{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:07:23Z","timestamp":1787162843434,"version":"build-2736575974"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Mexico has not only successfully eliminated dog-mediated human rabies in recent years, but also the last rabies case in a dog infected with the canine variant of the rabies virus was reported in 2016. Mass dog vaccination campaigns were the cornerstone of these achievements. Unfortunately, the rabies virus still circulates in wildlife and, thus, spill-over infections in humans, livestock, and pets, including dogs, still occurs. Especially dogs that cohabit at interfaces shared with wildlife, like shepherd dogs, are at risk. These dogs are often free-roaming and difficult to restrain for vaccination purposes. Oral rabies vaccination (ORV) as an alternative vaccination strategy was tested in several rural villages in Quer\u00e9taro State, Mexico. Bait acceptance and immunogenicity studies were conducted to test a licensed vaccine bait in terms of attractiveness and if the oral rabies vaccine strain, SPBN GASGAS, was able to induce an adequate immune response in local dogs, respectively. Although the egg(-flavored) bait was less well accepted (68.4%) by the dogs than the two other bait types included in the study, a bait made from boiled intestine segments (71.2%) and a bait with fish meal as an attractant (72.3%), dogs offered the egg bait were more often considered successfully vaccinated. 83.3% of the dogs offered an egg bait seroconverted during the immunogenicity study. Hence, ORV can be a suitable alternative by increasing the overall vaccination coverage of dogs that cannot be easily restrained and handled for vaccination.<\/jats:p>","DOI":"10.3390\/tropicalmed10090244","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T15:03:14Z","timestamp":1756393394000},"page":"244","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Feasibility of Oral Rabies Vaccination of Dogs in Mexico"],"prefix":"10.3390","volume":"10","author":[{"given":"Ver\u00f3nica","family":"Guti\u00e9rrez Cedillo","sequence":"first","affiliation":[{"name":"CENAPRECE, Ministry of Health, Marina Nacional N\u00b060, Piso 1 Col. Tacuba Miguel Hidalgo, Mexico City CP 11400, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Antonio","family":"Montoya Mondrag\u00f3n","sequence":"additional","affiliation":[{"name":"Ministry of Health of the Queretaro State, Privada Circunvalaci\u00f3n N\u00b06, Col. Jardines de Queretaro, Quer\u00e9taro CP 76020, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Ram\u00f3n","family":"Fern\u00e1ndez Col\u00edn","sequence":"additional","affiliation":[{"name":"CENAPRECE, Ministry of Health, Marina Nacional N\u00b060, Piso 1 Col. Tacuba Miguel Hidalgo, Mexico City CP 11400, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Bobe","sequence":"additional","affiliation":[{"name":"Ceva Innovation Center GmbH, Am Pharmapark, 06861 Dessau-Rosslau, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ad","family":"Vos","sequence":"additional","affiliation":[{"name":"Ceva Innovation Center GmbH, Am Pharmapark, 06861 Dessau-Rosslau, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Armando","family":"Lecuona Olivares","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Sierra Nevada 115, Lomas de Chapultepec, Miguel Hidalgo, Mexico City CP 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruy","family":"L\u00f3pez Ridaura","sequence":"additional","affiliation":[{"name":"CENAPRECE, Ministry of Health, Marina Nacional N\u00b060, Piso 1 Col. Tacuba Miguel Hidalgo, Mexico City CP 11400, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"ref_1","unstructured":"WHO (2024, June 28). Mexico Is Free from Human Rabies Transmitted by Dogs. Available online: https:\/\/www.who.int\/news\/item\/21-12-2019-mexico-is-free-from-human-rabies-transmitted-by-dogs."},{"key":"ref_2","unstructured":"Guti\u00e9rez, V. (2023, January 12\u201314). Mechanism and Benefits of Validation\/Verification of Elimination of Human Rabies Transmitted by Dogs and Canine Rabies. Proceedings of the 17th REDIPRA, Bogota, Columbia."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Puebla-Rodr\u00edguez, P., Almaz\u00e1n-Mar\u00edn, C., Garc\u00e9s-Ayala, F., Rend\u00f3n-Franco, E., Ch\u00e1vez-L\u00f3pez, S., G\u00f3mez-Sierra, M., Sandoval-Borja, A., Mart\u00ednez-Sol\u00eds, D., Escamilla-R\u00edos, B., and Sauri-Gonz\u00e1lez, I. (2023). Rabies virus in white-nosed coatis (Nasua narica) in Mexico: What do we know so far?. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1090222"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1089\/vbz.2021.0051","article-title":"The New Face of Human Rabies in Mexico, What\u2019s Next After Eradicating Rabies in Dogs","volume":"22","year":"2022","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_5","first-page":"167","article-title":"Rabies control in Mexico","volume":"131","author":"Baer","year":"2008","journal-title":"Dev. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Gonzalez-Roldan, J.F., Undurraga, E.A., Meltzer, M.I., Atkins, C., Vargas-Pino, F., Gutierrez Cedillo, V., and Ramon Hernandez-Perez, J. (2021). Cost-effectiveness of the national dog rabies prevention and control program in Mexico, 1990\u20132015. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009130"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Aboulfidaa, N., Cliquet, F., Robardet, E., Darkaoui, S., Wasniewski, M., Kaiser, C., Bobe, K., Vos, A., and Fassi Fihri, O. (2024). Evaluation of bait acceptance and immune response in local dogs during an oral rabies vaccination field study in Morocco. Trop. Med. Infect. Dis., 9.","DOI":"10.3390\/tropicalmed9070142"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bender, S., Bergman, D., Vos, A., Martin, A., and Chipman, R. (2017). Field studies evaluating bait acceptance and handling by dogs in Navajo Nation, USA. Trop. Med. Infect. Dis., 2.","DOI":"10.3390\/tropicalmed2020017"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"16","DOI":"10.20517\/ohir.2022.32","article-title":"Assessing Bait Acceptance of Local Dogs, Oral Vaccination Success and Human Contact Risk in Bali, Indonesia","volume":"3","author":"Husein","year":"2023","journal-title":"One Health Implement. Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Kasemsuwan, S., Chanachai, K., Pinyopummintr, T., Leelalapongsathon, K., Sujit, K., and Vos, A. (2018). Field studies evaluating bait acceptance and\u2014Handling by free-roaming dogs in Thailand. Vet. Sci., 5.","DOI":"10.3390\/vetsci5020047"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4743","DOI":"10.1016\/j.vaccine.2017.09.096","article-title":"Evaluation of Immune Responses in Dogs to Oral Rabies Vaccine under Field Conditions","volume":"37","author":"Smith","year":"2019","journal-title":"Vaccine"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Saputra, I.L.M., Suwarno, S., Husein, W.F., Suseno, P.P., Prayoga, I.M.A., Vos, A., Arthawan, I.M., Schoonman, L., Weaver, J., and Zainuddin, N. (2023). Immunogenicity of Oral Rabies Vaccine Strain SPBN GASGAS in Local Dogs in Bali, Indonesia. Viruses, 15.","DOI":"10.3390\/v15061405"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Molini, U., Hassel, R., Ortmann, S., Vos, A., Loschke, M., Shilongo, A., Freuling, C.M., and M\u00fcller, T. (2021). Immunogenicity of the oral rabies vaccine strain SPBN GASGAS in dogs under field settings in Namibia. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.737250"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5021","DOI":"10.1016\/j.vaccine.2020.05.047","article-title":"Oral bait preferences and feasibility of oral rabies vaccination in Bangladeshi dogs","volume":"38","author":"Bonwitt","year":"2020","journal-title":"Vaccine"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gibson, A.D., Mazeri, S., Yale, G., Desai, S., Naik, V., Corfmat, J., Ortmann, S., King, A., M\u00fcller, T., and Handel, I. (2019). Development of a non-meat based, mass producible and effective bait for oral vaccination of dogs against rabies in Goa State, India. Trop. Med. Infect. Dis., 4.","DOI":"10.3390\/tropicalmed4030118"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6162","DOI":"10.1016\/j.vaccine.2020.06.006","article-title":"Costs and effectiveness of alternative dog vaccination strategies to improve dog population coverage in rural and urban settings during a rabies outbreak","volume":"38","author":"Undurraga","year":"2020","journal-title":"Vaccine"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chanachai, K., Wongphruksasoong, V., Vos, A., Leelahapongsathon, K., Tangwangvivat, R., Sagarasaeranee, O., Lekcharoen, P., Trinuson, P., and Kasemsuwan, S. (2021). Feasibility and Effectiveness Studies with Oral Vaccination of Free-Roaming Dogs against Rabies in Thailand. Viruses, 13.","DOI":"10.3390\/v13040571"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Freuling, C.M., Busch, F., Vos, A., Ortmann, S., Lohr, F., Hedimbi, N., Josephat, P., Nelson, H.A., Shoombe, K., and Shilongo, A. (2022). Oral vaccination of dogs\u2014Experiences from a field trial in Namibia. PLoS Negl. Trop. Dis., 6.","DOI":"10.1101\/2022.04.21.488865"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100562","DOI":"10.1016\/j.onehlt.2023.100562","article-title":"Emergency response using oral rabies vaccination of dogs -field data from Namibia demonstrate high efficiency","volume":"16","author":"Freuling","year":"2023","journal-title":"One Health"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Leelahapongsathon, K., Kasemsuwan, S., Pinyopummintr, T., Boodde, O., Phawaphutayanchai, P., Aiyara, N., Bobe, K., Vos, A., Friedrichs, V., and M\u00fcller, T. (2020). Humoral immune response of Thai dogs after oral vaccination against rabies with the SPBN GASGAS vaccine strain. Vaccines, 8.","DOI":"10.3390\/vaccines8040573"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Langguth, A., Leelahapongsathon, K., Wannapong, N., Kasemsuwan, S., Ortmann, S., Vos, A., and B\u00f6er, M. (2021). Comparative Study of Optical Markers to assess Bait System Efficiency concerning Vaccine Release in the Oral Cavity of Dogs. Viruses, 13.","DOI":"10.3390\/v13071382"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Bobe, K., Ortmann, S., Kaiser, C., Perez-Bravo, D., Gethmann, J., Kliemt, J., K\u00f6rner, S., Theuss, T., Lindner, T., and Freuling, C. (2023). Efficacy of oral rabies vaccine baits containing SPBN GASGAS in domestic dogs according to international standards. Vaccines, 11.","DOI":"10.3390\/vaccines11020307"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/j.1863-2378.2008.01215.x","article-title":"Immunogenicity and safety of recombinant rabies viruses used for oral vaccination of stray dogs and wildlife","volume":"56","author":"Faber","year":"2009","journal-title":"Zoonoses Public Health"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"14141","DOI":"10.1128\/JVI.79.22.14141-14148.2005","article-title":"A single amino acid change in rabies virus glycoprotein increases virus spread and enhances virus pathogenicity","volume":"79","author":"Faber","year":"2005","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1128\/JVI.76.7.3374-3381.2002","article-title":"Overexpression of the rabies virus glycoprotein results in enhancement of apoptosis and antiviral immune response","volume":"76","author":"Faber","year":"2002","journal-title":"J. Virol."},{"key":"ref_26","unstructured":"Z\u00f6fel, P. (1988). Statistik in der Praxis, Fischer."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.antiviral.2017.03.023","article-title":"Successful strategies implemented towards the elimination of canine rabies in the Western Hemi-sphere","volume":"143","author":"Escobar","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_28","unstructured":"Vargas Pino, F., Guti\u00e9rrez Cedillo, V., and \u00cblvarez Lucas, C. (2009). Acceptance of Baits and Development of Immune Response Against the Rabies Virus in Canine Population of Mexico (Preliminary Report). Rabies in the Americas 2009, Available online: https:\/\/rabiesintheamericas.com\/wp-content\/uploads\/2009_RITA_XX.pdf."},{"key":"ref_29","first-page":"25","article-title":"Oral Vaccination of Dogs against Rabies","volume":"6","author":"Vos","year":"2019","journal-title":"Int. Anim. Health J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"310","DOI":"10.4269\/ajtmh.1992.47.310","article-title":"A field evaluation in Mexico of four baits for oral rabies vaccination of dogs","volume":"47","author":"Frontini","year":"1992","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_31","first-page":"145","article-title":"Bait Acceptability for Delivery of Oral Rabies Vaccine to Free-Ranging Dogs on the Navajo and Hopi Nations","volume":"131","author":"Bergman","year":"2008","journal-title":"Dev. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4750","DOI":"10.1016\/j.vaccine.2017.09.093","article-title":"Efficacy of the oral rabies virus vaccine strain SPBN GASGAS in foxes and raccoon dogs","volume":"37","author":"Freuling","year":"2019","journal-title":"Vaccine"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Moore, S., Gilbert, A., Vos, A., Freuling, C., Ellis, C., Kliemt, J., and M\u00fcller, T. (2017). Review of rabies virus antibodies from oral vaccination as a correlate of protection against lethal infection in animals. Trop. Med. Infect. Dis., 2.","DOI":"10.3390\/tropicalmed2030031"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Freuling, C.M., Te Kamp, V., Klein, A., G\u00fcnther, M., Zaeck, L., Potratz, M., Eggerbauer, E., Bobe, K., Kaiser, C., and Kretzschmar, A. (2019). Long-term immunogenicity and efficacy of the oral rabies virus vaccine strain SPBN GASGAS in foxes. Viruses, 11.","DOI":"10.3390\/v11090790"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"100410","DOI":"10.1016\/j.jvacx.2023.100410","article-title":"Long-term immunity in orally vaccinated dogs against rabies induced using SPBN GASGAS","volume":"15","author":"Vos","year":"2023","journal-title":"Vaccines X"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Te Kamp, V., Freuling, C.M., Vos, A., Schuster, P., Kaiser, C., Ortmann, S., Kretzschmar, A., Nemitz, S., Eggerbauer, E., and Ulrich, R. (2020). Oral rabies vaccination of wildlife: What drives vaccine uptake efficiency in different reservoir species?. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-59719-4"},{"key":"ref_37","first-page":"17","article-title":"Feasibility of oral rabies vaccination of dogs in Mexico: Bait acceptance and immunogenicity studies","volume":"27","author":"Colon","year":"2023","journal-title":"Rev. Asoc. Colomb. Infectologica"}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/9\/244\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:34:45Z","timestamp":1760034885000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/9\/244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,28]]},"references-count":37,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["tropicalmed10090244"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed10090244","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,28]]}}}