{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T13:27:51Z","timestamp":1773408471440,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2014,3,5]],"date-time":"2014-03-05T00:00:00Z","timestamp":1393977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Selenocosmia jiafu is a medium-sized theraphosid spider and an attractive source of venom, because it can be bred in captivity and it produces large amounts of venom. We performed reversed-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser-desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses and showed that S. jiafu venom contains hundreds of peptides with a predominant mass of 3000\u20134500 Da. Patch clamp analyses indicated that the venom could inhibit voltage-gated Na+, K+ and Ca2+ channels in rat dorsal root ganglion (DRG) neurons. The venom exhibited inhibitory effects on tetrodotoxin-resistant (TTX-R) Na+ currents and T-type Ca2+ currents, suggesting the presence of antagonists to both channel types and providing a valuable tool for the investigation of these channels and for drug development. Intra-abdominal injection of the venom had severe toxic effects on cockroaches and caused death at higher concentrations. The LD50 was 84.24 \u03bcg\/g of body weight in the cockroach. However, no visible symptoms or behavioral changes were detected after intraperitoneal injection of the venom into mice even at doses up to 10 mg\/kg body weight. Our results provide a basis for further case-by-case investigations of peptide toxins from this venom.<\/jats:p>","DOI":"10.3390\/toxins6030988","type":"journal-article","created":{"date-parts":[[2014,3,5]],"date-time":"2014-03-05T11:48:39Z","timestamp":1394020119000},"page":"988-1001","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting on Voltage-Gated Ion Channels in Rat Dorsal Root Ganglion Neurons"],"prefix":"10.3390","volume":"6","author":[{"given":"Zhaotun","family":"Hu","sequence":"first","affiliation":[{"name":"Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China"},{"name":"Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Department of Life Science, Huaihua College, Huaihua, Hunan 418008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Xiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Department of Life Science, Huaihua College, Huaihua, Hunan 418008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dazhong","family":"Ying","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhonghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songping","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1146\/annurev.es.22.110191.003025","article-title":"Systematics and evolution of spiders (Araneae)","volume":"22","author":"Coddington","year":"1991","journal-title":"Annu. Rev. Ecol. Syst."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.3390\/toxins2122851","article-title":"Spider-venom peptides as therapeutics","volume":"12","author":"Saez","year":"2010","journal-title":"Toxins"},{"key":"ref_3","unstructured":"Platnick, N.I. The World Spider Catalog, Version 13.0; American Museum of Natural History. Available online:http:\/\/www.research.amnh.org\/iz\/spiders\/catalog."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nentwig, W. (1987). Ecophysiology of Spiders, Springer-Verlag.","DOI":"10.1007\/978-3-642-71552-5"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1636\/CA07-94.1","article-title":"Revision of the theraphosid spiders from China (Araneae: Mygalomorphae)","volume":"36","author":"Zhu","year":"2008","journal-title":"J. Arachnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.toxicon.2012.04.337","article-title":"Spider-venom peptides that target voltage-gated sodium channels: Pharmacological tools and potential therapeutic leads","volume":"60","author":"Klint","year":"2012","journal-title":"Toxicon"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3390\/toxins4030191","article-title":"Spider-venom peptides as bioinsecticides","volume":"4","author":"Windley","year":"2012","journal-title":"Toxins"},{"key":"ref_8","unstructured":"Kuhn-Nentwig, L., St\u00f6cklin, R., and Nentwig, W. (2011). Advances in Insect Physiology, Academic Press."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.toxicon.2006.01.018","article-title":"Venom landscapes: Mining the complexity of spider venoms via a combined cDNA and mass spectrometric approach","volume":"47","author":"Escoubas","year":"2006","journal-title":"Toxicon"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2792","DOI":"10.1021\/pr0700192","article-title":"Proteomic and peptidomic characterization of the venom from the Chinese bird spider, Ornithoctonus huwena Wang","volume":"6","author":"Yuan","year":"2007","journal-title":"J. Proteome Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1021\/pr1000016","article-title":"Molecular diversification of peptide toxins from the tarantula Haplopelma hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic, and genomic analyses","volume":"9","author":"Tang","year":"2010","journal-title":"J. Proteome Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1002\/pmic.200600785","article-title":"Proteomic and peptidomic analysis of the venom from Chinese tarantula Chilobrachys jingzhao","volume":"7","author":"Liao","year":"2007","journal-title":"Proteomics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S1474-4422(10)70059-4","article-title":"Voltage-gated sodium channels as therapeutic targets in epilepsy and other neurological disorders","volume":"9","author":"Mantegazza","year":"2010","journal-title":"Lancet Neurol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1113\/jphysiol.1993.sp019706","article-title":"Kinetic analysis of two types of Na+ channels in rat dorsal root ganglia","volume":"466","author":"Ogata","year":"1993","journal-title":"J. Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.mcn.2010.08.017","article-title":"Single-cell analysis of sodium channel expression in dorsal root ganglion neurons","volume":"46","author":"Ho","year":"2011","journal-title":"Mol. Cell. Neurosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s11916-009-0034-9","article-title":"Contribution of primary afferent channels to neuropathic pain","volume":"13","author":"Harriott","year":"2009","journal-title":"Curr. Pain Headache Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"293","DOI":"10.7150\/ijbs.5.293","article-title":"Characterization of persistent TTX-R Na+  currents in physiological concentration of sodium in rat visceral afferents","volume":"5","author":"Qiao","year":"2009","journal-title":"Int. J. Biol. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"13373","DOI":"10.1073\/pnas.231376298","article-title":"Distinct potassium channels on pain-sensing neurons","volume":"98","author":"Rasband","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Takeda, M., Tsuboi, Y., Kitagawa, J., Nakagawa, K., Iwata, K., and Matsumoto, S. (2011). Potassium channels as a potential therapeutic target for trigeminal neuropathic and inflammatory pain. Mol. Pain, 7.","DOI":"10.1186\/1744-8069-7-5"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/S0959-4388(96)80117-6","article-title":"Presynaptic potassium channels","volume":"6","author":"Roeper","year":"1996","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Catterall, W.A. (2011). Voltage-gated calcium channels. Cold Spring Harb. Perspect. Biol., 3.","DOI":"10.1101\/cshperspect.a003947"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1146\/annurev.ne.17.030194.002151","article-title":"Molecular basis for Ca2+ channel diversity","volume":"17","author":"Hofmann","year":"1994","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1146\/annurev.bi.63.070194.004135","article-title":"Calcium channel diversity and neurotransmitter release: The omega-conotoxins and omega-agatoxins","volume":"63","author":"Olivera","year":"1994","journal-title":"Annu. Rev. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.2174\/138161206777585184","article-title":"The role of T-type calcium channels in epilepsy and pain","volume":"12","author":"Nelson","year":"2006","journal-title":"Curr. Pharm. Des."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jpain.2005.09.007","article-title":"Calcium channels as therapeutic targets in neuropathic pain","volume":"7","author":"Yaksh","year":"2006","journal-title":"J. Pain"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.pain.2006.10.019","article-title":"Voltage-gated calcium channels and pain","volume":"126","author":"Cao","year":"2006","journal-title":"Pain"},{"key":"ref_27","first-page":"60","article-title":"Biological characterization of spider (Selenocosmia huwena) crude venom","volume":"14","author":"Liang","year":"1993","journal-title":"Zool. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.toxicon.2004.02.005","article-title":"An overview of peptide toxins from the venom of the Chinese bird spider Selenocosmia huwena Wang [=Ornithoctonus huwena (Wang)]","volume":"43","author":"Liang","year":"2004","journal-title":"Toxicon"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8628","DOI":"10.1074\/jbc.M513542200","article-title":"Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena","volume":"281","author":"Liu","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_30","first-page":"82","article-title":"Inhibition of sodium channels in rat dorsal root ganglion neurons by hainantoxin-IV, a novel spider toxin","volume":"35","author":"Xiao","year":"2003","journal-title":"Acta Biochim. Biophys. Sin."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/6\/3\/988\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:08:56Z","timestamp":1760216936000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/6\/3\/988"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,5]]},"references-count":30,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,3]]}},"alternative-id":["toxins6030988"],"URL":"https:\/\/doi.org\/10.3390\/toxins6030988","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,5]]}}}