{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T19:31:39Z","timestamp":1779219099836,"version":"3.51.4"},"reference-count":83,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012359","name":"DBT","doi-asserted-by":"publisher","award":["BT\/PR25395\/NER\/95\/1177\/2017"],"award-info":[{"award-number":["BT\/PR25395\/NER\/95\/1177\/2017"]}],"id":[{"id":"10.13039\/100012359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012359","name":"DBT","doi-asserted-by":"publisher","award":["DST\/ICDBRICS\/PilotCall2\/MRP-TB (G)"],"award-info":[{"award-number":["DST\/ICDBRICS\/PilotCall2\/MRP-TB (G)"]}],"id":[{"id":"10.13039\/100012359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012359","name":"DBT","doi-asserted-by":"publisher","award":["RC4-21-012"],"award-info":[{"award-number":["RC4-21-012"]}],"id":[{"id":"10.13039\/100012359","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DST-BRICS","award":["BT\/PR25395\/NER\/95\/1177\/2017"],"award-info":[{"award-number":["BT\/PR25395\/NER\/95\/1177\/2017"]}]},{"name":"DST-BRICS","award":["DST\/ICDBRICS\/PilotCall2\/MRP-TB (G)"],"award-info":[{"award-number":["DST\/ICDBRICS\/PilotCall2\/MRP-TB (G)"]}]},{"name":"DST-BRICS","award":["RC4-21-012"],"award-info":[{"award-number":["RC4-21-012"]}]},{"DOI":"10.13039\/100013633","name":"IoE","doi-asserted-by":"publisher","award":["BT\/PR25395\/NER\/95\/1177\/2017"],"award-info":[{"award-number":["BT\/PR25395\/NER\/95\/1177\/2017"]}],"id":[{"id":"10.13039\/100013633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013633","name":"IoE","doi-asserted-by":"publisher","award":["DST\/ICDBRICS\/PilotCall2\/MRP-TB (G)"],"award-info":[{"award-number":["DST\/ICDBRICS\/PilotCall2\/MRP-TB (G)"]}],"id":[{"id":"10.13039\/100013633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013633","name":"IoE","doi-asserted-by":"publisher","award":["RC4-21-012"],"award-info":[{"award-number":["RC4-21-012"]}],"id":[{"id":"10.13039\/100013633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>The long regimen of drug therapy, the emergence of drug-resistance (DR), and infections with non-tuberculous mycobacteria (NTMs) are alarming challenges in controlling tuberculosis (TB), a disease caused by Mycobacterium tuberculosis (M.tb), necessitating the pursuit of new, broad-spectrum anti-mycobacterials. With more than two-thirds of the clinically useful antibiotics originating from the bacterial phylum Actinomycetota, and their enormous diversity in India, we explored atypical environments for new bacterial strains with potential anti-M.tb activity. In this study, we the examined the secondary metabolites of soil and endophytic bacterial isolates from the wetland niches of Manipur, India, and determined their anti-mycobacterial properties using viability assays. The ethyl acetate culture filtrate extracts of one of the isolates, named Streptomyces sp. SbAr007, showed broad-spectrum anti-mycobacterial activity against laboratory M.tb strains H37Ra and H37Rv, a clinical drug-resistant M.tb and non-tuberculous mycobacteria (NTM). The isolate was characterized for its phenotype and genetic identity, which indicated its closeness to Streptomyces samsunensis, Streptomyces malaysiensis, and Streptomyces solisilvae. Further, macrophage infection assays showed that the extracts could effectively control the intracellular mycobacterial growth but had negligible cytotoxicity to PBMCs from healthy donors. LC-MS identified an unusual combination of antibiotics in these culture filtrate extracts, which can be further explored for specific active molecules or as a formulation against DR-TB.<\/jats:p>","DOI":"10.3390\/tropicalmed10050117","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T06:38:37Z","timestamp":1745390317000},"page":"117","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Secondary Metabolites from a New Antibiotic-Producing Endophytic Streptomyces Isolate Inhibited Pathogenic and Multidrug-Resistant Mycobacterium tuberculosis Strains"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1378-8362","authenticated-orcid":false,"given":"Govinda Raju","family":"Vadankula","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Pathogenesis, Department of Biochemistry, School of Life Sciences, University of Hyderabad (UoH), Hyderabad 500046, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4043-7381","authenticated-orcid":false,"given":"Arshad","family":"Rizvi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pathogenesis, Department of Biochemistry, School of Life Sciences, University of Hyderabad (UoH), Hyderabad 500046, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1618-8469","authenticated-orcid":false,"given":"Haider","family":"Ali","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pathogenesis, Department of Biochemistry, School of Life Sciences, University of Hyderabad (UoH), Hyderabad 500046, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5952-6319","authenticated-orcid":false,"given":"Rakhi","family":"Khunjamayum","sequence":"additional","affiliation":[{"name":"Microbial Biotechnology Research Laboratory (MBRL), Department of Biochemistry, Manipur University, Canchipur 795003, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-3858","authenticated-orcid":false,"given":"V. V. Ramprasad","family":"Eedara","sequence":"additional","affiliation":[{"name":"MNR Medical College and Hospital, MNR Nagar, Fasalwadi, Narsapur Road, Sanga Reddy, Hyderabad 502294, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6420-9397","authenticated-orcid":false,"given":"Vijay","family":"Nema","sequence":"additional","affiliation":[{"name":"Molecular Biology Division, National Institute of Translational Virology and AIDS Research (Formerly National AIDS Research Institute), Pune 411026, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debananda Singh","family":"Ningthoujam","sequence":"additional","affiliation":[{"name":"Microbial Biotechnology Research Laboratory (MBRL), Department of Biochemistry, Manipur University, Canchipur 795003, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katragadda","family":"Suresh Babu","sequence":"additional","affiliation":[{"name":"Centre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prakasham Reddy","family":"Shetty","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry and Biotechnology Lab- Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shekhar C.","family":"Mande","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science, Pune 411007, India"},{"name":"Bioinformatics Centre, Savitribai Phule Pune University, Pune 411007, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharmistha","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pathogenesis, Department of Biochemistry, School of Life Sciences, University of Hyderabad (UoH), Hyderabad 500046, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,23]]},"reference":[{"key":"ref_1","unstructured":"CDC (2024, October 14). 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