{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:41:51Z","timestamp":1791618111907,"version":"4.3.4"},"reference-count":33,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Secretaria de Estado de Sa\u00fade de S\u00e3o Paulo (SES)","award":["479\/2020"],"award-info":[{"award-number":["479\/2020"]}]},{"name":"Fundo Especial de Sa\u00fade para Imuniza\u00e7\u00e3o em Massa e Controle de Doen\u00e7as (Fesima)","award":["479\/2020"],"award-info":[{"award-number":["479\/2020"]}]},{"name":"Fedial (Programa de Forma\u00e7\u00e3o para Investiga\u00e7\u00e3o Cient\u00edfica) scholarship from Instituto Adolfo Lutz","award":["479\/2020"],"award-info":[{"award-number":["479\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Yellow fever virus (YFV) is an endemic arbovirus in parts of Africa and the Americas. In Brazil, following the eradication of the urban transmission cycle, YFV is maintained in a sylvatic cycle involving several species of neotropical primates and mosquitoes of the genera Haemagogus and Sabethes, which serve as primary and secondary vectors, respectively. During the 2016\u20132019 outbreak in S\u00e3o Paulo State, a total of 3731 mosquito pools were collected from sites with ongoing epizootic events in 192 municipalities. The RT-qPCR analysis detected YFV in 46 pools (1.4%) across nine mosquito species, including both primary and secondary vectors, as well as species from the genera Aedes and Psorophora. Differences in viral loads were observed among species. While Aedes aegypti was not found to be positive, the detection of natural YFV infection in other Aedes species raises concerns about potential virus reurbanization. Further studies are needed to clarify the role of additional mosquito species in YFV transmission in Brazil.<\/jats:p>","DOI":"10.3390\/tropicalmed10050118","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:04:50Z","timestamp":1745467490000},"page":"118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Yellow Fever Virus (YFV) Detection in Different Species of Culicids Collected During an Outbreak in Southeastern Brazil, 2016\u20132019"],"prefix":"10.3390","volume":"10","author":[{"given":"Giovana Santos","family":"Caleiro","sequence":"first","affiliation":[{"name":"Center of Virology, Adolfo Lutz Institute, S\u00e3o Paulo 01246-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3803-559X","authenticated-orcid":false,"given":"Lucila Oliveira","family":"Vilela","sequence":"additional","affiliation":[{"name":"Cuny Graduate School of Public Health, New York, NY 10065, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0765-6451","authenticated-orcid":false,"given":"Karolina Morales Barrio","family":"Nuevo","sequence":"additional","affiliation":[{"name":"Center of Virology, Adolfo Lutz Institute, S\u00e3o Paulo 01246-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9688-5266","authenticated-orcid":false,"given":"Rosa Maria","family":"Tubaki","sequence":"additional","affiliation":[{"name":"Pasteur Institute, S\u00e3o Paulo 05508-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1778-437X","authenticated-orcid":false,"given":"Regiane Maria Tironi","family":"de Menezes","sequence":"additional","affiliation":[{"name":"Pasteur Institute, S\u00e3o Paulo 05508-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7766-7597","authenticated-orcid":false,"given":"Luis Filipe","family":"Mucci","sequence":"additional","affiliation":[{"name":"Pasteur Institute, Taubat\u00e9 12010-200, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1617-7340","authenticated-orcid":false,"given":"Juliana","family":"Telles-de-Deus","sequence":"additional","affiliation":[{"name":"Pasteur Institute, S\u00e3o Paulo 05508-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4928-0368","authenticated-orcid":false,"given":"Eduardo Sterlino","family":"Bergo","sequence":"additional","affiliation":[{"name":"Pasteur Institute, Ribeir\u00e3o Preto 14020-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7684-2883","authenticated-orcid":false,"given":"Emerson Luiz Lima","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Brazilian Ministry of Health, Bras\u00edlia 70719-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8903-604X","authenticated-orcid":false,"given":"Mariana Sequetin","family":"Cunha","sequence":"additional","affiliation":[{"name":"Center of Virology, Adolfo Lutz Institute, S\u00e3o Paulo 01246-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1089\/vbz.2016.2031","article-title":"Yellow Fever Remains a Potential Threat to Public Health","volume":"16","author":"Vasconcelos","year":"2016","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.3201\/eid2701.191220","article-title":"Differential Yellow Fever Susceptibility in New World Nonhuman Primates, Comparison with Humans, and Implications for Surveillance","volume":"27","author":"Guerra","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jcv.2014.08.030","article-title":"Yellow fever","volume":"64","author":"Monath","year":"2015","journal-title":"J. Clin. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1002\/jmv.2078","article-title":"Epidemic of jungle yellow fever in Brazil, 2000: Implications of climatic alterations in disease spread","volume":"65","author":"Vasconcelos","year":"2001","journal-title":"J. Med. Virol."},{"key":"ref_5","unstructured":"Ministerio da Sa\u00fade (2024, December 11). Bras\u00edlia-Df 2017 Guia de Vigil\u00e2ncia de Epizootias em Primatas n\u00e3o Humanos e Entomologia Aplicada \u00e0 Vigil\u00e2ncia da Febre Amarela Minist\u00e9rio da Sa\u00fade 2 a Edi\u00e7\u00e3o Atualizada, Available online: https:\/\/www.gov.br\/saude\/pt-br."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Li, S.L., Acosta, A.L., Hill, S.C., Brady, O.J., de Almeida, M.A.B., Cardoso, J.d.C., Hamlet, A., Mucci, L.F., de Deus, J.T., and Iani, F.C.M. (2022). Mapping environmental suitability of Haemagogus and Sabethes spp. mosquitoes to understand sylvatic transmission risk of yellow fever virus in Brazil. PLoS Neglected Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010019"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Silva-Inacio, C.L., and Ximenes, M.d.F.F.d.M. (Rev. Bras. Entomol., 2021). Haemagogus spegazzinii Br\u00e8thes, 1912 (Diptera: Culicidae) in Brazilian semiarid: Resistance in eggs and scale color variation in adults, Rev. Bras. Entomol., Epub ahead of print.","DOI":"10.1590\/1806-9665-rbent-2021-0078"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Cunha, M.S., da Costa, A.C., de Azevedo Fernandes, N.C.C., Santos, F.C.P.D., Nogueira, J.S., D\u2019Agostino, L.G., Komninakis, S.V., Witkin, S.S., Ressio, R.A., and Maeda, A.Y. (2019). Epizootics due to Yellow Fever Virus in S\u00e3o Paulo State, Brazil: Viral dissemination to new areas (2016\u20132017). Sci. Rep., 9.","DOI":"10.1038\/s41598-019-41950-3"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.3201\/eid2312.170685","article-title":"Outbreak of Yellow Fever among Nonhuman Primates, Espirito Santo, Brazil, 2017","volume":"23","author":"Fernandes","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Rezende, I.M., Sacchetto, L., Munhoz de Mello, \u00c9., Alves, P.A., Iani, F.C.M., Adelino, T.\u00c9.R., Duarte, M.M., Cury, A.L.F., Bernardes, A.F.L., and Santos, T.A. (2018). Persistence of Yellow fever virus outside the Amazon Basin, causing epidemics in Southeast Brazil, from 2016 to 2018. PLoS Neglected Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006538"},{"key":"ref_11","first-page":"e1","article-title":"Evidence for multiple sylvatic transmission cycles during the 2016\u20132017 yellow fever virus outbreak, Brazil","volume":"24","author":"Torres","year":"2018","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_12","unstructured":"Instituto Brasileiro de Geografia e Estat\u00edstica. Sao Paulo (2025, March 05). Available online: https:\/\/www.ibge.gov.br\/cidades-e-estados\/sp\/sao-paulo.html."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1126\/science.aat7115","article-title":"Genomic and epidemiological monitoring of yellow fever virus transmission potential","volume":"361","author":"Faria","year":"2018","journal-title":"Science"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Hill, S.C., de Souza, R., Th\u00e9z\u00e9, J., Claro, I., Aguiar, R.S., Abade, L., Santos, F.C.P., Cunha, M.S., Nogueira, J.S., and Salles, F.C.S. (2020). Genomic Surveillance of Yellow Fever Virus Epizootic in S\u00e3o Paulo, Brazil, 2016\u20132018. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008699"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Cunha, M.S., Tubaki, R.M., de Menezes, R.M.T., Pereira, M., Caleiro, G.S., Coelho, E., Saad, L.D.C., Fernandes, N.C.C.A., Guerra, J.M., and Nogueira, J.S. (2020). Possible non-sylvatic transmission of yellow fever between non-human primates in S\u00e3o Paulo city, Brazil, 2017\u20132018. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-72794-x"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2016). ggplot2: Elegant Graphics for Data Analysis, Springer.","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"de Oliveira, F.P., Stoffella-Dutra, A.G., Barbosa, C.G., Silva, d.O.J., Dourado, A.C., Duarte, S.J., Soares, R.K.L., Ara\u00fajo, J.J.P., Lacerda, N.M., and Zaz\u00e1, B.M.A. (2020). Re-Emergence of Yellow Fever in Brazil during 2016\u20132019: Challenges, Lessons Learned, and Perspectives. Viruses, 12.","DOI":"10.3390\/v12111233"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1080\/22221751.2019.1568180","article-title":"Haemagogus leucocelaenus and Haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in Brazil, 2016\u20132018","volume":"8","author":"Abreu","year":"2019","journal-title":"Emerg. Microbes Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1590\/S0036-46652011000300004","article-title":"Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in S\u00e3o Paulo State, Brazil, 2009","volume":"53","author":"Souza","year":"2011","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/S0035-9203(03)90023-X","article-title":"Isolations of yellow fever virus from Haemagogus leucocelaenus in Rio Grande do Sul State, Brazil","volume":"97","author":"Vasconcelos","year":"2003","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1948-7134.2014.12081.x","article-title":"Blood meal sources of mosquitoes captured in municipal parks in S\u00e3o Paulo, Brazil","volume":"39","author":"Malafronte","year":"2014","journal-title":"J. Vector Ecol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1590\/S0034-89101989000100003","article-title":"Prefer\u00eancias alimentares e domicilia\u00e7\u00e3o de mosquitos Culicidae no Vale do Ribeira, S\u00e3o Paulo, Brasil, com especial refer\u00eancia a Aedes scapularis e a Culex (Melanoconion)","volume":"23","author":"Forattini","year":"1989","journal-title":"Rev. Saude Publica"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1089\/vbz.2011.0730","article-title":"Isolation of Yellow Fever Virus from Mosquitoes in Misiones Province, Argentina","volume":"12","author":"Goenaga","year":"2012","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"de Oliveira, C.H., Andrade, M.S., Campos, F.S., Cardoso, J.d.C., Gon\u00e7alves-Dos-Santos, M.E., Oliveira, R.S., Aquino-Teixeira, S.M., Campos, A.A., AB Almeida, M., and Simonini-Teixeira, D. (2023). Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021. Viruses, 15.","DOI":"10.3390\/v15030757"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Stanzani, L.M.d.A., Motta, M.d.A., Erbisti, R.S., Abreu, F.V.S.d., Nascimento-Pereira, A.C., Ferreira-de-Brito, A., Neves, M.S.A.S., Pereira, G.R., Pereira, G.R., and Santos, C.B.d. (2022). Back to Where It Was First Described: Vectors of Sylvatic Yellow Fever Transmission in the 2017 Outbreak in Esp\u00edrito Santo, Brazil. Viruses, 14.","DOI":"10.3390\/v14122805"},{"key":"ref_26","first-page":"1","article-title":"Potential risk of re-emergence of urban transmission of Yellow Fever virus in Brazil facilitated by competent Aedes populations","volume":"7","author":"Madec","year":"2017","journal-title":"Environ. Sci. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"107110","DOI":"10.1016\/j.actatropica.2023.107110","article-title":"Phylogenetic analysis reveals a new introduction of Yellow Fever virus in S\u00e3o Paulo State, Brazil, 2023","volume":"251","author":"Fernandes","year":"2024","journal-title":"Acta Trop."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1590\/S0074-02762002000300031","article-title":"Oral Susceptibility to Yellow Fever Virus of Aedes aegypti from Brazil","volume":"97","author":"Vazeille","year":"2002","journal-title":"Mem\u00f3rias Inst. Oswaldo Cruz"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"25","DOI":"10.57148\/bepa.2019.v.16.37675","article-title":"Presen\u00e7a de Aedes aegypti e Aedes albopictus em ambientes urbanos adjacentes \u00e0s \u00e1reas silvestres que apresentam potencial para a circula\u00e7\u00e3o do v\u00edrus da febre amarela no estado de S\u00e3o Paulo","volume":"16","author":"Barbosa","year":"2022","journal-title":"BEPA Bol. Epidemiol\u00f3gico Paul."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Damasceno-Caldeira, R., Nunes-Neto, J.P., Arag\u00e3o, C.F., Freitas, M.N.O., Ferreira, M.S., de Castro, P.H.G., Dias, D.D., Ara\u00fajo, P.A.d.S., Brand\u00e3o, R.C.F., and Nunes, B.T.D. (2023). Vector Competence of Aedes albopictus for Yellow Fever Virus: Risk of Reemergence of Urban Yellow Fever in Brazil. Viruses, 15.","DOI":"10.20944\/preprints202303.0330.v1"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Amraoui, F., Pain, A., Piorkowski, G., Vazeille, M., Couto-Lima, D., de Lamballerie, X., Louren\u00e7o-De-Oliveira, R., and Failloux, A.-B. (2018). Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-32198-4"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Hamlet, A., Jean, K.K., Perea, W., Yactayo, S., Biey, J., Van Kerkhove, M., Ferguson, N., and Garske, T. (2018). The seasonal influence of climate and environment on yellow fever transmission across Africa. PLoS Neglected Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006284"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Sacchetto, L., Silva, N.I., De Rezende, I.M., Arruda, M.S., Costa, T.A., De Mello, \u00c9.M., Oliveira, G.F.G., Alves, P.A., De Mendon\u00e7a, V.E., and Stumpp, R.G.A.V. (2020). Neighbor danger: Yellow fever virus epizootics in urban and urban-rural transition areas of Minas Gerais state, during 2017\u20132018 yellow fever outbreaks in Brazil. PLoS Neglected Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008658"}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/5\/118\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:20:32Z","timestamp":1760030432000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/5\/118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":33,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["tropicalmed10050118"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed10050118","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,24]]}}}