{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:15:29Z","timestamp":1767374129019,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2016,12,13]],"date-time":"2016-12-13T00:00:00Z","timestamp":1481587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Snake venom is a complex mixture of proteins and peptides which in the Viperidae is mainly hemotoxic. The diversity of these components causes the venom to be an extremely interesting object of study. Discovered components can be used in search for new pharmaceuticals used primarily in the treatment of diseases of the cardiovascular system. In order to determine the protein composition of the southern copperhead venom, we have used high resolution two dimensional electrophoresis and MALDI ToF\/ToF MS-based identification. We have identified 10 groups of proteins present in the venom, of which phospholipase A2 and metalloprotease and serine proteases constitute the largest groups. For the first time presence of 5\u2032-nucleotidase in venom was found in this group of snakes. Three peptides present in the venom were also identified. Two of them as bradykinin-potentiating agents and one as an inhibitor.<\/jats:p>","DOI":"10.3390\/toxins8120372","type":"journal-article","created":{"date-parts":[[2016,12,13]],"date-time":"2016-12-13T10:15:52Z","timestamp":1481624152000},"page":"372","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques"],"prefix":"10.3390","volume":"8","author":[{"given":"Aleksandra","family":"Bocian","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u0142gorzata","family":"Urbanik","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2723-3691","authenticated-orcid":false,"given":"Konrad","family":"Hus","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8625-4164","authenticated-orcid":false,"given":"Andrzej","family":"\u0141yskowski","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladim\u00edr","family":"Petrilla","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovak Republic"},{"name":"Zoological Department, Zoological Garden Ko\u0161ice, \u0160irok\u00e1 31, 040 06 Ko\u0161ice-Kave\u010dany, Slovac Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuzana","family":"Andrej\u010d\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Petrillov\u00e1","sequence":"additional","affiliation":[{"name":"Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslav","family":"Leg\u00e1th","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"},{"name":"Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,13]]},"reference":[{"key":"ref_1","unstructured":"Campbell, J.A., and Lamar, W.W. 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