{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:41:07Z","timestamp":1788932467161,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Humanidades Ciencia y Tecnolog\u00eda","award":["CF-2023-G-1265"],"award-info":[{"award-number":["CF-2023-G-1265"]}]},{"name":"Consejo Nacional de Humanidades Ciencia y Tecnolog\u00eda","award":["1001641"],"award-info":[{"award-number":["1001641"]}]},{"name":"scholarship","award":["CF-2023-G-1265"],"award-info":[{"award-number":["CF-2023-G-1265"]}]},{"name":"scholarship","award":["1001641"],"award-info":[{"award-number":["1001641"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Chagas disease is a neglected disease caused by the parasite Trypanosoma cruzi, a public health problem in both endemic and non-endemic countries. In Mexico, the southern region is considered endemic, and cases are frequently reported; however, in the northwestern region, only a few cases are confirmed annually. This study describes, for the first time, the Discrete Typing Unit (DTU) of Trypanosoma cruzi in a volunteer blood donor rejected for being reactive in the northwestern region of Mexico. Seroreactivity was confirmed using \u201cin-house\u201d ELISAs which employed three different antigens: total extract from Trypanosoma cruzi isolated from a vector (Triatoma rubida) from Sonora (strain T1), strain H1 and CL-Brener. The molecular characterization of Trypanosoma cruzi was conducted by amplifying satellite DNA by qPCR and posterior sequencing of the mini-exon gene, using Next Generation Sequencing (NGS) to enhance the accuracy of genetic characterization. The results show that the reactive status of this blood donor was confirmed using our in-house ELISAs, and the presence of Trypanosoma cruzi by detecting TcI DTU confirmed the infection status.<\/jats:p>","DOI":"10.3390\/tropicalmed10040104","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T05:38:26Z","timestamp":1744349906000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of Trypanosoma cruzi in a Reactive Blood Bank Sample in Sonora, Mexico"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7587-8893","authenticated-orcid":false,"given":"Idalia","family":"Paredes-Sotelo","sequence":"first","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4396-338X","authenticated-orcid":false,"given":"M\u00f3nica","family":"Res\u00e9ndiz-Sandoval","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, Mexico"},{"name":"Laboratorio de Inmunolog\u00eda, Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo A. C., Hermosillo C.P. 83304, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5985-8736","authenticated-orcid":false,"given":"Adriana","family":"Garibay-Escobar","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar Alfonso","family":"Paredes-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas y Agropecuarias, Universidad de Sonora, Caborca C.P. 83600, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8586-3387","authenticated-orcid":false,"given":"Aracely","family":"Angulo-Molina","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3896-7447","authenticated-orcid":false,"given":"Angel","family":"Ramos-Ligonio","sequence":"additional","affiliation":[{"name":"LADISER, Inmunolog\u00eda y Biolog\u00eda Molecular, Facultad de Ciencias Qu\u00edmicas, Universidad Veracruzana, Orizaba C.P. 94340, Veracruz, Mexico"},{"name":"Asociaci\u00f3n Chagas con Ciencia y Conocimiento A. C., Orizaba C.P. 94390, Veracruz, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9376-0209","authenticated-orcid":false,"given":"Eric","family":"Dumonteil","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Infectious Disease, Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9009-4201","authenticated-orcid":false,"given":"Claudia","family":"Herrera","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Infectious Disease, Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4456-2107","authenticated-orcid":false,"given":"Olivia","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101040","DOI":"10.1016\/j.lanepe.2024.101040","article-title":"Prevalence of Chagas disease among Latin American immigrants in non-endemic countries: An updated systematic review and meta-analysis","volume":"46","author":"Nascimento","year":"2024","journal-title":"Lancet Reg. 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