{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:34:49Z","timestamp":1760243689844,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2013,12,6]],"date-time":"2013-12-06T00:00:00Z","timestamp":1386288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The neurotoxic effects produced by a tentacle venom extract and a fraction were analyzed and correlated by in vivo and in vitro approaches. The tentacle venom extract exhibited a wide range of protein components (from 24 to &gt;225 kDa) and produced tetanic reactions, flaccid paralysis, and death when injected into crabs. Two chromatography fractions also produced uncontrolled appendix movements and leg stretching. Further electrophysiological characterization demonstrated that one of these fractions potently inhibited ACh-elicited currents mediated by both vertebrate fetal and adult muscle nicotinic acetylcholine receptors (nAChR) subtypes. Receptor inhibition was concentration-dependent and completely reversible. The calculated IC50 values were 1.77 \u03bcg\/\u03bcL for fetal and 2.28 \u03bcg\/\u03bcL for adult muscle nAChRs. The bioactive fraction was composed of a major protein component at ~90 kDa and lacked phospholipase A activity. This work represents the first insight into the interaction of jellyfish venom components and muscle nicotinic receptors.<\/jats:p>","DOI":"10.3390\/toxins5122420","type":"journal-article","created":{"date-parts":[[2013,12,6]],"date-time":"2013-12-06T12:12:35Z","timestamp":1386331955000},"page":"2420-2433","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita"],"prefix":"10.3390","volume":"5","author":[{"given":"Dalia","family":"Ponce","sequence":"first","affiliation":[{"name":"Instituto de Ciencias del Mar y Limnolog\u00eda, Unidad Acad\u00e9mica Puerto Morelos, Universidad Nacional Aut\u00f3noma de M\u00e9xico,77500 Canc\u00fan, Quintana Roo, Mexico"},{"name":"Instituto de Ciencias del Mar y Limnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Circuito Exterior s\/n, Ciudad Universitaria, 04510 Coyoac\u00e1n, Distrito Federal, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estuardo","family":"L\u00f3pez-Vera","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias del Mar y Limnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Circuito Exterior s\/n, Ciudad Universitaria, 04510 Coyoac\u00e1n, Distrito Federal, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Laboratorio de Neurofarmacolog\u00eda Marina, Departamento de Neurobiolog\u00eda Celular y Molecular, Instituto de Neurobiolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Campus Juriquilla, Juriquilla 76230, Quer\u00e9taro, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"S\u00e1nchez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias del Mar y Limnolog\u00eda, Unidad Acad\u00e9mica Puerto Morelos, Universidad Nacional Aut\u00f3noma de M\u00e9xico,77500 Canc\u00fan, Quintana Roo, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.3390\/md8041122","article-title":"Mediterranean jellyfish venoms: A review on scyphomedusae","volume":"8","author":"Mariottini","year":"2010","journal-title":"Mar. 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