{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:08:36Z","timestamp":1793660916291,"version":"4.5.1"},"reference-count":112,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"North Shore Mosquito Abatement District"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>This systematic review and meta-analysis aims to: (1) characterize the distribution of published inhibition of emergence (IE50, IE90, and IE95) reference values for pyriproxyfen and methoprene in Culex pipiens [L.], Aedes aegypti [L.], and Aedes albopictus [Skuse]; (2) generate combined-effect IE values using a DerSimonian and Laird (DL) random-effects model to establish benchmarks for future resistance assessments; and (3) compare these combined-effect IE values with previously published literature. A systematic search was conducted in PubMed, SciELO, J-STAGE, and Google Scholar up to 10 February 2025, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Eligible studies were primary, peer-reviewed literature that aligned with World Health Organization (WHO) protocols for insect growth regulator (IGR) resistance testing, specifically those reporting susceptible reference IE values from continuous immersion dose-response bioassays analyzed using probit regression. A total of 72 unique studies that aligned with WHO protocols were assessed for publication bias using a funnel plot and Egger\u2019s regression. Sensitivity and subgroup analyses were conducted to evaluate individual study contributions to the overall combined effect. Heterogeneity (I2) and combined effect values were estimated for 18 different species\/active ingredient\/IE concentration subgroup pairings. Heterogeneity (I2) ranged from 29.32 to 99.78% between the 18 subgroups, indicating inconsistency within the literature. The DL combined effect IE50 varied from 0.048 ppb for Cx. pipiens exposed to pyriproxyfen to 1.818 ppb for Ae. albopictus exposed to methoprene. A certainty analysis indicated that 1 combined effect value exhibited high certainty, 8 out of 18 pairings were moderately certain, 6 exhibited low certainty and 3 exhibited very low certainty. The main causes of uncertainty (ranked) were inconsistency between studies, imprecision of the combined effect size, and possible publication bias. Our findings indicate that (1) robust DL combined effect IE50 values could be established for all species\/IGR pairings, providing essential benchmarks for future resistance assessments; (2) substantial heterogeneity among susceptible laboratory colonies complicates resistance detection in field-collected mosquitoes; and (3) a significant portion of the literature relies on reference mosquito strains that are likely not fully susceptible, further complicating resistance detection. This study was not registered and was supported by the North Shore Mosquito Abatement District.<\/jats:p>","DOI":"10.3390\/tropicalmed10040087","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T10:54:48Z","timestamp":1743159288000},"page":"87","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Assessing Insect Growth Regulator Resistance Using Bioassays: A Systematic Review and Meta-Analysis of Methoprene and Pyriproxyfen Inhibition of Emergence in Three Vector Mosquito Species"],"prefix":"10.3390","volume":"10","author":[{"given":"Mark E.","family":"Clifton","sequence":"first","affiliation":[{"name":"North Shore Mosquito Abatement District, 117 Northfield Rd, Northfield, IL 60093, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Lopez","sequence":"additional","affiliation":[{"name":"North Shore Mosquito Abatement District, 117 Northfield Rd, Northfield, IL 60093, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1016\/S0140-6736(12)61151-9","article-title":"Drivers, dynamics, and control of emerging vector-borne zoonotic diseases","volume":"380","author":"Kilpatrick","year":"2012","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1093\/jme\/tjz074","article-title":"The need for a national strategy to address vector-borne disease threats in the United States","volume":"56","author":"Beard","year":"2019","journal-title":"J. Med. Entomol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/s41579-024-01026-0","article-title":"Effects of climate change and human activities on vector-borne diseases","volume":"22","author":"Weaver","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Patterson, G. (2009). The Mosquito Crusades: A History of the American Anti-Mosquito Movement from the Reed Commission to the First Earth Day, Rutgers University Press.","DOI":"10.2307\/j.ctt5hj0gq"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Becker, N., Petric, D., Zgomba, M., Boase, C., Madon, M., Dahl, C., and Kaiser, A. (2010). Mosquitos and Their Control, Springer. [2nd ed.].","DOI":"10.1007\/978-3-540-92874-4"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"527","DOI":"10.2987\/8756-971X(2006)22[527:MLCPPA]2.0.CO;2","article-title":"Mosquito Larval Control Practices: Past and Present","volume":"22","author":"Floore","year":"2006","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Trdan, S. (2016). Resistance and Its Management to Microbial and Insect Growth Regulator Larvicides in Mosquitoes. Insecticide Resistance, InTechOpen.","DOI":"10.5772\/60478"},{"key":"ref_8","first-page":"9","article-title":"Resistance and resistance management of biorational larvicides for mosquito control","volume":"69","author":"Su","year":"2022","journal-title":"J. Florida Mosq. Control Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1603\/ME13173","article-title":"Laboratory selection of resistance to spinosad in Culex quinquefasciatus (Diptera: Culicidae)","volume":"51","author":"Su","year":"2014","journal-title":"J. Med. Entomol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Williams, C.M. (1967). Third-Generation Pesticides, Scientific American Magazine.","DOI":"10.1038\/scientificamerican0767-13"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Subramanyam, B., and Hagstrum, D.W. (2000). Insect Growth Regulators. Alternatives to Pesticides in Stored-Product IPM, Springer.","DOI":"10.1007\/978-1-4615-4353-4"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.ecoenv.2016.12.038","article-title":"Environmental safety review of methoprene and bacterially-derived pesticides commonly used for sustained mosquito control","volume":"139","author":"Lawler","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_13","unstructured":"World Health Organization (WHO) (2000). Review of the insect growth regulator pyriproxyfen. Report of the 4th WHOPES Working Group Meeting, WHO Press."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1584\/jpestics.D20-084","article-title":"Stage-specific action of juvenile hormone analogs","volume":"46","author":"Parthasarathy","year":"2021","journal-title":"J. Pestic. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.ibmb.2005.01.019","article-title":"Expression of nuclear receptor-transcription factor genes during Aedes aegypti midgut metamorphosis and the effect of methoprene on expression","volume":"35","author":"Nishiura","year":"2005","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.mod.2006.05.005","article-title":"Mechanisms of midgut remodeling: Juvenile hormone analog methoprene blocks midgut metamorphosis by modulating ecdysone action","volume":"123","author":"Wu","year":"2006","journal-title":"Mech. Dev."},{"key":"ref_17","unstructured":"World Health Organization (WHO) (2005). Guidelines for Laboratory and Field Testing of Mosquito Larvicides, WHO Press."},{"key":"ref_18","unstructured":"World Health Organization (WHO) (2016). Monitoring and Managing Insecticide Resistance in Aedes mosquito Populations: Interim Guidance for Entomologists, WHO Press."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e65655","DOI":"10.7554\/eLife.65655","article-title":"The need for practical insecticide-resistance guidelines to effectively inform mosquito-borne disease control programs","volume":"6","author":"Namias","year":"2021","journal-title":"Elife."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1093\/jme\/tjac076","article-title":"Survey of United States mosquito control programs reveals opportunities to improve the operational value of Centers for Disease Control and Prevention bottle bioassays","volume":"59","author":"Richards","year":"2022","journal-title":"J. Med. Entomol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.ijsu.2010.02.007","article-title":"Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement","volume":"8","author":"Moher","year":"2010","journal-title":"Int. J. Surg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e1230","DOI":"10.1002\/cl2.1230","article-title":"PRISMA2020: An R package and Shiny app for producing PRISMA 2020-compliant flow diagrams, with interactivity for optimized digital transparency and Open Synthesis","volume":"18","author":"Haddaway","year":"2022","journal-title":"Campbell Syst. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"A12-3","DOI":"10.7326\/ACPJC-1995-123-3-A12","article-title":"The well-built clinical question: A key to evidence-based decisions","volume":"123","author":"Richardson","year":"1995","journal-title":"ACP J. Club"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1136\/bmj.315.7109.629","article-title":"Bias in meta-analysis detected by a simple, graphical test","volume":"315","author":"Egger","year":"1997","journal-title":"BMJ"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Koricheva, J., Gurevitch, J., and Mengersen, K. (2013). Recovering missing or partial data from studies: A survey of conversions and imputations for meta-analysis. Handbook of Meta-Analysis in Ecology and Evolution, Princeton University Press.","DOI":"10.23943\/princeton\/9780691137285.001.0001"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1002\/jrsm.1260","article-title":"Introduction, comparison and validation of Meta-Essentials: A free and simple tool for meta-analysis","volume":"8","author":"Suurmond","year":"2017","journal-title":"Res. Synth. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0197-2456(86)90046-2","article-title":"Meta-analysis in clinical trials","volume":"7","author":"DerSimonian","year":"1986","journal-title":"Control Clin. Trials"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1037\/1082-989X.13.1.31","article-title":"Confidence intervals for the overall effect size in random-effects meta-analysis","volume":"13","year":"2008","journal-title":"Psychol. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Van Rhee, H.J., Suurmond, R., and Hak, T. (2015). User Manual for Meta-Essentials: Workbooks for Meta-Analysis (Version 1.4), Erasmus Research Institute of Management. Available online: www.erim.eur.nl\/research-support\/meta-essentials.","DOI":"10.2139\/ssrn.3241355"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Motulsky, H.J., and Brown, R.E. (2006). Detecting outliers when fitting data with nonlinear regression\u2013a new method based on robust nonlinear regression and the false discovery rate. BMC Bioinf., 7.","DOI":"10.1186\/1471-2105-7-123"},{"key":"ref_31","unstructured":"Sch\u00fcnemann, H., Bro\u017cek, J., Guyatt, G., and Oxman, A. (2025, February 02). GRADE Handbook for Grading Quality of Evidence and Strength of Recommendations. Available online: https:\/\/gdt.gradepro.org\/app\/handbook\/handbook.html."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1590\/S0074-02761992000400024","article-title":"Laboratory evaluation of three insect growth regulators against a malathion resistant strain of Culex quinquefasciatus (Diptera: Culicidae)","volume":"87","author":"Navarro","year":"1992","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_33","first-page":"30","article-title":"Comparative susceptibility of methoprene and organophosphorus insecticides in Japanese and Chinese populations of Culex pipiens","volume":"4","author":"Kamei","year":"1992","journal-title":"Jpn. J. Environ. Entomol. Zool."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1093\/jme\/tjx235","article-title":"Resistance to Lysinibacillus sphaericus and other commonly used pesticides in Culex pipiens (Diptera: Culicidae) from Chico, California","volume":"55","author":"Su","year":"2018","journal-title":"J. Med. Entomol."},{"key":"ref_35","first-page":"3197","article-title":"Oxime ethers: New potent insect growth regulators","volume":"49","author":"Ohsumi","year":"1985","journal-title":"Agric. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"185","DOI":"10.7601\/mez.25.185","article-title":"Difference in the susceptibility of Culex larvae against methoprene and its slow-release formulation A at various stages of development","volume":"25","author":"Noguchi","year":"1974","journal-title":"Jpn. J. Sanit. Zool."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"271","DOI":"10.7601\/mez.38.271","article-title":"Laboratory evaluation of 2-[1-methyl-2-(4-phenoxyphenoxy) ethoxy] pyridine against larvae of mosquitoes and housefly","volume":"38","author":"Hatakoshi","year":"1987","journal-title":"Med. Entomol. Zool."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"99","DOI":"10.7601\/mez.37.99","article-title":"Mosquito larvicidal activity of juvenile hormone active oxime ether compounds","volume":"37","author":"Hatakoshi","year":"1986","journal-title":"Med. Entomol. Zool."},{"key":"ref_39","first-page":"2889","article-title":"New insect juvenile hormone mimics: Thiolcarbamates","volume":"48","author":"Kisida","year":"1984","journal-title":"Agric. Biol. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1186\/1756-3305-7-299","article-title":"Insecticide resistance in disease vectors from Mayotte: An opportunity for integrated vector management","volume":"7","author":"Pocquet","year":"2014","journal-title":"Parasit. Vectors."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1093\/jee\/68.4.461","article-title":"Organophosphorus multiresistance in Culex pipiens quinquefasciatus in California","volume":"68","author":"Georghiou","year":"1975","journal-title":"J. Econ. Entomol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1093\/jme\/tjaa236","article-title":"NEVBD pesticide resistance monitoring network: Establishing a centralized network to increase regional capacity for pesticide resistance detection and monitoring","volume":"58","author":"Burtis","year":"2021","journal-title":"J. Med. Entomol."},{"key":"ref_43","first-page":"63","article-title":"Comparative action of 31 insecticides on chlorpyrifos-sensitive and resistant Culex pipiens (L.) larvae in the South of France","volume":"19","author":"Sinegre","year":"1977","journal-title":"Parassitologia"},{"key":"ref_44","first-page":"239","article-title":"Effects of sublethal dosages of insecticides on Culex quinquefasciatus","volume":"5","author":"Robert","year":"1989","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1590\/S0074-02761991000400027","article-title":"Tolerance of Culex quinquefasciatus Say, 1823 (Diptera:Culicidae) to methoprene in Cuba","volume":"86","author":"Ortega","year":"1991","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"305","DOI":"10.2987\/8756-971X(2005)21[305:IRICPF]2.0.CO;2","article-title":"Insecticide resistance in Culex pipiens from New York","volume":"21","author":"Paul","year":"2005","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kamita, S.G., Samra, A.I., Liu, J.Y., Cornel, A.J., and Hammock, B.D. (2011). Juvenile hormone (JH) esterase of the mosquito Culex quinquefasciatus is not a target of the JH analog insecticide methoprene. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028392"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"244","DOI":"10.2987\/18-6770.1","article-title":"Impact of storage and handling temperatures on the activities of mosquito larvicides","volume":"34","author":"Su","year":"2018","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_49","first-page":"61","article-title":"Evaluation of methoprene (Altosid) and diflubenzuron (Dimilin) for control of mosquito breeding in Tezpur (Assam)","volume":"33","author":"Baruah","year":"1996","journal-title":"Indian J. Malariol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1093\/jme\/tjy193","article-title":"High Resistance to Bacillus sphaericus and susceptibility to other common pesticides in Culex pipiens (Diptera: Culicidae) from Salt Lake City, UT","volume":"56","author":"Su","year":"2019","journal-title":"J. Med. Entomol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1603\/033.046.0421","article-title":"Susceptibility of Culex pipiens (Diptera: Culicidae) field populations in Cyprus to conventional organic insecticides, Bacillus thuringiensis subsp","volume":"46","author":"Vasquez","year":"2009","journal-title":"israelensis, and methoprene. J. Med. Entomol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"268","DOI":"10.2987\/22-7091","article-title":"Evaluation on the activity and efficacy of Omniprene\u2122 WSP and XWSP against the Southern House Mosquito Culex quinquefasciatus in simulated catch basins","volume":"38","author":"Su","year":"2022","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Lopez, K., Harbison, J., Irwin, P., Erkapic, A., Holub, R., Blanco, C., Paskewitz, S., Clifton, M., and Bartholomay, L. (2024). Extreme resistance to S-methoprene in field-collected Culex pipiens (Diptera: Culicidae) across the Chicago, IL region. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-69066-3"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1093\/jme\/tjad110","article-title":"Comparative bioactivity of S-methoprene and novel S-methobutene against mosquitoes (Diptera: Culicidae)","volume":"60","author":"Su","year":"2023","journal-title":"J. Med. Entomol."},{"key":"ref_55","first-page":"153","article-title":"Acute and sublethal effects of (S)-methoprene on some Australian mosquitoes","volume":"13","author":"Ritchie","year":"1997","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"250","DOI":"10.2987\/22-7084","article-title":"Lethal and sublethal concentrations of formulated larvicides against susceptible Aedes aegypti","volume":"38","author":"Aldridge","year":"2022","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/jmedent\/43.1.55","article-title":"Evaluation of novel insecticides for control of dengue vector Aedes aegypti (Diptera: Culicidae)","volume":"43","author":"Paul","year":"2006","journal-title":"J. Med. Entomol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1093\/jme\/tjz019","article-title":"Susceptibility profile of Aedes aegypti L. (Diptera: Culicidae) from Montclair, California, to commonly used pesticides, with note on resistance to pyriproxyfen","volume":"56","author":"Su","year":"2019","journal-title":"J. Med. Entomol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"105","DOI":"10.7601\/mez.26.105","article-title":"The effect of a juvenile hormone mimic, methoprene, against mosquito larvae","volume":"26","author":"Buei","year":"1975","journal-title":"Med. Entomol. Zool."},{"key":"ref_60","first-page":"290","article-title":"Sublethal effects of larval methoprene exposure on adult mosquito longevity","volume":"8","author":"Sawby","year":"1992","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1093\/jmedent\/42.5.830","article-title":"Effectiveness of methoprene, an insect growth regulator, against temephos-resistant Aedes aegypti populations from different Brazilian localities, under laboratory conditions","volume":"42","author":"Braga","year":"2005","journal-title":"J. Med. Entomol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"162","DOI":"10.2987\/24-7186","article-title":"Comparative laboratory activity and semi-field efficacy of Omniprene\u00ae G and Altosid\u00ae pellets against Aedes aegypti","volume":"40","author":"Hart","year":"2024","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1590\/S0074-02762005000400016","article-title":"Evaluation of methoprene effect on Aedes aegypti (Diptera: Culicidae) development in laboratory conditions","volume":"100","author":"Braga","year":"2005","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"106695","DOI":"10.1016\/j.actatropica.2022.106695","article-title":"The impact of insect growth regulators on adult emergence inhibition and the fitness of Aedes aegypti field populations in Thailand","volume":"236","author":"Fansiri","year":"2022","journal-title":"Acta Trop."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"173","DOI":"10.2987\/23-7134","article-title":"Comparative activity and efficacy of Sumilarv 0.5G and Altosid Xr briquet against Culex quinquefasciatus and Aedes aegypti in simulated catch basins","volume":"39","author":"Su","year":"2023","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_66","first-page":"72","article-title":"Comparative toxicity of selected larvicides and insect growth regulators to a Florida laboratory population of Aedes albopictus","volume":"11","author":"Ali","year":"1995","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Marcombe, S., Farajollahi, A., Healy, S.P., Clark, G.G., and Fonseca, D.M. (2014). Insecticide resistance status of United States populations of Aedes albopictus and mechanisms involved. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0101992"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1093\/jee\/toy071","article-title":"Bioefficacy of insect growth regulators against Aedes albopictus (Diptera: Culicidea) from Sarawak, Malaysia: A statewide survey","volume":"111","author":"Lau","year":"2018","journal-title":"J. Econ. Entomol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/jme\/tjab146","article-title":"Statewide efficacy assessment of insect growth regulators against Aedes albopictus (Diptera: Culicidae) in Sabah, Malaysia: An alternative control strategy?","volume":"59","author":"Chen","year":"2022","journal-title":"J. Med. Entomol."},{"key":"ref_70","first-page":"115","article-title":"Laboratory and field evaluation of some chemical and biological larvicides against Culex spp. (Diptera: Culicidae) immature stages","volume":"13","author":"Bayoumi","year":"2011","journal-title":"Int. J. Agric. Biol."},{"key":"ref_71","first-page":"68","article-title":"Larvicidal activity of water-soluble granular formulation of the insect growth regulator, pyriproxyfen, against Culex mosquitoes","volume":"6","author":"Kawada","year":"1994","journal-title":"Jpn. J. Environ. Entomol. Zool."},{"key":"ref_72","first-page":"409","article-title":"Potential for resistance to pyriproxyfen: A promising new mosquito larvicide","volume":"7","author":"Schaefer","year":"1991","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_73","first-page":"314","article-title":"Evaluation of new insect growth regulators against mosquitoes with notes on nontarget organisms","volume":"2","author":"Mulla","year":"1986","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1093\/jee\/81.6.1648","article-title":"Efficacy, nontarget effects, and chemical persistence of S-31183, a promising mosquito (Diptera: Culicidae) control agent","volume":"81","author":"Schaefer","year":"1988","journal-title":"J. Econ. Entomol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1093\/jmedent\/44.5.822","article-title":"Insecticide resistance in potential vector mosquitoes for West Nile virus in Japan","volume":"44","author":"Kasai","year":"2007","journal-title":"J. Med. Entomol."},{"key":"ref_76","first-page":"321","article-title":"Larvicidal effect of the juvenile hormone mimic pyriproxyfen on Culex pipiens","volume":"18","author":"Refaie","year":"2002","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"100138","DOI":"10.1016\/j.onehlt.2020.100138","article-title":"The synergistic effect of octopamine receptor agonists on selected insect growth regulators on Culex quinquefasciatus Say (Diptera: Culicidae) mosquitoes","volume":"10","author":"Ahmed","year":"2020","journal-title":"One Health."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"235","DOI":"10.13005\/bbra\/1115","article-title":"The biological effects of Sumilarv and Flubex on dengue fever vector Aedes aegypti in Jeddah Governorate Saudi Arabia","volume":"10","author":"Shaheen","year":"2013","journal-title":"Biosci. Biotechnol. Res. Asia."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1007\/s42690-020-00367-6","article-title":"Be quick or be dead: High temperatures reduce Aedes aegypti (Diptera: Culicidae) larval development time and pyriproxyfen larvicide efficiency in laboratory conditions","volume":"41","author":"Moura","year":"2021","journal-title":"Int. J. Trop. Insect Sci."},{"key":"ref_80","first-page":"7","article-title":"Laboratory studies on the efficacy and sublethal effects of an insect growth regulator, pyriproxyfen (S-31183) against Aedes aegypti (Linnaeus)","volume":"6","author":"Loh","year":"1989","journal-title":"Trop. Biomed."},{"key":"ref_81","first-page":"186","article-title":"Effect of pyriproxyfen in Aedes aegypti populations with different levels of susceptibility to the organophosphate temephos","volume":"32","author":"Andrighetti","year":"2008","journal-title":"Dengue Bulletin."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.actatropica.2009.10.015","article-title":"Resistance to the organophosphate temephos: Mechanisms, evolution and reversion in an Aedes aegypti laboratory strain from Brazil","volume":"113","author":"Gomes","year":"2010","journal-title":"Acta Trop."},{"key":"ref_83","first-page":"339","article-title":"Efficacy of pyriproxyfen in reference strains of Aedes aegypti susceptible and resistant to temephos","volume":"65","author":"Leyva","year":"2013","journal-title":"Rev. Cubana Med. Trop."},{"key":"ref_84","first-page":"71","article-title":"Evaluation of temephos and pyriproxyfen against Aedes aegypti (Diptera: Culicidae) from Guayaquil, Ecuador","volume":"66","author":"Leyva","year":"2014","journal-title":"Rev. Cubana Med. Trop."},{"key":"ref_85","first-page":"57","article-title":"Evaluation of two new insect growth regulators against mosquitoes in the laboratory","volume":"2","author":"Estrada","year":"1986","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_86","first-page":"344","article-title":"Utilization of bloodfed females of Aedes aegypti as a vehicle for the transfer of the insect growth regulator pyriproxyfen to larval habitats","volume":"10","author":"Itoh","year":"1994","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_87","first-page":"224","article-title":"Effect of pyriproxyfen for the Aedes (S) aegypti control (Diptera: Culicidae) in strains with various degrees of temephos resistance","volume":"62","author":"Leyva","year":"2010","journal-title":"Rev. Cubana Med. Trop."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"118","DOI":"10.4269\/ajtmh.2011.10-0335","article-title":"Field efficacy of new larvicide products for control of multi-resistant Aedes aegypti populations in Martinique (French West Indies)","volume":"84","author":"Marcombe","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1590\/S0074-02762011000800015","article-title":"Comparison of the insecticide susceptibilities of laboratory strains of Aedes aegypti and Aedes albopictus","volume":"106","author":"Seccacini","year":"2011","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1186\/s13071-018-3187-8","article-title":"Alternative insecticides for larval control of the dengue vector Aedes aegypti in Lao PDR: Insecticide resistance and semi-field trial study","volume":"11","author":"Marcombe","year":"2018","journal-title":"Parasit. Vectors."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"12","DOI":"10.52707\/1081-1710-48.1.12","article-title":"Assessment of insecticide resistance of Aedes aegypti (Diptera: Culicidae) populations to insect growth regulator pyriproxyfen, in the northeast region of Brazil","volume":"48","author":"Campos","year":"2023","journal-title":"J. Vector. Ecol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1093\/jmedent\/43.6.1190","article-title":"Laboratory evaluation of pyriproxyfen and spinosad, alone and in combination, against Aedes aegypti larvae","volume":"43","author":"Darriet","year":"2006","journal-title":"J. Med. Entomol."},{"key":"ref_93","first-page":"100875","article-title":"One does not simply apply larvicides: Aedes aegypti from Araraquara (Brazil) has reduced susceptibility to pyriproxyfen","volume":"41","author":"Moura","year":"2023","journal-title":"Vet. Parasit. Reg. Stud. Rep."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"398","DOI":"10.2987\/5697.1","article-title":"Effectiveness of pyriproxyfen and diflubenzuron formulations as larvicides against Aedes aegypti","volume":"24","author":"Seccacini","year":"2008","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"e20190489","DOI":"10.1590\/0037-8682-0489-2019","article-title":"Susceptibility of Aedes aegypti populations to pyriproxyfen in the Federal District of Brazil","volume":"53","author":"Carvalho","year":"2020","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1186\/s13071-020-04406-6","article-title":"Assessment of the susceptibility status of Aedes aegypti (Diptera: Culicidae) populations to pyriproxyfen and malathion in a nation-wide monitoring of insecticide resistance performed in Brazil from 2017 to 2018","volume":"13","author":"Campos","year":"2020","journal-title":"Parasit. Vectors."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"127","DOI":"10.7601\/mez.68.127","article-title":"Control of mosquito larvae in catch basins using pyriproxyfen and the mechanism underlying residual efficacy","volume":"68","author":"Ohashi","year":"2017","journal-title":"Med. Entomol. Zool."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Su, X., Guo, Y., Deng, J., Xu, J., Zhou, G., Zhou, T., Li, Y., Zhong, D., Kong, L., and Wang, X. (2019). Fast emerging insecticide resistance in Aedes albopictus in Guangzhou, China: Alarm to the dengue epidemic. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007665"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1046\/j.1365-2915.2003.00433.x","article-title":"Horizontal transfer of the insect growth regulator pyriproxyfen to larval microcosms by gravid Aedes albopictus and Ochlerotatus triseriatus mosquitoes in the laboratory","volume":"17","author":"Apperson","year":"2003","journal-title":"Med. Vet. Entomol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1603\/ME13068","article-title":"Effects of larval habitat substrate on pyriproxyfen efficacy against Aedes albopictus (Diptera: Culicidae)","volume":"50","author":"Suman","year":"2013","journal-title":"J. Med. Entomol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1186\/s13071-024-06485-1","article-title":"Study on the cross-resistance of Aedes albopictus (Skuse) (Diptera: Culicidae) to deltamethrin and pyriproxyfen","volume":"17","author":"Lin","year":"2024","journal-title":"Parasit. Vectors."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1584\/jpestics.R08-02","article-title":"Environmental fate and properties of pyriproxyfen","volume":"33","author":"Sullivan","year":"2008","journal-title":"J. Pesticide Sci."},{"key":"ref_103","unstructured":"(2025, February 20). Methoprene: PubChem Compound Summary for CID 5366546. National Center for Biotechnology Information (NCBI), Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/Methoprene."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"108926","DOI":"10.1016\/j.envres.2019.108926","article-title":"Systematic review of comparative studies assessing the toxicity of pesticide active ingredients and their product formulations","volume":"181","author":"Nagy","year":"2020","journal-title":"Environ. Res."},{"key":"ref_105","unstructured":"(2025, February 20). Pyriproxyfen: PubChem Compound Summary for CID 91753. National Center for Biotechnology Information (NCBI), Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/Pyriproxyfen."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s00436-020-06998-5","article-title":"Posttreatment temperature influences toxicity of insect growth regulators in Musca domestica","volume":"120","author":"Khan","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"16052","DOI":"10.1007\/s11356-020-08345-8","article-title":"Fate and ecotoxicological effects of pyriproxyfen in aquatic ecosystems","volume":"27","author":"Devillers","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1089\/vbz.2006.0562","article-title":"Spread of the Tiger: Global risk of invasion by the mosquito Aedes albopictus","volume":"7","author":"Benedict","year":"2007","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"10143","DOI":"10.1002\/ece3.3514","article-title":"Population genomics of the Asian tiger mosquito, Aedes albopictus: Insights into the recent worldwide invasion","volume":"7","author":"Kotsakiozi","year":"2017","journal-title":"Ecol. Evol."},{"key":"ref_110","unstructured":"Finney, D.J. (1971). Probit Analysis, Cambridge University Press."},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Robertson, J.L., Savin, N.E., Russell, R.M., and Preisler, H.K. (2007). Bioassays with Arthropods, CRC Press.","DOI":"10.1201\/9781420004045"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1897\/05-320R.1","article-title":"Comparing median lethal concentration values using confidence interval overlap or ratio tests","volume":"25","author":"Wheeler","year":"2006","journal-title":"Environ. Toxic Chem."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/4\/87\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:04:15Z","timestamp":1760029455000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/4\/87"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,28]]},"references-count":112,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["tropicalmed10040087"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed10040087","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,28]]}}}