone structures. However, free mycotoxin-degrading enzymes are often constrained by poor operational stability, difficult recovery, and limited adaptability to complex matrices. Metal\u2013organic frameworks (MOFs) provide programmable microenvironments for enzyme immobilization through tunable pore structures, interfacial chemistry, and confinement effects. This review links toxic structural motifs with enzymatic transformation targets, discusses MOF\u2013enzyme interface engineering and representative host\u2013enzyme compatibility, and evaluates application modes including single-enzyme systems, multi-enzyme co-immobilization or cascade systems, adsorption\u2013degradation coupling, and detection\u2013degradation integration. Key bottlenecks involving enzyme leakage, mass-transfer limitation, real-matrix stability, scalable preparation, and biosafety are critically discussed. Rather than treating MOFs as passive enzyme carriers, this review proposes an application-oriented framework that integrates toxin structure, enzyme function, MOF interface regulation, matrix compatibility, and safety validation to guide the development of MOF-immobilized degrading enzymes for practical mycotoxin detoxification.<\/jats:p>","DOI":"10.3390\/toxins18080353","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T11:04:12Z","timestamp":1787137452000},"page":"353","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metal\u2013Organic Framework-Immobilized Mycotoxin-Degrading Enzymes: Interfaces, Host Design, and Food\/Feed Applications"],"prefix":"10.3390","volume":"18","author":[{"given":"Boyu","family":"Fang","sequence":"first","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6683-1956","authenticated-orcid":false,"given":"Miao","family":"Long","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100544","DOI":"10.1016\/j.japr.2025.100544","article-title":"Review article: Recent advances in enzyme technologies for mitigating mycotoxin contamination in poultry feed","volume":"34","author":"Aleschko","year":"2025","journal-title":"J. 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