{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:38:07Z","timestamp":1760243887002,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T00:00:00Z","timestamp":1268092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>DNA ploidy measurement has been applied uniquely to wax-embedded tissue of primary renal cell and metastatic tumours of a key experimental researcher on porcine ochratoxicosis, a control, and four transitional cell carcinomas from cases of Balkan endemic nephropathy. Primary renal tumour was diploid, and hyperdiploid metastasis was within the lower ploidy range for typical renal cell carcinoma. Three Balkan primary tumours showed extensive aneuploidy indicating marked nuclear instability, similar to model rat renal carcinoma caused by ochratoxin A. In contrast, much less nuclear instability in the putative occupational ochratoxicosis case fitted poorly with the ochratoxin A model.<\/jats:p>","DOI":"10.3390\/toxins2030326","type":"journal-article","created":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T11:09:10Z","timestamp":1268132950000},"page":"326-340","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Pilot Study of Nuclear Instability in Archived Renal and Upper Urinary Tract Tumours with Putative Ochratoxin Aetiology"],"prefix":"10.3390","volume":"2","author":[{"given":"Peter G.","family":"Mantle","sequence":"first","affiliation":[{"name":"Centre for Environmental Policy, Imperial College London, London SW7 2AZ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyrille","family":"Amerasinghe","sequence":"additional","affiliation":[{"name":"Department of Histopathology, Ealing Hospital, Southall, Middlesex, UB1 3HW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy L.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, Kings College London, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Herman","sequence":"additional","affiliation":[{"name":"Pathology Department, County Hospital Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Horn","sequence":"additional","affiliation":[{"name":"Pathology Department, Herlev Hospital, University of Copenhagen, DK-2730 Herlev, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thoger","family":"Krogh","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Aarhus University Hospital, 8000 Aarhus. Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward W.","family":"Odell","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, Kings College London, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Rosenbaum","sequence":"additional","affiliation":[{"name":"Department of Urology, Ealing Hospital, Southall, Middlesex. UB1 3HW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Calin A.","family":"Tatu","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Medicine and Pharmacy, Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2010,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1038\/2051112a0","article-title":"Ochratoxin A, a toxicmetabolite produced by Aspergillus ochraceus Wilh","volume":"205","author":"Steyn","year":"1965","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"van der Merwe, K.J., Steyn, P.S., and Fourie, L. (1965). Mycotoxins II. The constitution of ochratoxins A, B, and C, metabolites of Aspergillus ochraceus Wilh. J. Chem. Soc. Perkin 1, 7083\u20137088.","DOI":"10.1039\/jr9650007083"},{"key":"ref_3","first-page":"1","article-title":"Causal associations of mycotoxic nephropathy","volume":"Suppl.269","author":"Krogh","year":"1978","journal-title":"Acta. Pathol. Microbiol. Scand. Sect A"},{"key":"ref_4","unstructured":"Smith, J.E., and Henderson, R.S. (1991). Mycotoxins and Animal Foods, CRC Press."},{"key":"ref_5","first-page":"14","article-title":"Studies on the nephritides in the District of Vratza (in Bulgarian)","volume":"7","author":"Tanchev","year":"1956","journal-title":"Sovrem. Med."},{"key":"ref_6","first-page":"21","article-title":"The first clinical description of Balkan endemic nephropathy (1956) and its validity 35 years later","volume":"Volume 115","author":"Tanchev","year":"1991","journal-title":"Mycotoxins, Endemic Nephropathy and Urinary Tract Tumours"},{"key":"ref_7","unstructured":"WHO (1965). Memoranda. The endemic nephropathy of South-Eastern Europe."},{"key":"ref_8","unstructured":"Wolstenholme, G.E.W., and Knight, J. (1967). The Balkan Nephropathy. , J. and A. Churchill."},{"key":"ref_9","first-page":"219","article-title":"Balkan Nephropathy","volume":"68","author":"Austwick","year":"1975","journal-title":"Proc. R. Soc. Med."},{"key":"ref_10","unstructured":"Krogh, P. (1974, January 9\u201312). Mycotoxic porcine nephropathy- a possible model for Balkan (endemic) nephropathy. In Endemic nephropathy. Endemic nephropathy, Proceedings of the second International Symposium on Endemic Nephropathy."},{"key":"ref_11","first-page":"689","article-title":"Occurrence of ochratoxin A and citrinin in cereals associated with mycotoxic porcine nephropathy","volume":"81","author":"Krogh","year":"1973","journal-title":"Acta Pathol. Microbiol. Scand. Sect. A"},{"key":"ref_12","first-page":"1","article-title":"Experimental porcine nephropathy: Changes of renal function and structure induced by ochratoxin A-contaminated feed","volume":"246","author":"Krogh","year":"1974","journal-title":"Acta. Pathol. Microbiol. Scand. Sect. A Suppl."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/S0953-7562(09)80812-5","article-title":"Nephrotoxic fungi in a Yugoslavian community in which Balkan nephropathy is hyperendemic","volume":"95","author":"MacGeorge","year":"1991","journal-title":"Mycol. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0953-7562(09)80242-6","article-title":"Nephrotoxic fungi in foods from nephropathy households in Bulgaria","volume":"97","author":"Mantle","year":"1993","journal-title":"Mycol. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/BF00446997","article-title":"Ochratoxinogenicity of Aspergillus ochraceus strains from nephropathic and non-nephropathic areas in Yugoslavia","volume":"110","author":"Cvetnic","year":"1990","journal-title":"Mycopathologia"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1136\/vr.142.8.190","article-title":"Porcine nephropathy in Bulgaria: A progressive syndrome of complex or uncertain (mycotoxin) aetiology","volume":"142","author":"Stoev","year":"1998","journal-title":"Vet. Rec."},{"key":"ref_17","unstructured":"Boorman, G.A. (1989). Toxicology and Carcinogenesis studies of ochratoxin A, Toxicology and Carcinogenesis studies of ochratoxin A. NIH Publication No 89\u20132813 Research Triangle Park, NC, National Toxicology Programme."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1080\/10408440490265210","article-title":"Chemically induced renal tubule tumours in the laboratory rat and mouse: Review of the NCI\/NTP database and categorization of renal carcinogens based on mechanistic information","volume":"34","author":"Lock","year":"2004","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ijc.2910190102","article-title":"Geographic correlation between the occurrence of endemic nephropathy and urinary tract tumours in Vratza district","volume":"19","author":"Chernozemsky","year":"1977","journal-title":"Int.J. Cancer"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1080\/02652030500358431","article-title":"Renal tumourigenesis in male rats in response to chronic dietary ochratoxin A","volume":"1","author":"Mantle","year":"2005","journal-title":"Food Add. Contam."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.etp.2007.05.001","article-title":"DNA ploidy distribution in renal tumours induced in male rats by dietary ochratoxin A","volume":"59","author":"Brown","year":"2007","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.oraloncology.2004.10.009","article-title":"Heterogeneity, histological features and DNA ploidy in oral carcinoma by image-based analysis","volume":"41","author":"Diwakar","year":"2005","journal-title":"Oral Oncol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0091-679X(08)61721-5","article-title":"DNA analysis from paraffin-embedded blocks","volume":"41","author":"Hedley","year":"1994","journal-title":"Methods Cell Biol."},{"key":"ref_24","first-page":"7","article-title":"Metastatic Renal Cell Carcinoma: Novel therapies and health economics","volume":"13","author":"Sherwood","year":"2009","journal-title":"Urology News"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1177\/172460080502000408","article-title":"Prognostic biomarkers in renal cell carcinoma; relevance of DNA ploidy in predicting disease-related survival","volume":"20","author":"Pinto","year":"2005","journal-title":"Int. J. Biol. Markers"},{"key":"ref_26","first-page":"341","article-title":"The in vivo DNA aneuploidisation during expansion of conventional renal cell carcinoma","volume":"16","author":"Li","year":"2002","journal-title":"In Vivo"},{"key":"ref_27","first-page":"51","article-title":"Different DNA ploidy patterns for the differentiation of common subtypes of renal tumours","volume":"27","author":"Li","year":"2005","journal-title":"Cell. Oncol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.eururo.2004.03.020","article-title":"Prognostic value of microscopic venous invasion in renal cell carcinoma: long-term follow-up","volume":"46","author":"Lang","year":"2004","journal-title":"Eur. Urol."},{"key":"ref_29","unstructured":"Aitchison, M., Oliver, R.T.D., and Miles, A. (2004). The Effective Management of Renal Cell Carcinoma, Aesculapius Medical Press."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1093\/toxsci\/kfj017","article-title":"A toxicogenomics approach to identifying new plausible epigenetic mechanisms of ochratoxin A carcinogenicity in rat","volume":"89","author":"Nestler","year":"2006","journal-title":"Toxicol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1093\/toxsci\/kfl169","article-title":"Reduction in antioxidant defenses may contribute to ochratoxin a toxicity and carcinogenicity","volume":"96","author":"Cavin","year":"2007","journal-title":"Toxicol. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1021\/tx049650x","article-title":"Ochratoxin A causes DNA-damage and cytogenetic effects, but no DNA-adducts in rats","volume":"18","author":"Mally","year":"2005","journal-title":"Chem. Res. Toxicol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1002\/mnfr.200800149","article-title":"Mycotoxins and the kidney; Modes of action for renal tumor formation by ochratoxin A in rodents","volume":"53","author":"Mally","year":"2009","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_34","unstructured":"(2006). EFSA Opinion of the scientific panel on contaminants in the food chain on a request from the Commission related to ochratoxin A in food. EFSA J., 365, 1\u201356."},{"key":"ref_35","unstructured":"IARC Ochratoxin A (1993). IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 56, IARC Press."},{"key":"ref_36","unstructured":"Scudamore., K. (1993). Occurrence and Significance of Mycotoxins, Central Science Laboratory."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0378-4274(97)03877-0","article-title":"Ochratoxin A in human sera in the area with endemic nephropathy in Croatia","volume":"91","author":"Fuchs","year":"1997","journal-title":"Toxicol. Lett."},{"key":"ref_38","first-page":"141","article-title":"Ochratoxin A-related DNA adducts in urinary tract tumours of Bulgarian subjects","volume":"124","author":"Grosse","year":"1993","journal-title":"IARC Sci. Publ."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1002\/mnfr.200700045","article-title":"New molecular and field evidences for the implication of mycotoxins but not aristolochic acid in human nephropathy and urinary tract tumor","volume":"51","author":"Tozlovanu","year":"2007","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_40","first-page":"20","article-title":"Upper tract urothelial tumours","volume":"11","author":"Turney","year":"2007","journal-title":"Urology News"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1021\/jf990891y","article-title":"Co-occurrence of ochratoxin A and citrinin in cereals from Bulgarian villages with a history of Balkan Endemic Nephropathy","volume":"48","author":"Vrabcheva","year":"2000","journal-title":"J. Agric. FoodChem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/02652030500410315","article-title":"Biomarkers of human exposure to ochratoxin A","volume":"1","author":"Scott","year":"2005","journal-title":"Food Add. Contam."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2404","DOI":"10.1021\/jf030498z","article-title":"Analysis of ochratoxin A in foods consumed by inhabitants from an area with Balkan endemic nephropathy: A 1 month follow-up study","volume":"52","author":"Vrabcheva","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1080\/02652030210145036","article-title":"Ochratoxin A concentrations in food and feed from a region with Balkan endemic nephropathy","volume":"19","author":"Abouzeid","year":"2002","journal-title":"Food Add. Contam."},{"key":"ref_45","unstructured":"Pitt, J.I. (1979). The Genus Penicillium and Its Teleomorphic States Eupenicillium and Talaromyces, Academic Press."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/2\/3\/326\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:01:41Z","timestamp":1760220101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/2\/3\/326"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,9]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2010,3]]}},"alternative-id":["toxins2030326"],"URL":"https:\/\/doi.org\/10.3390\/toxins2030326","relation":{},"ISSN":["2072-6651"],"issn-type":[{"type":"electronic","value":"2072-6651"}],"subject":[],"published":{"date-parts":[[2010,3,9]]}}}