{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T13:03:48Z","timestamp":1769000628256,"version":"3.49.0"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"US DEPARTMENT OF DEFENSE, DEFENSE THREAT REDUCTION AGENCY","award":["HDTRA1-14-1-0029"],"award-info":[{"award-number":["HDTRA1-14-1-0029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Brucellosis is a widespread zoonotic disease and a major contributor to febrile illness, often underdiagnosed. This study investigated the seroprevalence of brucellosis and the associated exposure factors within South African farming and animal health communities. A cross-sectional survey was conducted across 40,000 km2 in the Free State and Northern Cape provinces from 2015 to 2016. Interviews and serum samples were collected from 847 volunteers on randomly selected farms and veterinary professionals listed in a regional register. Samples were tested using a commercial Brucella IgG ELISA. Risk factors were assessed using logistic regression, accounting for within-farm clustering. The seroprevalence was higher among veterinary professionals (11.6%; 16\/138) than farm-based participants (7.0%; 50\/711); p = 0.095. Multivariable analysis identified several exposure factors within the farm-based population: age over 40 years (aOR = 5.35; 95% CI: 1.68\u201317.02), White population group (aOR = 4.60; 95% CI: 1.64\u201312.91), contact with diseased animals (aOR = 2.01; 95% CI: 1.05\u20133.84), and working 4\u20138 h daily with ungulates (aOR = 2.90; 95% CI: 1.25\u20136.76). Among veterinary professionals, odds of exposure were higher with more than 5 years of work (OR = 1.82; 95% CI: 1.21\u20132.72) and extended ungulate contact (OR = 4.85; 95% CI: 1.27\u201318.52). Occupational exposure highlights the need for improved brucellosis prevention strategies.<\/jats:p>","DOI":"10.3390\/tropicalmed10110302","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T01:35:11Z","timestamp":1761269711000},"page":"302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Seroprevalence and Associated Risk Factors of Human Brucellosis in a Farming and Animal Health Community in South Africa, 2015\u20132016"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2840-5563","authenticated-orcid":false,"given":"Jennifer","family":"Rossouw","sequence":"first","affiliation":[{"name":"Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg 2192, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia N.","family":"Trataris-Rebisz","sequence":"additional","affiliation":[{"name":"Division for Biosafety and Biosecurity, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg 2192, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4395-347X","authenticated-orcid":false,"given":"Stefano","family":"Tempia","sequence":"additional","affiliation":[{"name":"Partnership for International Vaccine Initiative, Tkforce for Global Health, Atlanta, GA 30329, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6563-5280","authenticated-orcid":false,"given":"Melinda K.","family":"Rostal","sequence":"additional","affiliation":[{"name":"One Health Research Consulting, Glen Rock, NJ 07452, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6042-2860","authenticated-orcid":false,"given":"William B.","family":"Karesh","sequence":"additional","affiliation":[{"name":"One Health Concepts, Easton, CT 06612, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9929-4015","authenticated-orcid":false,"given":"Veerle","family":"Msimang","sequence":"additional","affiliation":[{"name":"Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg 2192, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s40249-020-00715-1","article-title":"Human brucellosis: Recent advances and future challenges","volume":"9","year":"2020","journal-title":"Infect. Dis. Poverty"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.3201\/eid2909.230052","article-title":"Global estimate of human brucellosis incidence","volume":"29","author":"Laine","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"249","DOI":"10.20506\/rst.32.1.2197","article-title":"Economics of brucellosis impact and control in low-income countries","volume":"32","author":"McDermott","year":"2013","journal-title":"Rev. Sci. Tech."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Moreno, E. (2014). Retrospective and prospective perspectives on zoonotic brucellosis. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00213"},{"key":"ref_5","unstructured":"Corbel, M.J. (2006). Brucellosis in Humans and Animals, World Health Organization."},{"key":"ref_6","first-page":"CD007179","article-title":"Antibiotics for treating human brucellosis","volume":"10","author":"Mehrani","year":"2012","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_7","first-page":"342","article-title":"Human brucellosis in South Africa","volume":"36","author":"Schrire","year":"1962","journal-title":"S. Afr. Med. J."},{"key":"ref_8","first-page":"2","article-title":"Brucellosis in the eastern Orange Free State","volume":"12","year":"1985","journal-title":"Epidemiol. Comments"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"883","DOI":"10.7196\/SAMJ.2016.v106i9.11020","article-title":"Human brucellosis in South Africa: Public health and diagnostic pitfalls","volume":"106","author":"Wojno","year":"2016","journal-title":"S. Afr. Med. J."},{"key":"ref_10","first-page":"110","article-title":"Brucellosis in South Africa\u2014a notifiable medical condition","volume":"16","author":"Frean","year":"2018","journal-title":"NICD Commun. Dis. Surveill. Bull."},{"key":"ref_11","first-page":"27","article-title":"Public health and economic importance of bovine brucellosis: An overview","volume":"5","author":"Pal","year":"2017","journal-title":"Am. J. Epidemiol. Infect. Dis."},{"key":"ref_12","unstructured":"Department of Agriculture, Forestry and Fisheries (DAFF) (2017). Discussion Paper on the Review of Bovine Brucellosis Control in South Africa."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"a1671","DOI":"10.4102\/ojvr.v86i1.1671","article-title":"Brucellosis knowledge, attitudes and practices of a South African communal cattle keeper group","volume":"86","author":"Cloete","year":"2019","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1089\/vbz.2017.2158","article-title":"Prevalence of selected zoonotic diseases and risk factors at a human-wildlife-livestock interface in Mpumalanga Province, South Africa","volume":"18","author":"Simpson","year":"2018","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Govindasamy, V., Etter, E.M.C., Harris, B.N., Rossouw, J., Abernethy, D.A., and Thompson, P.N. (2021). Knowledge of brucellosis, health-seeking behaviour, and risk factors for Brucella infection amongst workers on cattle farms in Gauteng, South Africa. Pathogens, 10.","DOI":"10.3390\/pathogens10111484"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Govindasamy, V., Thompson, P.N., Harris, B.N., Rossouw, J., Abernethy, D.A., and Etter, E.M.C. (2021). Bovine brucellosis in Gauteng, South Africa: Seroprevalence amongst cattle handlers and variables associated with seropositive cattle herds, 2014\u20132016. Pathogens, 10.","DOI":"10.3390\/pathogens10121547"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kolo, F.B., Adesiyun, A.A., Fasina, F.O., Harris, B.N., Rossouw, J., Byaruhanga, C., Geyer, H.D.W., Blumberg, L., Frean, J., and van Heerden, H. (2024). Brucellosis seropositivity using three serological tests and associated risk factors in abattoir workers in Gauteng Province, South Africa. Pathogens, 13.","DOI":"10.3390\/pathogens13010064"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Van der Westhuizen, C.G., Burt, F.J., Van Heerden, N., Van Zyl, W., Anthonissen, T., and Musoke, J. (2023). Prevalence and occupational exposure to zoonotic diseases in high-risk populations in the Free State Province, South Africa. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1196044"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Msimang, V., Thompson, P.N., Jansen van Vuren, P., Tempia, S., Cordel, C., Kgaladi, J., Khosa, J., Burt, F.J., Liang, J., and Rostal, M.K. (2019). Rift Valley fever virus exposure amongst farmers, farm workers, and veterinary professionals in central South Africa. Viruses, 11.","DOI":"10.3390\/v11020140"},{"key":"ref_20","first-page":"43","article-title":"Organizational research: Determining appropriate sample size in survey research","volume":"19","author":"Bartlett","year":"2001","journal-title":"Inf. Technol. Learn. Perform. J."},{"key":"ref_21","unstructured":"Vircell, S.L. (2006). Brucella ELISA IgG for In Vitro Diagnostic Use, Vircell. Parque Tecnologico de la Salud, Avicena 8, 18016 Granada, Spain."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Pereira, C.R., Cotrim de Almeida, J.V.F., Cardoso de Oliveira, I.R., Faria de Oliveira, L., Pereira, L.J., Zanger\u00f4nimo, M.G., Lage, A.P., and Dorneles, E.M.S. (2020). Occupational exposure to Brucella spp.: A systematic review and meta-analysis. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008164"},{"key":"ref_23","first-page":"813900","article-title":"Occupational exposure assessment and seroprevalence of Brucella Specific antibodies among veterinarians in the Northern Palestine","volume":"8","author":"Alzuheir","year":"2022","journal-title":"Front Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.actatropica.2015.10.023","article-title":"Brucellosis in Sub-Saharan Africa: Current challenges for management, diagnosis and control","volume":"165","author":"Ducrotoy","year":"2017","journal-title":"Acta Trop."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.actatropica.2017.12.026","article-title":"Brucellosis in West and Central Africa: A review of the current situation in a changing landscape of dairy cattle systems","volume":"179","author":"Craighead","year":"2018","journal-title":"Acta Trop."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1002\/vms3.425","article-title":"Brucellosis: Prevalence with reference to East African Community Countries\u2014A Rapid Review","volume":"7","author":"Djangwani","year":"2021","journal-title":"Vet. Med. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Tschopp, R., Gebregiorgis, A., Tassachew, Y., Andualem, H., Osman, M., Waqjira, M.W., Hattendorf, J., Mohammed, A., Hamid, M., and Molla, W. (2021). Integrated human-animal sero-surveillance of brucellosis in the pastoral Afar and Somali regions of Ethiopia. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009593"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Mwatondo, A., Muturi, M., Akoko, J., Nyamota, R., Nthiwa, D., Maina, J., Omolo, J., Gichuhi, S., Mureithi, M.W., and Bett, B. (2023). Seroprevalence and related risk factors of Brucella spp. in livestock and humans in Garbatula Subcounty, Isiolo County, Kenya. PLoS Negl. Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0011682"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Cadmus, S., Salam, S.P., Adesokan, H.K., Akporube, K., Ola-Daniel, F., and Awosanya, E.J. (2021). Seroprevalence of brucellosis and Q fever infections amongst pastoralists and their cattle herds in Sokoto State, Nigeria. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0254530"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"300","DOI":"10.4269\/ajtmh.23-0476","article-title":"Seroprevalence of brucellosis, knowledge, and risky practices in dairy cattle owners and workers in Maekel and Debub Regions, Eritrea","volume":"111","author":"Efrem","year":"2024","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Awah-Ndukum, J., Mouiche, M.M.M., Kouonmo-Ngnoyum, L., Bayang, H.N., Manchang, T.K., Poueme, R.S.N., Kouamo, J., Ngu-Ngwa, V., Assana, E., and Feussom, K.J.M. (2018). Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaound\u00e9r\u00e9, Cameroon. BMC Infect. Dis., 18.","DOI":"10.1186\/s12879-018-3522-x"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Mode, S., Ketterer, M., Qu\u00e9batte, M., and Dehio, C. (2022). Antibiotic persistence of intracellular Brucella abortus. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010635"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1093\/clinids\/14.1.131","article-title":"Specific antibody profile in human brucellosis","volume":"14","author":"Ariza","year":"1992","journal-title":"Clin. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1586\/eri.11.55","article-title":"Implications of laboratory diagnosis on brucellosis therapy","volume":"9","year":"2011","journal-title":"Expert Rev. Anti Infect. Ther."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/11\/302\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T04:26:53Z","timestamp":1761366413000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/10\/11\/302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,23]]},"references-count":34,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["tropicalmed10110302"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed10110302","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,23]]}}}