{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T04:30:47Z","timestamp":1769574647720,"version":"3.49.0"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015997","name":"Brazilian Foundation for Biodiversity","doi-asserted-by":"publisher","award":["224\/2024"],"award-info":[{"award-number":["224\/2024"]}],"id":[{"id":"10.13039\/501100015997","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Via a One Health approach, this study concomitantly assessed the susceptibility of humans and dogs to Trypanosoma cruzi infections on three islands and in two mainland seashore areas of southern Brazil. Human serum samples were tested using an enzyme-linked immunosorbent assay (ELISA) to detect anti-T. cruzi antibodies, while dog serum samples were tested using indirect fluorescent antibodies in an immunofluorescence assay (IFA). Seropositive human and dog individuals were also tested using quantitative polymerase chain reaction (qPCR) in corresponding blood samples. Overall, 2\/304 (0.6%) human and 1\/292 dog samples tested seropositive for T. cruzi by ELISA and IFA, respectively, and these cases were also molecularly positive for T. cruzi by qPCR. Although a relatively low positivity rate was observed herein, these cases were likely autochthonous, and the individuals may have been infected as a consequence of isolated events of disturbance in the natural peridomicile areas nearby. Such a disturbance could come in the form of a fire or deforestation event, which can cause stress and parasitemia in wild reservoirs and, consequently, lead to positive triatomines. In conclusion, T. cruzi monitoring should always be conducted in suspicious areas to ensure a Chagas disease-free status over time. Further studies should also consider entomological and wildlife surveillance to fully capture the transmission and spread of T. cruzi on islands and in seashore mainland areas of Brazil and other endemic countries.<\/jats:p>","DOI":"10.3390\/tropicalmed10080220","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T10:48:08Z","timestamp":1754304488000},"page":"220","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["One Health Approach to Trypanosoma cruzi: Serological and Molecular Detection in Owners and Dogs Living on Oceanic Islands and Seashore Mainland of Southern Brazil"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0669-2952","authenticated-orcid":false,"given":"J\u00falia Iracema Moura","family":"Pacheco","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 (UFPR), Curitiba 80035-050, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6579-1278","authenticated-orcid":false,"given":"Louise Bach","family":"Kmetiuk","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Curitiba 81310-020, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Farias","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Curitiba 81310-020, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Curitiba 81310-020, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaronson Ramathan","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 (UFPR), Curitiba 80035-050, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7554-1520","authenticated-orcid":false,"given":"Leandro Meneguelli","family":"Biondo","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Studies, University of British Columbia, Kelowna, BC V1V 1V7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristielin Alves","family":"de Paula","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, S\u00e3o Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruana Renostro","family":"Delai","sequence":"additional","affiliation":[{"name":"Department of Animal Science, School of Life Sciences, Pontifical Catholic University of Paran\u00e1 (PUCPR), Curitiba 80215-901, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Turra","family":"Pimp\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Animal Science, School of Life Sciences, Pontifical Catholic University of Paran\u00e1 (PUCPR), Curitiba 80215-901, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8831-7376","authenticated-orcid":false,"given":"Jo\u00e3o Henrique","family":"Perotta","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 (UFPR), Curitiba 80035-050, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2380-4349","authenticated-orcid":false,"given":"Rog\u00e9rio","family":"Giuffrida","sequence":"additional","affiliation":[{"name":"Graduate College in Animal Sciences, University of Western S\u00e3o Paulo (UNOESTE), Presidente Prudente 19050-920, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7065-1138","authenticated-orcid":false,"given":"Vamilton Alvares","family":"Santar\u00e9m","sequence":"additional","affiliation":[{"name":"Graduate College in Animal Sciences, University of Western S\u00e3o Paulo (UNOESTE), Presidente Prudente 19050-920, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-0762","authenticated-orcid":false,"given":"Helio","family":"Langoni","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Studies, University of British Columbia, Kelowna, BC V1V 1V7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiano Borges","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Curitiba 81310-020, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4182-5821","authenticated-orcid":false,"given":"Alexander Welker","family":"Biondo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 (UFPR), Curitiba 80035-050, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan Roque de","family":"Barros Filho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 (UFPR), Curitiba 80035-050, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3597","DOI":"10.1111\/tbed.14723","article-title":"Epidemiology of Rickettsia spp. in Atlantic Rainforest Areas of Island and Seashore Mainland, Southern Brazil","volume":"69","author":"Kmetiuk","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Freitas, A.R., Delai, R.R., Kmetiuk, L.B., Gaspar, R.C., da Silva, E.C., Martini, R., Biondo, L.M., Giuffrida, R., de Barros Filho, I.R., and Santar\u00e9m, V.A. (2023). Spatial Owner-Dog Seroprevalence of Leptospira spp. Antibodies in Oceanic Islands and Costal Mainland of Southern Brazil. Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8040229"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Freitas, A.R., Delai, R.R., Kmetiuk, L.B., da Silva, E.C., Martini, R., Brand\u00e3o, A.P.D., Giuffrida, R., de Barros-Filho, I.R., Costa da Silva, R., and Langoni, H. (2022). Seropositivity of Anti-Toxoplasma Gondii Antibodies in Owners and Their Dogs Living on Island and Mainland Seashore Areas of Southern Brazil. Trop. Med. Infect. Dis., 7.","DOI":"10.3390\/tropicalmed7100252"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1089\/vbz.2023.0061","article-title":"Serological and Molecular Survey of Brucella Species in Owners and Their Dogs Living on Island and Mainland Seashore Areas of Brazil","volume":"24","author":"Barros","year":"2024","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kmetiuk, L.B., Gon\u00e7alves, G., Chechia Do Couto, A., Biondo, A.W., and Figueiredo, F.B. (2023). Serosurvey of Trypanosoma cruzi in Persons Experiencing Homelessness and Shelter Workers of Brazil. Front. Public. Health, 11.","DOI":"10.3389\/fpubh.2023.1125028"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e39","DOI":"10.1590\/s1678-9946202062039","article-title":"Awareness of Chagas Disease and Socioeconomic Characteristics of Bolivian Immigrants Living in Sao Paulo, Brazil","volume":"62","author":"Wanderley","year":"2020","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Soares, N.M., Barreto, N.M.P.V., Farias, M.M.B., de Lima Oliveira, C., Ara\u00fajo, W.A.C., de Souza, J.N., Teixeira, M.C.A., Gon\u00e7alves, N.L.S., Sampaio, D.D., and Pavan, T.B.S. (2023). Seroepidemiological Survey of Chronic Chagas Disease in a Rural Community in Southern Bahia, Brazil, Using Recombinant Chimeric Antigens. Pathogens, 12.","DOI":"10.3390\/pathogens12101222"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S0140-6736(17)31612-4","article-title":"Chagas Disease","volume":"391","author":"Molina","year":"2018","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1111\/tmi.13915","article-title":"Oral Transmission of Chagas Disease from a One Health Approach: A Systematic Review","volume":"28","author":"Gilabert","year":"2023","journal-title":"Trop. Med. Int. Health"},{"key":"ref_10","unstructured":"(2025, May 11). Global Report on Neglected Tropical Diseases 2023. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789240067295."},{"key":"ref_11","first-page":"705326","article-title":"Geographic Distribution of Chagas Disease Vectors in Brazil Based on Ecological Niche Modeling","volume":"2012","author":"Costa","year":"2012","journal-title":"J. Trop. Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ferro, E., Silva, A.M., Sobral-Souza, T., Vancine, M.H., Muylaert, R.L., de Abreu, A.P., Pelloso, S.M., de Barros Carvalho, M.D., de Andrade, L., and Ribeiro, M.C. (2018). Spatial Prediction of Risk Areas for Vector Transmission of Trypanosoma cruzi in the State of Paran\u00e1, Southern Brazil. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006907"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1186\/s13071-018-3067-2","article-title":"Trypanosoma cruzi Transmission in the Wild and Its Most Important Reservoir Hosts in Brazil","volume":"11","author":"Jansen","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e0712-2021","DOI":"10.1590\/0037-8682-0712-2021","article-title":"Profile of Natural Trypanosoma cruzi Infection among Dogs from Rural Areas of Southern Esp\u00edrito Santo, Brazil","volume":"55","author":"Pontes","year":"2022","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ferreira, S.S., Bueno, M.G., Nery, C.V.C., Rassi, F.B., Monticelli, C., Chagas, C.R.F., Gonzalez, I.H.L., Xavier, S.C.d.C., Lisboa, C.V., and Ramos, P.L. (2025). Trypanosoma cruzi (Chagas, 1909) Transmission among Captive Wild Mammals, Triatomines and Free-Living Opossums from Surrounding Areas in the S\u00e3o Paulo Zoological Park, Brazil. PLOS Neglected Trop. Dis., 19.","DOI":"10.1371\/journal.pntd.0013055"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"de Sousa Leite, L., de Rezende Feres, V.C., and Scalize, P.S. (2024). Predictor Variables in the Spread of Chagas Disease in Rural Areas. Pathogens, 13.","DOI":"10.3390\/pathogens13050394"},{"key":"ref_17","first-page":"101","article-title":"How to Enhance Atlantic Forest Protection? Dealing with the Shortcomings of Successional Stages Classification","volume":"22","author":"Resende","year":"2024","journal-title":"Perspect. Ecol. Conserv."},{"key":"ref_18","unstructured":"(2025, June 11). Parque Estadual Da Ilha Do Mel (PEIM), Available online: https:\/\/www.iat.pr.gov.br\/Pagina\/Parque-Estadual-da-Ilha-do-Mel-PEIM."},{"key":"ref_19","unstructured":"Coutinho-COORDENA\u00c7\u00c3O DE ELABORA\u00c7\u00c3O E REVIS\u00c3O DE PLANO DE MA.Pdf."},{"key":"ref_20","unstructured":"Stevenson, M., Sergeant, E., Heuer, C., Nunes, T., Heuer, C., Marshall, J., Sanchez, J., Thornton, R., Reiczigel, J., and Robison-Cox, J. (2025). epiR: Tools for the Analysis of Epidemiological Data, Repository CRAN, 2.0.85."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.actatropica.2013.10.002","article-title":"Prevalence of Chagas Disease in Brazil: A Systematic Review and Meta-Analysis","volume":"130","author":"Ramos","year":"2014","journal-title":"Acta Trop."},{"key":"ref_22","unstructured":"(2025, June 14). Chagatest_elisa_recombinante_v3_0_po.Pdf. Available online: https:\/\/access.wiener-lab.com\/VademecumDocumentos\/Vademecum%20portugues\/chagatest_elisa_recombinante_v4_0_po.pdf."},{"key":"ref_23","first-page":"227","article-title":"Fluorescent Antibody Test for the Serodiagnosis of American Trypanosomiasis. Technical Modification Employing Preserved Culture Forms of Trypanosoma cruzi in a Slide Test","volume":"8","author":"Camargo","year":"1966","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.vetpar.2009.06.027","article-title":"Leishmania spp. and\/or Trypanosoma cruzi Diagnosis in Dogs from Endemic and Nonendemic Areas for Canine Visceral Leishmaniasis","volume":"164","author":"Troncarelli","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_25","unstructured":"(2025, July 16). DNeasy Blood & Tissue Kits | DNA Purification | QIAGEN. Available online: https:\/\/www.qiagen.com\/us\/products\/discovery-and-translational-research\/dna-rna-purification\/dna-purification\/genomic-dna\/dneasy-blood-and-tissue-kit."},{"key":"ref_26","unstructured":"(2025, June 14). IU-IVD-008-05-Kit-IBMP-Biomol-Chagas.Pdf. Available online: https:\/\/www.ibmp.org.br\/biologia-molecular\/kit-biomol-chagas\/."},{"key":"ref_27","first-page":"100821","article-title":"Retrospect, Advances and Challenges in Chagas Disease Diagnosis: A Comprehensive Review","volume":"36","author":"Schijman","year":"2024","journal-title":"Lancet Reg. Health\u2013Am."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Coyle, P.V., Al Molawi, N.H., Kacem, M.A.B.H., El Kahlout, R.A., Al Kuwari, E., Al Khal, A., Gillani, I., Jeremijenko, A., Saeb, H., and Al Thani, M. (2022). Reporting of RT-PCR Cycle Threshold (Ct) Values during the First Wave of COVID-19 in Qatar Improved Result Interpretation in Clinical and Public Health Settings. J. Med. Microbiol., 71.","DOI":"10.1099\/jmm.0.001499"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/0035-9203(87)90423-8","article-title":"Evaluation of a Newly Developed Direct Agglutination Test (DAT) for Serodiagnosis and Sero-Epidemiological Studies of Visceral Leishmaniasis: Comparison with IFAT and ELISA","volume":"81","author":"Harith","year":"1987","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0035-9203(86)90033-7","article-title":"Serodiagnosis of Sudanese Visceral and Mucosal Leishmaniasis: Comparison of ELISA\u2014Immunofluorescence and Indirect Haemagglutination","volume":"80","author":"Wright","year":"1986","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"89","DOI":"10.4269\/ajtmh.24-0379","article-title":"Comparison of Four Chimeric Antigens and Commercial Serological Assays for the Diagnosis of Trypanosoma cruzi Infection","volume":"112","author":"Freitas","year":"2025","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_32","unstructured":"(2025, June 12). Acute Chagas Disease in Brazil from 2001 to 2018: A Nationwide Spatiotemporal Analysis | PLOS Neglected Tropical Diseases. Available online: https:\/\/journals.plos.org\/plosntds\/article?id=10.1371\/journal.pntd.0008445."},{"key":"ref_33","unstructured":"(2025, June 09). TabNet Win32 3.3: Doen\u00e7a De Chagas Aguda-Casos Confirmados Notificados No Sistema de Informa\u00e7\u00e3o de Agravos de Notifica\u00e7\u00e3o-Brasil, Available online: https:\/\/tabnet.datasus.gov.br\/cgi\/tabcgi.exe?sinannet\/cnv\/Chagasbr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1590\/0037-8682-0173-2017","article-title":"Epidemiological and Clinical Profile of Patients with Chagas Disease in the Central-North Area of Paran\u00e1, Southern Brazil","volume":"51","author":"Gasparim","year":"2018","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e20200225","DOI":"10.1590\/0037-8682-0225-2020","article-title":"Clinical and Epidemiological Aspects of Chronic Chagas Disease from Southern Brazil","volume":"53","author":"Lidani","year":"2020","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1089\/vbz.2018.2433","article-title":"Trypanosoma Found in Synanthropic Mammals from Urban Forests of Paran\u00e1, Southern Brazil","volume":"19","author":"Drozino","year":"2019","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0014-4894(02)00009-7","article-title":"Trypanosoma cruzi: Isoenzyme Analysis Suggests the Presence of an Active Chagas Sylvatic Cycle of Recent Origin in Paran\u00e1 State, Brazil","volume":"100","author":"Barnabe","year":"2002","journal-title":"Exp. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e31910212636","DOI":"10.33448\/rsd-v10i2.12636","article-title":"Transmiss\u00e3o oral da doen\u00e7a de Chagas: Uma revis\u00e3o de literatura","volume":"10","author":"Pacheco","year":"2021","journal-title":"Res. Soc. Dev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100946","DOI":"10.1016\/j.lanmic.2024.07.009","article-title":"Climate Change and Trypanosoma cruzi Transmission in North and Central America","volume":"5","author":"Forsyth","year":"2024","journal-title":"Lancet Microbe"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e200479","DOI":"10.1590\/0074-02760200479","article-title":"Chagas Disease in the Context of the 2030 Agenda: Global Warming and Vectors","volume":"117","author":"Souza","year":"2022","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1590\/S0074-02762002000400006","article-title":"Chagas Disease in Dogs from Endemic Areas of Costa Rica","volume":"97","author":"Montenegro","year":"2002","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_42","first-page":"100648","article-title":"Seroprevalence and Detection of Trypanosoma cruzi in Dogs Living in a Non-Endemic Area for Chagas Disease in the Legal Amazon Region, Brazil","volume":"26","author":"Costa","year":"2021","journal-title":"Vet. Parasitol. Reg. Stud. Reports"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.prevetmed.2015.04.014","article-title":"The Potential of Canine Sentinels for Reemerging Trypanosoma cruzi Transmission","volume":"120","author":"Naquira","year":"2015","journal-title":"Prev. Vet. Med."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Dur\u00e3es-Oliveira, J., Palma-Marques, J., Moreno, C., Rodrigues, A., Monteiro, M., Alexandre-Pires, G., da Fonseca, I.P., and Santos-Gomes, G. (2024). Chagas Disease: A Silent Threat for Dogs and Humans. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25073840"}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/8\/220\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:22:01Z","timestamp":1760034121000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/8\/220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,2]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["tropicalmed10080220"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed10080220","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,2]]}}}