{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:34:40Z","timestamp":1770341680356,"version":"3.49.0"},"reference-count":58,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2016,7,20]],"date-time":"2016-07-20T00:00:00Z","timestamp":1468972800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico - CNPq","award":["308183\/2009-6"],"award-info":[{"award-number":["308183\/2009-6"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais - FAPEMIG","award":["APQ-00412-09"],"award-info":[{"award-number":["APQ-00412-09"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior - CAPES","award":["AUXPE-PNPD-2314.2009"],"award-info":[{"award-number":["AUXPE-PNPD-2314.2009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Tumor necrosis factor (TNF) is a major cytokine in inflammatory processes and its deregulation plays a pivotal role in several diseases. Here, we report that a zinc metalloprotease extracted from Bothrops moojeni venom (BmooMP-alpha-I) inhibits TNF directly by promoting its degradation. This inhibition was demonstrated by both in vitro and in vivo assays, using known TLR ligands. These findings are supported by molecular docking results, which reveal interaction between BmooMP-alpha-I and TNF. The major cluster of interaction between BmooMP-alpha-I and TNF was confirmed by the structural alignment presenting Ligand Root Mean Square Deviation LRMS = 1.05 \u00c5 and Interactive Root Mean Square Deviation IRMS = 1.01 \u00c5, this result being compatible with an accurate complex. Additionally, we demonstrated that the effect of this metalloprotease on TNF is independent of cell cytotoxicity and it does not affect other TLR-triggered cytokines, such as IL-12. Together, these results indicate that this zinc metalloprotease is a potential tool to be further investigated for the treatment of inflammatory disorders involving TNF deregulation.<\/jats:p>","DOI":"10.3390\/toxins8070223","type":"journal-article","created":{"date-parts":[[2016,7,20]],"date-time":"2016-07-20T09:45:15Z","timestamp":1469007915000},"page":"223","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Interaction between TNF and BmooMP-Alpha-I, a Zinc Metalloprotease Derived from Bothrops moojeni Snake Venom, Promotes Direct Proteolysis of This Cytokine: Molecular Modeling and Docking at a Glance"],"prefix":"10.3390","volume":"8","author":[{"given":"Maraisa","family":"Silva","sequence":"first","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Immunoparasitology \u201cDr. Mario Endsfeldz Camargo\u201d, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamires","family":"Lopes Silva","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Immunoparasitology \u201cDr. Mario Endsfeldz Camargo\u201d, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murilo","family":"Silva","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Immunoparasitology \u201cDr. Mario Endsfeldz Camargo\u201d, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Mota","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Immunoparasitology \u201cDr. Mario Endsfeldz Camargo\u201d, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Santiago","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Immunoparasitology \u201cDr. Mario Endsfeldz Camargo\u201d, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Biophysics, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1bio","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Biophysics, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago","family":"Mineo","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Immunoparasitology \u201cDr. Mario Endsfeldz Camargo\u201d, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Mineo","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Laboratory of Immunoparasitology \u201cDr. Mario Endsfeldz Camargo\u201d, Federal University of Uberl\u00e2ndia, Av. Par\u00e1 1720, Uberl\u00e2ndia 38400-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Osta, B., Benedetti, G., and Miossec, P. (2014). Classical and paradoxical effects of TNF-alpha on bone homeostasis. Front. Immunol., 5.","DOI":"10.3389\/fimmu.2014.00048"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.smim.2014.02.009","article-title":"TNF and MAP kinase signalling pathways","volume":"26","author":"Sabio","year":"2014","journal-title":"Semin. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.semcdb.2015.10.014","article-title":"The cybernetics of TNF: Old views and newer ones","volume":"50","author":"Wallach","year":"2016","journal-title":"Semin. Cell. Dev. 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