{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T11:05:37Z","timestamp":1783076737788,"version":"3.54.6"},"reference-count":32,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T00:00:00Z","timestamp":1438128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["ES03553"],"award-info":[{"award-number":["ES03553"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"USDA","doi-asserted-by":"publisher","award":["59-0206-9-058"],"award-info":[{"award-number":["59-0206-9-058"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"name":"USDA-NIFA","award":["2011-0635"],"award-info":[{"award-number":["2011-0635"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Deoxynivalenol (DON, vomitoxin), a common trichothecene mycotoxin found in cereal foods, dysregulates immune function and maintenance of energy balance. The purpose of this study was to determine if sex differences are similarly evident in DON\u2019s anorectic responses in mice. A bioassay for feed refusal, previously developed by our lab, was used to compare acute i.p. exposures of 1 and 5 mg\/kg bw DON in C57BL6 mice. Greater anorectic responses were seen in male than female mice. Male mice had higher organ and plasma concentrations of DON upon acute exposure than their female counterparts. A significant increase in IL-6 plasma levels was also observed in males while cholecystokinin response was higher in females. When effects of sex on food intake and body weight changes were compared after subchronic dietary exposure to 1, 2.5, and 10 ppm DON, males were found again to be more sensitive. Demonstration of male predilection to DON-induced changes in food intake and weight gain might an important consideration in future risk assessment of DON and other trichothecenes.<\/jats:p>","DOI":"10.3390\/toxins7082845","type":"journal-article","created":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T10:25:31Z","timestamp":1438165531000},"page":"2845-2859","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Murine Anorectic Response to Deoxynivalenol (Vomitoxin) Is Sex-Dependent"],"prefix":"10.3390","volume":"7","author":[{"given":"Erica","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenna","family":"Flannery","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Pestka","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s00204-010-0579-8","article-title":"Deoxynivalenol: Mechanisms of action, human exposure, and toxicological relevance","volume":"84","author":"Pestka","year":"2010","journal-title":"Arch. Toxicol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"663","DOI":"10.3390\/toxins4090663","article-title":"A three-year survey on the worldwide occurence of mycotoxins in feedstuffs and feed","volume":"4","author":"Rodrigues","year":"2012","journal-title":"Toxins"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"527","DOI":"10.2460\/javma.1984.185.05.527","article-title":"Feeding trials with vomitoxin (deoxynivalenol)-contaminated wheat\u2014effects on swine, poultry, and dairy-cattle","volume":"185","author":"Trenholm","year":"1984","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/tcm.1770150606","article-title":"Chronic feeding study of deoxynivalenol in B6C3F1 male and female mice","volume":"15","author":"Iverson","year":"1995","journal-title":"Teratog. Carcinog. Mutagen."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/toxsci\/kfp225","article-title":"Suppression of insulin-like growth factor acid-labile subunit expression-a novel mechanism for deoxynivalenol-induced growth retardation","volume":"113","author":"Amuzie","year":"2010","journal-title":"Toxicol. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1093\/toxsci\/kfp287","article-title":"A new perspective on deoxynivalenol and growth suppression","volume":"113","author":"Voss","year":"2010","journal-title":"Toxicol. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1093\/toxsci\/kfs286","article-title":"Comparison of emetic potencies of the 8-ketotrichothecenes deoxynivalenol, 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, fusarenon x, and nivalenol","volume":"131","author":"Wu","year":"2013","journal-title":"Toxicol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1128\/aem.34.5.547-552.1977","article-title":"Emetic and refusal activity of deoxynivalenol to swine","volume":"34","author":"Forsyth","year":"1977","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0278-6915(86)90231-0","article-title":"Effects of 8-week exposure of the B6C3F1 mouse to dietary deoxynivalenol (vomitoxin) and zearalenone","volume":"24","author":"Forsell","year":"1986","journal-title":"Food Chem. Toxicol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1016\/j.fct.2011.05.004","article-title":"Characterization of deoxynivalenol-induced anorexia using mouse bioassay","volume":"49","author":"Flannery","year":"2011","journal-title":"Food Chem. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1080\/15287399409531902","article-title":"Role of gender and strain in vomitoxin-induced dsyregulation of iga production and iga nephropathy in the mouse","volume":"43","author":"Greene","year":"1994","journal-title":"J. Toxicol. Environ. Health"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"942","DOI":"10.2527\/jas1985.614942x","article-title":"Sex-related reduced weight gains in growing swine fed diets containing deoxynivalenol","volume":"61","author":"Cote","year":"1985","journal-title":"J. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1007\/BF00196934","article-title":"Optimization of the mouse bioassay for deoxynivalenol as an alternative to large animal studies","volume":"53","author":"Rotter","year":"1994","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0300-483X(94)90181-3","article-title":"Vomitoxin (deoxynivalenol)-induced iga nephropathy in the B6C3F1 mouse - dose-response and male predilection","volume":"92","author":"Greene","year":"1994","journal-title":"Toxicology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1017\/S1751731111002278","article-title":"Meta-analytical study of productive and nutritional interactions of mycotoxins in growing pigs","volume":"6","author":"Andretta","year":"2012","journal-title":"Animal"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1006\/faat.1995.1080","article-title":"Effects of dihydrotestosterone and estradiol on experimental IgA nephropathy induced by vomitoxin","volume":"26","author":"Greene","year":"1995","journal-title":"Fundam. Appl. Toxicol."},{"key":"ref_17","first-page":"222","article-title":"Cytokine-induced sickness behavior: Mechanisms and implications","volume":"Volume 933","author":"Sorg","year":"2001","journal-title":"Role of Neural Plasticity in Chemical Intolerance"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.neuro.2015.04.009","article-title":"Dysregulation of energy balance by trichothecene mycotoxins: Mechanisms and prospects","volume":"49","author":"Lebrun","year":"2015","journal-title":"Neurotoxicology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/toxsci\/kfr219","article-title":"Central inflammation and sickness-like behavior induced by the food contaminant deoxynivalenol: A PGE2-independent mechanism","volume":"124","author":"Girardet","year":"2011","journal-title":"Toxicol. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1006\/taap.1995.1132","article-title":"Induction of cytokine messenger-RNAs in mice after oral-exposure to the trichothecene vomitoxin (deoxynivalenol)\u2014relationship to toxin distribution and protein-synthesis inhibition","volume":"133","author":"Ouyang","year":"1995","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.1016\/j.fct.2008.05.016","article-title":"Tissue distribution and proinflammatory cytokine gene expression following acute oral exposure to deoxynivalenol: Comparison of weanling and adult mice","volume":"46","author":"Pestka","year":"2008","journal-title":"Food Chem. Toxicol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1093\/toxsci\/kfs255","article-title":"Anorexia induction by the trichothecene deoxynivalenol (vomitoxin) is mediated by the release of the gut satiety hormone peptide yy","volume":"130","author":"Flannery","year":"2012","journal-title":"Toxicol. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1093\/toxsci\/kft335","article-title":"Role of cholecystokinin in anorexia induction following oral exposure to the 8-ketotrichothecenes deoxynivalenol, 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, fusarenon x, and nivalenol","volume":"138","author":"Wu","year":"2014","journal-title":"Toxicol. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.1523\/JNEUROSCI.5508-06.2007","article-title":"Cocaine- and amphetamine-regulated transcript: Stimulation of expression in rat vagal afferent neurons by cholecystokinin and suppression by ghrelin","volume":"27","author":"Dimaline","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.bbrc.2003.10.089","article-title":"Acute effects of PYY3\u201336 on food intake and hypothalamic neuropeptide expression in the mouse","volume":"311","author":"Challis","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.toxlet.2008.02.005","article-title":"Immunochemical assessment of deoxynivalenol tissue distribution following oral exposure in the mouse","volume":"178","author":"Pestka","year":"2008","journal-title":"Toxicol. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1021\/jf00004a026","article-title":"Tissue distribution of deoxynivalenol in swine dosed intravenously","volume":"39","author":"Prelusky","year":"1991","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1124\/dmd.106.012070","article-title":"Tissue- and gender-specific mrna expression of UDP-glucuronosyltransferases (UGTs) in mice","volume":"35","author":"Buckley","year":"2007","journal-title":"Drug Metab. Dispos."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1124\/dmd.108.024224","article-title":"Mechanism of gender-divergent UDP-glucuronosyltransferase mrna expression in mouse liver and kidney","volume":"37","author":"Buckley","year":"2009","journal-title":"Drug Metab. Dispos."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1093\/toxsci\/kfp150","article-title":"Induction of suppressors of cytokine signaling by the trichothecene deoxynivalenol in the mouse","volume":"111","author":"Amuzie","year":"2009","journal-title":"Toxicol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1093\/toxsci\/kfg119","article-title":"Role of IL-1 beta in endotoxin potentiation of deoxynivalenol-induced corticosterone response and leukocyte apoptosis in mice","volume":"74","author":"Islam","year":"2003","journal-title":"Toxicol. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s12550-013-0161-3","article-title":"Effects of oral deoxynivalenol exposure on immune-related parameters in lymphoid organs and serum of mice vaccinated with porcine parvovirus vaccine","volume":"29","author":"Choi","year":"2013","journal-title":"Mycotoxin Res."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/7\/8\/2845\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:49:52Z","timestamp":1760215792000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/7\/8\/2845"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,29]]},"references-count":32,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2015,8]]}},"alternative-id":["toxins7082845"],"URL":"https:\/\/doi.org\/10.3390\/toxins7082845","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,29]]}}}