{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:23:22Z","timestamp":1788341002801,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Wheat, soy and pea protein isolates used for the production of plant-based meat alternatives were analyzed for 87 mycotoxins and 26 plant toxins by means of a validated QuEChERS-based LC-MS\/MS multi-method. For wheat gluten, the results revealed the presence of Alternaria toxins, deoxynivalenol, enniatins, ergot alkaloids and ochratoxin A (OTA). For soy protein, beauvericin, enniatin B and OTA were the most frequently detected mycotoxins, while for pea protein, OTA was the most frequently detected mycotoxin. From plant toxins, the tropane alkaloids atropine and scopolamine were detected in wheat gluten and soy protein. The emerging Alternaria toxin alterperylenol was reported for the first time in wheat gluten (85% of samples). OTA was present at high levels in all three matrices (exceeding the EU maximum level in one wheat gluten sample). Ergot alkaloids were found in 95% of wheat gluten samples, but none exceeded the ML. The highest co-occurrence of different toxins was observed in wheat gluten (median: 22, max: 27 toxins\/sample), followed by soy protein (median: five, max: nine toxins\/sample) and pea protein (median: one, max: five toxins\/sample).<\/jats:p>","DOI":"10.3390\/toxins18090377","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:56:01Z","timestamp":1788339361000},"page":"377","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of 113 Mycotoxins and Plant Toxins in Wheat, Soy and Pea Protein Concentrates from the German Market Reveals, i.a., a High Prevalence of Alterperylenol in Wheat Gluten"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2012-5893","authenticated-orcid":false,"given":"Ahmed H.","family":"El-Khatib","sequence":"first","affiliation":[{"name":"German Federal Institute for Risk Assessment (BfR), Reference Centre for Food and Feed Analysis, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6860-4535","authenticated-orcid":false,"given":"Arnold","family":"Bahlmann","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (BfR), Reference Centre for Food and Feed Analysis, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Christoph","family":"Hutzler","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (BfR), Reference Centre for Food and Feed Analysis, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4197-5758","authenticated-orcid":false,"given":"Stefan","family":"Weigel","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (BfR), Reference Centre for Food and Feed Analysis, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100551","DOI":"10.1016\/j.fufo.2025.100551","article-title":"Future Trends in Plant-Based Meat: Consumer Perception, Market Growth and Health Benefits","volume":"11","author":"Xavier","year":"2025","journal-title":"Future Foods"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e14758","DOI":"10.1111\/jfpe.14758","article-title":"Plant Protein Resources, Novel Extraction and Precipitation Methods: A Review","volume":"47","author":"Tekin","year":"2024","journal-title":"J. Food Process Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/nbu.12450","article-title":"Plant Protein Ingredients with Food Functionality Potential","volume":"45","author":"Loveday","year":"2020","journal-title":"Nutr. Bull."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1039\/D3FB00003F","article-title":"Sustainable Plant Protein: An up-to-Date Overview of Sources, Extraction Techniques and Utilization","volume":"1","author":"Chandran","year":"2023","journal-title":"Sustain. Food Technol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-\u00c1lvarez, A.J., Mondor, M., and Nosworthy, M.G. (2023). Green Protein Processing Technologies from Plants. Green Protein Processing Technologies from Plants, Springer.","DOI":"10.1007\/978-3-031-16968-7"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111490","DOI":"10.1016\/j.foodres.2022.111490","article-title":"A Systematic Review of Natural Toxins Occurrence in Plant Commodities Used for Plant-Based Meat Alternatives Production","volume":"158","author":"Dellafiora","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115422","DOI":"10.1016\/j.foodres.2024.115422","article-title":"Risk Ranking of Mycotoxins in Plant-Based Meat and Dairy Alternatives under Protein Transition Scenarios","volume":"200","author":"Schryvers","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Janik, E., Niemcewicz, M., Ceremuga, M., Stela, M., Saluk-Bijak, J., Siadkowski, A., and Bijak, M. (2020). Molecular Aspects of Mycotoxins-a Serious Problem for Human Health. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21218187"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Polak-Sliwinska, M., and Paszczyk, B. (2021). Trichothecenes in Food and Feed, Relevance to Human and Animal Health and Methods of Detection: A Systematic Review. Molecules, 26.","DOI":"10.3390\/molecules26020454"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/carcin\/bgp264","article-title":"Mycotoxins and Human Disease: A Largely Ignored Global Health Issue","volume":"31","author":"Wild","year":"2010","journal-title":"Carcinogenesis"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.toxlet.2022.10.007","article-title":"The Combined Effect of Deoxynivalenol and Fumonisin B(1) on Small Intestinal Inflammation Mediated by Pyroptosis in Vivo and in Vitro","volume":"372","author":"Mao","year":"2023","journal-title":"Toxicol. Lett."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"EFSA (2011). Scientific Opinion on Pyrrolizidine Alkaloids in Food and Feed. EFSA J., 9, 2406.","DOI":"10.2903\/j.efsa.2011.2406"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1055\/a-2218-5667","article-title":"Toxins in Botanical Drugs and Plant-Derived Food and Feed\u2014From Science to Regulation: A Workshop Review","volume":"90","author":"Schrenk","year":"2024","journal-title":"Planta Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Alvito, P., and Pereira-da-Silva, L. (2022). Mycotoxin Exposure during the First 1000 Days of Life and Its Impact on Children\u2019s Health: A Clinical Overview. Toxins, 14.","DOI":"10.3390\/toxins14030189"},{"key":"ref_15","unstructured":"European Commission (2023). Commission Regulation (Eu) 2023\/915 of 25 April 2023 on Maximum Levels for Certain Contaminants in Food and Repealing Regulation (Ec) No 1881\/2006, Official Journal of the European Union."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mihalache, O.A., De Boevre, M., Dellafiora, L., De Saeger, S., Moretti, A., Pinson-Gadais, L., Ponts, N., Richard-Forget, F., Susca, A., and Dall\u2019Asta, C. (2023). The Occurrence of Non-Regulated Mycotoxins in Foods: A Systematic Review. Toxins, 15.","DOI":"10.3390\/toxins15090583"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.lwt.2018.09.046","article-title":"Development of an Uhplc-Q-Orbitrap Hrms Method for Simultaneous Determination of Mycotoxins and Isoflavones in Soy-Based Burgers","volume":"99","author":"Castaldo","year":"2019","journal-title":"LWT"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"112766","DOI":"10.1016\/j.foodres.2023.112766","article-title":"Multi-Mycotoxin Determination in Plant-Based Meat Alternatives and Exposure Assessment","volume":"168","author":"Cutroneo","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"108537","DOI":"10.1016\/j.envint.2024.108537","article-title":"Occurrence of Mycotoxins in Meat Alternatives: Dietary Exposure, Potential Health Risks, and Burden of Disease","volume":"185","author":"Torrijos","year":"2024","journal-title":"Env. Int."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"EFSA (2013). Scientific Opinion on Tropane Alkaloids in Food and Feed. EFSA J., 11, 3386.","DOI":"10.2903\/j.efsa.2013.3386"},{"key":"ref_21","unstructured":"European Commission (2023). Commission Implementing Regulation (Eu) 2023\/2782 of 14 December 2023 Laying Down the Methods of Sampling and Analysis for the Control of the Levels of Mycotoxins in Food and Repealing Regulation (Ec) No 401\/2006, Official Journal of the European Union."},{"key":"ref_22","unstructured":"European Commission (2023). Commission Implementing Regulation (Eu) 2023\/2783 of 14 December 2023 Laying Down the Methods of Sampling and Analysis for the Control of the Levels of Plant Toxins in Food and Repealing Regulation (Eu) 2015\/705, Official Journal of the European Union."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5047","DOI":"10.1007\/s00216-025-06024-6","article-title":"A Validated Lc-Ms\/Ms Multi-Method for the Determination of 110 Mycotoxins and Plant Toxins in Cow Milk and Application to Samples from Germany","volume":"417","author":"Bahlmann","year":"2025","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"12384","DOI":"10.1021\/acs.jafc.5c16175","article-title":"Multi-Mycotoxin Quantification in Soy Protein Powders, Soymilk, Soybeans (Glycine Max) and Other Legumes Using a New Targeted Differential Ion Mobility Lcms Method","volume":"74","author":"Shu","year":"2026","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Sandlin, N., Lee, J., Zaccaria, M., Domin, M., and Momeni, B. (2025). Degradation of Aflatoxins in Weakly Alkaline Conditions. Front. Sustain. Food Syst., 8.","DOI":"10.3389\/fsufs.2024.1445232"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.foodchem.2017.12.057","article-title":"Hydrolysers of Modified Mycotoxins in Maize: Alpha-Amylase and Cellulase Induce an Underestimation of the Total Aflatoxin Content","volume":"248","author":"Vidal","year":"2018","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s12550-016-0257-7","article-title":"Impact of Food Processing and Detoxification Treatments on Mycotoxin Contamination","volume":"32","author":"Karlovsky","year":"2016","journal-title":"Mycotoxin Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"EFSA (2012). Scientific Opinion on Ergot Alkaloids in Food and Feed. EFSA J., 10, 2798.","DOI":"10.2903\/j.efsa.2012.2798"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.3390\/toxins7062024","article-title":"Analysis of Ergot Alkaloids","volume":"7","author":"Crews","year":"2015","journal-title":"Toxins"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1023\/A:1014050926916","article-title":"The Chemistry of Peptide Ergot Alkaloids. Part 1. Classification and Chemistry of Ergot Peptides","volume":"35","author":"Komarova","year":"2001","journal-title":"Pharm. Chem. J."},{"key":"ref_31","unstructured":"EFSA (2020). Risk Assessment of Ochratoxin a in Food. EFSA J., 18, e06113."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1111\/1541-4337.12338","article-title":"The Fate of Mycotoxins during the Processing of Wheat for Human Consumption","volume":"17","author":"Schaarschmidt","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"19844","DOI":"10.1039\/C9RA02086A","article-title":"Comprehensive Study on the Degradation of Ochratoxin a in Water by Spectroscopic Techniques and Dft Calculations","volume":"9","author":"Cagnasso","year":"2019","journal-title":"RSC Adv."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.foodcont.2019.03.019","article-title":"Quantitation of Free and Modified Mycotoxins in European Food Products by Lc-Ms\/Ms","volume":"102","author":"Puntscher","year":"2019","journal-title":"Food Control"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s00204-024-03877-1","article-title":"Discovery of the Mycotoxins Alterperylenol and Altertoxin I as Novel Immunosuppressive and Antiestrogenic Compounds in Vitro","volume":"99","author":"Crudo","year":"2025","journal-title":"Arch. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Zwickel, T., Kahl, S.M., Rychlik, M., and M\u00fcller, M.E.H. (2018). Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi\u2014Do Multitoxin Mixtures Act as an Indicator for Species Differentiation?. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01368"},{"key":"ref_37","first-page":"1177E","article-title":"Generation of Occurrence Data on Citrinin in Food","volume":"14","author":"Spanjerb","year":"2017","journal-title":"EFSA Support. Publ."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1080\/19393210.2017.1389994","article-title":"Co-Occurrence of Ochratoxin a and Citrinin in Unprocessed Cereals Established during a Three-Year Investigation Period","volume":"11","author":"Pleadin","year":"2018","journal-title":"Food Addit. Contam. Part B Surveill."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"108245","DOI":"10.1016\/j.foodcont.2021.108245","article-title":"Evaluation of the Underestimation of Citrinin Content in Hongqu Using Hydrolysis Treatments and Uplc-Fld","volume":"130","author":"Hou","year":"2021","journal-title":"Food Control"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1080\/19440049.2016.1228126","article-title":"Occurrence of Beauvericin and Enniatins in Wheat Flour and Corn Grits on the Japanese Market, and Their Co-Contamination with Type B Trichothecene Mycotoxins","volume":"33","author":"Yoshinari","year":"2016","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"EFSA (2014). Scientific Opinion on the Risks to Human and Animal Health Related to the Presence of Beauvericin and Enniatins in Food and Feed. EFSA J., 12, 3802.","DOI":"10.2903\/j.efsa.2014.3802"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/s41538-024-00303-9","article-title":"Unraveling the Fate of Mycotoxins during the Production of Legume Protein and Other Derived Products","volume":"8","author":"Pavicich","year":"2024","journal-title":"npj Sci. Food"},{"key":"ref_43","unstructured":"EFSA (2017). Risks to Human and Animal Health Related to the Presence of Deoxynivalenol and Its Acetylated and Modified Forms in Food and Feed. EFSA J., 15, e04718."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"(2011). EFSA Panel on Contaminants in the Food Chain. Scientific Opinion on the Risks for Public Health Related to the Presence of Zearalenone in Food Efsa Panel on Contaminants in the Food Chain. EFSA J., 9, 2197.","DOI":"10.2903\/j.efsa.2011.2197"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Jonard, C., Chandelier, A., Eylenbosch, D., Pannecoucque, J., Godin, B., Douny, C., Scippo, M.L., and Gofflot, S. (2025). Multi-Mycotoxin Analyses by Uplc-Ms\/Ms in Wheat: The Situation in Belgium in 2023 and 2024. Foods, 14.","DOI":"10.3390\/foods14132300"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"110963","DOI":"10.1016\/j.foodcont.2024.110963","article-title":"Detection of Tropane Alkaloid Contaminations in Unprocessed Soybeans and Their Fate in Food and Feed Processing","volume":"168","author":"Ranetbauer","year":"2025","journal-title":"Food Control"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1021\/acs.analchem.9b04922","article-title":"Tale of Two Alkaloids: Ph-Controlled Electrochemiluminescence for Differentiation of Structurally Similar Compounds","volume":"92","author":"Brown","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Ji, X., Jin, C., Xiao, Y., Deng, M., Wang, W., Lyu, W., Chen, J., Li, R., Li, Y., and Yang, H. (2023). Natural Occurrence of Regulated and Emerging Mycotoxins in Wheat Grains and Assessment of the Risks from Dietary Mycotoxins Exposure in China. Toxins, 15.","DOI":"10.3390\/toxins15060389"},{"key":"ref_49","unstructured":"EURL-MP (2026). Eurl-MP-Guidance Doc_003 (Version 1.4). Guidance Document on Performance Criteria for Methods of Analysis for Mycotoxins and Plant Toxins in Food and Feed, Wageningen Food and Safety Research."},{"key":"ref_50","unstructured":"Wenzl, T., Haedrich, J., Schaechtele, A., Robouch, P., and Stroka, J. (2016). Guidance Document on the Estimation of LOD and LOQ for Measurements in the Field of Contaminants in Feed and Food, Publications Office of the European Union."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/18\/9\/377\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:03:35Z","timestamp":1788339815000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/18\/9\/377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":50,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["toxins18090377"],"URL":"https:\/\/doi.org\/10.3390\/toxins18090377","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]}}}