{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:08:25Z","timestamp":1788858505937,"version":"build-2803163510"},"reference-count":54,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Program for Scientific Research and Advanced Studies","award":["PE501084302-2023-PROCIENCIA-BM"],"award-info":[{"award-number":["PE501084302-2023-PROCIENCIA-BM"]}]},{"award":["PE501084302-2023-PROCIENCIA-BM"],"award-info":[{"award-number":["PE501084302-2023-PROCIENCIA-BM"]}],"id":[{"id":"https:\/\/ror.org\/05pap9b79","id-type":"ROR","asserted-by":"publisher"}]},{"name":"the Universidad Aut\u00f3noma de Aguascalientes","award":["Project PIP\/SA 25-1"],"award-info":[{"award-number":["Project PIP\/SA 25-1"]}]},{"award":["Project PIP\/SA 25-1"],"award-info":[{"award-number":["Project PIP\/SA 25-1"]}],"id":[{"id":"https:\/\/ror.org\/03ec8vy26","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Peruvian Government","award":["026-INNOVATEPERU-PITEI-2017"],"award-info":[{"award-number":["026-INNOVATEPERU-PITEI-2017"]}]},{"award":["026-INNOVATEPERU-PITEI-2017"],"award-info":[{"award-number":["026-INNOVATEPERU-PITEI-2017"]}],"id":[{"id":"https:\/\/ror.org\/05n3rrk65","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The strategy of adding mycotoxin binders (MTBs) to feed lactating cows to reduce the absorption of aflatoxins (AFs) in the gastrointestinal tract and reduce milk contamination by hydroxylated AF compounds, especially AFM1, to below the maximum contaminant level (MCL) has become widespread. However, the indiscriminate use of MTBs is very common. The objective of the study was to determine, under field conditions, the efficacy of an MTB in reducing AFM1 contamination in cow\u2019s milk. Feed and raw milk were evaluated on-farm using lateral flow immunochromatography devices. In a 2 \u00d7 2 Latin square design, in two separate test periods, two diets were administered consecutively to each of twelve Holstein cows (multiparous, 220 \u00b1 20 lactation days): 1: Basal diet naturally contaminated (120 AFB1 \u00b5g\/d); 2: Basal diet plus an MTB (calcium carbonate and yeast fragments; 150 g\/d). MTB intake effectively reduced the mean concentration, daily excretion, and percentage transfer rate of AFM1 in milk (0.724 \u00b1 0.045 to 0.479 \u00b1 0.055 \u00b5g\/kg, p &lt; 0.01; 15.6 \u00b1 1.7 to 9.7 \u00b1 1.5 \u00b5g\/d, p &lt; 0.05; and 13.0 \u00b1 1.4 to 8.1 \u00b1 1.3%, p &lt; 0.05, respectively). These results highlight the need to evaluate, under field conditions, the effectiveness of MTBs as part of a mycotoxin contamination control program on dairy farms.<\/jats:p>","DOI":"10.3390\/toxins18090386","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:30:46Z","timestamp":1788777046000},"page":"386","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Efficacy of an Aflatoxin Binder in Holstein Dairy Cows in Per\u00fa Under Field Conditions"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8444-8694","authenticated-orcid":false,"given":"Ivonne","family":"Salazar-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Av. 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La Molina S\/N, La Molina, Lima 12, Peru"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9236-8141","authenticated-orcid":false,"given":"Patricia","family":"Glorio-Paulet","sequence":"additional","affiliation":[{"name":"Facultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Av. La Molina S\/N, La Molina, Lima 12, Peru"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1108-6359","authenticated-orcid":false,"given":"Isabel Cristina","family":"Molina-Botero","sequence":"additional","affiliation":[{"name":"Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Av. La Molina S\/N, La Molina, Lima 12, Peru"},{"name":"Tropical Forages Program of the International Center for Tropical Agriculture (CIAT), Km 17 Palmira, Valle del Cauca 763022, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9354-0588","authenticated-orcid":false,"given":"Arturo Gerardo","family":"Valdivia-Flores","sequence":"additional","affiliation":[{"name":"Agricultural Sciences Centre, Universidad Aut\u00f3noma de Aguascalientes, Av. Universidad 940, Aguascalientes 20100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9021-5838","authenticated-orcid":false,"given":"Carlos","family":"G\u00f3mez-Bravo","sequence":"additional","affiliation":[{"name":"Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Av. La Molina S\/N, La Molina, Lima 12, Peru"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4067989","DOI":"10.1155\/2017\/4067989","article-title":"Seasonally feed-related aflatoxins B1 and M1 spread in semiarid industrial dairy herd and its deteriorating impacts on food and immunity","volume":"2017","author":"Mozafari","year":"2017","journal-title":"J. Food Qual."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.47278\/journal.abr\/2020.006","article-title":"Mycotoxins\u2013a global one health concern: A review","volume":"2","author":"Imran","year":"2020","journal-title":"Agrobiol. Rec."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.tvjl.2007.12.034","article-title":"The role of mycotoxins in the health and performance of dairy cows","volume":"176","year":"2008","journal-title":"Vet. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.29261\/pakvetj\/2020.075","article-title":"Diagnosis of subclinical aflatoxicosis by biochemical changes in dairy cows under field conditions","volume":"41","year":"2021","journal-title":"Pak. Vet. J."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"de Luna-L\u00f3pez, M.C., Valdivia-Flores, A.G., Rangel-Mu\u00f1oz, E.J., Hern\u00e1ndez-Valdivia, E., Quezada-Trist\u00e1n, T., Jaramillo-Ju\u00e1rez, F., and Ortiz-Mart\u00ednez, R. (2025). Time-dependent changes in performance, biochemistry, and histology in dairy calves with acute Aflatoxicosis. Vet. Sci., 12.","DOI":"10.3390\/vetsci12030273"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Guo, W., Fan, Z., Fan, K., Meng, J., Nie, D., Tangni, E.K., Li, Z., Zhao, Z., and Han, Z. (2021). In vivo kinetics and biotransformation of aflatoxin B1 in dairy cows based on the establishment of a reliable UHPLC-MS\/MS method. Front. Chem., 9.","DOI":"10.3389\/fchem.2021.809480"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Benkerroum, N. (2020). Chronic and acute toxicities of aflatoxins: Mechanisms of action. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17020423"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.3168\/jds.S0022-0302(05)72987-8","article-title":"Transfer of aflatoxin B1 from feed to milk and from milk to curd and whey in dairy sheep fed artificially contaminated concentrates","volume":"88","author":"Battacone","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.22319\/rmcp.v5i1.3062","article-title":"Comparison of methods to evaluate aflatoxin B1 exposure in dairy cattle and the effect of mycotoxin adsorbents to reduce AFM1 residues in milk","volume":"5","author":"Rojo","year":"2014","journal-title":"Rev. Mex. Cienc. Pecu."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.3168\/jds.2013-7842","article-title":"Transfer of dietary aflatoxin B1 to milk aflatoxin M1 and effect of inclusion of adsorbent in the diet of dairy cows","volume":"98","author":"Xiong","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1111\/1471-0307.12382","article-title":"Recent aflatoxin survey data in milk and milk products: A review","volume":"70","author":"Ketney","year":"2017","journal-title":"Int. J. Dairy Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1016\/j.fct.2007.10.005","article-title":"On the occurrence of aflatoxin M1 in milk and dairy products","volume":"47","author":"Prandini","year":"2009","journal-title":"Food Chem. Toxicol."},{"key":"ref_13","first-page":"13","article-title":"Outbreak of aflatoxicosis on a local cattle farm in Pakistan","volume":"3","author":"Umar","year":"2015","journal-title":"Veterinaria"},{"key":"ref_14","first-page":"492","article-title":"Mycotoxin contaminating maize and grass silages for dairy cattle feeding: Current state and challenges","volume":"3","author":"Tangni","year":"2013","journal-title":"J. Anim. Sci. Adv."},{"key":"ref_15","first-page":"435","article-title":"Characterization of Aspergillus flavus and quantification of aflatoxins in feed and raw milk of cows in Aguascalientes, Mexico","volume":"11","year":"2020","journal-title":"Rev. Mex. Cienc. Pecu."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.1080\/10408398.2019.1658570","article-title":"Worldwide contamination of food-crops with mycotoxins: Validity of the widely cited \u2018FAO estimate\u2019 of 25%","volume":"60","author":"Eskola","year":"2020","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_17","unstructured":"U.S. Food and Drug Administration (FDA) (2026, April 24). Guidance for Industry: Action Levels for Poisonous or Deleterious Substances in Human Food and Animal Feed, Available online: https:\/\/www.fda.gov\/regulatory-information\/search-fda-guidance-documents\/guidance-industry-action-levels-poisonous-or-deleterious-substances-human-food-and-animal-feed#afla."},{"key":"ref_18","unstructured":"Commission Regulation (2023). Commission Regulation (EU) 2023\/915 of 25 April 2023 on maximum levels for certain contaminants in food and repealing Regulation (EC) No 1881\/2006. Off. J. Eur. Union, 119, 103\u2013157."},{"key":"ref_19","unstructured":"U.S. Food and Drug Administration (FDA) (2026, April 14). Action Levels for Aflatoxins in Animal Food, Compliance Policy Guide, Available online: https:\/\/www.fda.gov\/media\/121202\/download."},{"key":"ref_20","unstructured":"Codex Alimentarius Commission, FAO, and WHO (2026, June 01). General Standard for Contaminants and Toxins in Food and Feed (CXS 193-1995). Available online: https:\/\/www.fao.org\/fao-who-codexalimentarius\/thematic-areas\/contaminants\/en\/."},{"key":"ref_21","unstructured":"European Commission (2009). Commission Regulation (EC) No 386\/2009 of 12 May 2009 amending Regulation (EC) No 1831\/2003 as regards the establishment of a new functional group of feed additives. Off. J. Eur. Union, L118, 66. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/PDF\/?uri=CELEX:02003R1831-20210327."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1023\/B:MYCO.0000020587.93872.59","article-title":"Aflatoxin binders II: Reduction of aflatoxin M1 in milk by sequestering agents of cows consuming aflatoxin in feed","volume":"157","author":"Hagler","year":"2004","journal-title":"Mycopathologia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6263","DOI":"10.3168\/jds.2016-10905","article-title":"Effects of three sequestering agents on milk aflatoxin M1 concentration and the performance and immune status of dairy cows fed diets artificially contaminated with aflatoxin B1","volume":"99","author":"Ogunade","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6614","DOI":"10.3168\/jds.2018-16151","article-title":"Feed additives containing sequestrant clay minerals and inactivated yeast reduce aflatoxin excretion in milk of dairy cows","volume":"102","author":"Rodrigues","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.3168\/jds.2019-17561","article-title":"In vitro assessment of the capacity of certain mycotoxin binders to adsorb some amino acids and water-soluble vitamins","volume":"103","author":"Kihal","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_26","unstructured":"Market Growth Reports (2026, April 14). Feed Mycotoxin Binders and Modifiers Market. Market Growth Reports. Available online: https:\/\/www.marketgrowthreports.com\/market-reports\/feed-mycotoxin-binders-and-modifiers-market-112296."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Shabeer, S., Asad, S., Jamal, A., and Ali, A. (2022). Aflatoxin contamination, its impact and management strategies: An updated review. Toxins, 14.","DOI":"10.3390\/toxins14050307"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Rangel-Mu\u00f1oz, E.J., Valdivia-Flores, A.G., Hern\u00e1ndez-Delgado, S., Cruz-V\u00e1zquez, C., de-Luna-L\u00f3pez, M.C., Quezada-Trist\u00e1n, T., Ortiz-Mart\u00ednez, R., and Mayek-P\u00e9rez, N. (2022). Assessment of the potential of a native non-aflatoxigenic Aspergillus flavus isolate to reduce aflatoxin contamination in dairy feed. Toxins, 14.","DOI":"10.3390\/toxins14070437"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s12550-016-0257-7","article-title":"Impact of food processing and detoxification treatments on mycotoxin contamination","volume":"32","author":"Karlovsky","year":"2016","journal-title":"Mycotoxin Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Gong, A., Song, M., and Zhang, J. (2024). Current strategies in controlling Aspergillus flavus and aflatoxins in grains during storage: A review. Sustainability, 16.","DOI":"10.3390\/su16083171"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e71104","DOI":"10.1002\/fsn3.71104","article-title":"Aflatoxin contamination in agri-food systems: A comprehensive review of toxicity, food security, economic impacts, and sustainable mitigation across the value chain","volume":"13","author":"Yohannis","year":"2025","journal-title":"Food Sci. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1080\/15569543.2017.1354306","article-title":"Simultaneous analysis of mycotoxins in corn flour using LC\/MS-MS combined with a modified QuEChERS procedure","volume":"37","author":"Amirahmadi","year":"2018","journal-title":"Toxin Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1093\/jaoacint\/qsac035","article-title":"Analysis of Aflatoxins, fumonisins, deoxynivalenol, ochratoxin A, zearalenone, HT-2, and T-2 toxins in animal feed by LC\u2013MS\/MS using cleanup with a multi-antibody immunoaffinity column","volume":"105","author":"Mackay","year":"2022","journal-title":"J. AOAC Int."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Serraino, A., Bonilauri, P., Kerekes, K., Farkas, Z., Giacometti, F., Canever, A., and Ambrus, \u00c1. (2019). Occurrence of aflatoxin M1 in raw milk marketed in Italy: Exposure assessment and risk characterization. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02516"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1002\/etc.4046","article-title":"Toward improved analysis of concentration data: Embracing nondetects","volume":"37","author":"Shoari","year":"2018","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1039\/D5EA00005J","article-title":"A novel statistical approach for analyzing environmental pollutant data with detection limits: Atmospheric organochloride pesticide concentrations near Tibet\u2019s Namco Lake as a case study","volume":"5","author":"Huang","year":"2025","journal-title":"Environ. Sci. Atmos."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3920\/WMJ2020.2672","article-title":"Aflatoxin B1 contamination of feedstuffs on a dairy farm in Northern Peru and aflatoxin M1 concentrations in raw milk","volume":"14","author":"Salazar","year":"2021","journal-title":"World Mycotoxin J."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Bervis, N., Lor\u00e1n, S., Juan, T., Carrami\u00f1ana, J.J., Herrera, A., Ari\u00f1o, A., and Herrera, M. (2021). Field monitoring of aflatoxins in feed and milk of high-yielding dairy cows under two feeding systems. Toxins, 13.","DOI":"10.3390\/toxins13030201"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"\u00c1lvarez-D\u00edas, F., Torres-Parga, B., Valdivia-Flores, A.G., Quezada-Trist\u00e1n, T., Alejos-De La Fuente, J.I., Sosa-Ram\u00edrez, J., and Rangel-Mu\u00f1oz, E.J. (2022). Aspergillus flavus and total aflatoxins occurrence in dairy feed and aflatoxin M1 in bovine milk in Aguascalientes, Mexico. Toxins, 14.","DOI":"10.3390\/toxins14050292"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Puga-Torres, B., Salazar, D., Cachiguango, M., Cisneros, G., and G\u00f3mez-Bravo, C. (2020). Determination of aflatoxin M1 in raw milk from different provinces of Ecuador. Toxins, 12.","DOI":"10.3390\/toxins12080498"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1080\/15569540903092027","article-title":"Preharvest aflatoxin contamination of corn and other grain crops grown on the US Southeastern Coastal Plain","volume":"28","author":"Scully","year":"2009","journal-title":"Toxin Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"85","DOI":"10.2520\/myco.67_2_3","article-title":"Feed and feed storage factors in relation to aflatoxin M1 contamination in bulk milk of smallholder dairy farms","volume":"67","author":"Chaisri","year":"2017","journal-title":"JSM Mycotoxins"},{"key":"ref_43","first-page":"163","article-title":"Carry-over of aflatoxin from cow\u2019s food to milk","volume":"55","author":"Veldman","year":"1992","journal-title":"Anim. Prod."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zentai, A., J\u00f3\u017awiak, \u00c1., S\u00fcth, M., and Farkas, Z. (2023). Carry-over of aflatoxin B1 from feed to cow milk-a review. Toxins, 15.","DOI":"10.3390\/toxins15030195"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1017\/S1751731107000663","article-title":"Carryover of aflatoxin from feed to milk in dairy cows with low or high somatic cell counts","volume":"1","author":"Masoero","year":"2007","journal-title":"Animal"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1021\/acs.jafc.2c08217","article-title":"Efficacy of bentonite and fumonisin esterase in mitigating the effects of aflatoxins and fumonisins in two Kenyan cattle breeds","volume":"71","author":"Kemboi","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Cha, M., Wang, E., Hao, Y., Ji, S., Huang, S., Zhao, L., Wang, W., Shao, W., Wang, Y., and Li, S. (2021). Adsorbents reduce aflatoxin M1 residue in milk of healthy dairy cow exposed to moderate level Aflatoxin B1 in diet and its exposure risk for humans. Toxins, 13.","DOI":"10.3390\/toxins13090665"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s12550-024-00576-9","article-title":"On-farm evaluation of aflatoxin binder inclusion in dairy feed as a strategy to reduce milk aflatoxin M1 contamination in the Rwandan context","volume":"41","author":"Nishimwe","year":"2025","journal-title":"Mycotoxin Res."},{"key":"ref_49","first-page":"1327","article-title":"Interlaboratory study of the Charm ROSA Safe Level Aflatoxin M1 Quantitative lateral flow test for raw bovine milk","volume":"89","author":"Salter","year":"2006","journal-title":"J. AOAC Int."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1080\/19440049.2014.979888","article-title":"Validation of a lateral flow test (MRLAFMQ) for the detection of aflatoxin M1 at 50 ng l\u22121 in raw commingled milk","volume":"31","author":"Reybroeck","year":"2014","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1093\/jaoacint\/qsaa175","article-title":"A lateral flow method for aflatoxin B1 in dry dog food: An inter-laboratory trial","volume":"104","author":"Vudathala","year":"2021","journal-title":"J. AOAC Int."},{"key":"ref_52","unstructured":"European Commission (EC) (2023). Commission implementing regulation (EU) 2023\/2782 of 14 December 2023 laying down the methods of sampling and analysis for the control of the levels of mycotoxins in food and repealing Regulation (EC) No 401\/2006. Off. J. Eur. Union, 1\u201344. Available online: http:\/\/data.europa.eu\/eli\/reg_impl\/2023\/2782\/oj."},{"key":"ref_53","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"Armbruster","year":"2008","journal-title":"Clin. Biochem. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"ujae023","DOI":"10.1093\/biomtc\/ujae023","article-title":"Behavioral carry-over effect and power consideration in crossover trials","volume":"80","author":"Shi","year":"2024","journal-title":"Biometrics"}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/18\/9\/386\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:20:37Z","timestamp":1788855637000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/18\/9\/386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":54,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["toxins18090386"],"URL":"https:\/\/doi.org\/10.3390\/toxins18090386","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,7]]}}}