{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T00:00:46Z","timestamp":1794009646289,"version":"4.8.0"},"reference-count":26,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United States Agency for International Development (USAID)","award":["NTDSC 244G"],"award-info":[{"award-number":["NTDSC 244G"]}]},{"name":"United States Agency for International Development (USAID)","award":["APP 1193826"],"award-info":[{"award-number":["APP 1193826"]}]},{"name":"Australian National Health and Medical Research Council Fellowship","award":["NTDSC 244G"],"award-info":[{"award-number":["NTDSC 244G"]}]},{"name":"Australian National Health and Medical Research Council Fellowship","award":["APP 1193826"],"award-info":[{"award-number":["APP 1193826"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Accurate rapid diagnostic tests (RDTs) are needed to diagnose lymphatic filariasis (LF) in global elimination programmes. We evaluated the performance of the new STANDARD Q Filariasis Antigen Test (QFAT) against the Bioline Filariasis Test Strip (FTS) for detecting W. bancrofti antigen (Ag) in laboratory conditions, using serum (n = 195) and plasma (n = 189) from LF-endemic areas (Samoa, American Samoa and Myanmar) and Australian negative controls (n = 46). The prior Ag status of endemic samples (54.9% Ag-positive) was determined by rapid test (ICT or FTS) or Og4C3 ELISA. The proportion of samples testing positive at 10 min was similar for QFAT (44.8%) and FTS (41.3%). Concordance between tests was 93.5% (kappa 0.87, n = 417) at 10 min, and it increased to 98.8% (kappa 0.98) at 24 h. The sensitivities of QFAT and FTS at 10 min compared to the prior results were 92% (95% CI 88.0\u201396.0) and 86% (95% CI 80.0\u201390.0), respectively, and they increased to 97% and 99% at 24 h. Specificity was 98% for QFAT and 99% for FTS at 10 min. Both tests showed evidence of cross-reaction with Dirofilaria repens and Onchocerca lupi but not with Acanthochilonema reconditum or Cercopithifilaria bainae. Under laboratory conditions, QFAT is a suitable alternative RDT to FTS.<\/jats:p>","DOI":"10.3390\/tropicalmed10010023","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T07:35:47Z","timestamp":1736840147000},"page":"23","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Laboratory Comparison of Rapid Antigen Diagnostic Tests for Lymphatic Filariasis: STANDARD Q Filariasis Antigen Test (QFAT) Versus Bioline Filariasis Test Strip (FTS)"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5215-3901","authenticated-orcid":false,"given":"Patricia M.","family":"Graves","sequence":"first","affiliation":[{"name":"WHO Collaborating Centre for Vector-Borne and Neglected Tropical Diseases, College of Public Health, Medical and Veterinary Sciences and Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD 4878, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3210-6629","authenticated-orcid":false,"given":"Jessica L.","family":"Scott","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Vector-Borne and Neglected Tropical Diseases, College of Public Health, Medical and Veterinary Sciences and Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD 4878, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro","family":"Berg Soto","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Vector-Borne and Neglected Tropical Diseases, College of Public Health, Medical and Veterinary Sciences and Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD 4878, Australia"},{"name":"Andes Analytics, Santiago 7560408, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8397-3358","authenticated-orcid":false,"given":"Antin Y. N.","family":"Widi","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Vector-Borne and Neglected Tropical Diseases, College of Public Health, Medical and Veterinary Sciences and Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD 4878, Australia"},{"name":"Faculty of Medicine and Veterinary Medicine, Nusa Cendana University, Kupang 85228, East Nusa Tenggara, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1677-2991","authenticated-orcid":false,"given":"Maxine","family":"Whittaker","sequence":"additional","affiliation":[{"name":"College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD 4811, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8288-4169","authenticated-orcid":false,"given":"Colleen L.","family":"Lau","sequence":"additional","affiliation":[{"name":"School of Public Health, Faculty of Medicine, The University of Queensland, Brisbane, QLD 4006, Australia"},{"name":"Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Brisbane, QLD 4006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly Y.","family":"Won","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2024). 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