{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T16:12:19Z","timestamp":1763395939703,"version":"3.45.0"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian Defence Force Malaria and Infectious Disease Institute, Gallipoli Barracks, Enoggera, Queensland","award":["AFHSD-GEIS"],"award-info":[{"award-number":["AFHSD-GEIS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>This study represents the first investigation into the prevalence of pathogenic bacteria in isolated, free-ranging Eastern Grey Kangaroos (Macropus giganteus) inhabiting a human-shared environment. Samples were collected from the nasal and rectal passages of state-authorised culls of M. giganteus within a military training area, where recruits had reported recurrent cases of skin and soft tissue infections. The objective was to identify clinically relevant pathogenic microorganisms present in the nasal and rectal flora of these kangaroos. Analysis revealed carriage rates of 11% for methicillin-sensitive Staphylococcus aureus (MSSA) and 2% for methicillin-resistant S. aureus (MRSA). Other potentially pathogenic bacteria isolated included Pseudomonas spp., Streptococcus (Groups B and D), Acinetobacter spp., and multiple coagulase-negative Staphylococcus (CoNS) species. Notably, CoNS species were present in 17% of nasal isolates, with Mammaliicoccus sciuri (formerly Staphylococcus sciuri) detected in 41% of these isolates, suggesting a potential reservoir for antibiotic resistance genes and virulence factors. These findings support a One Health perspective, highlighting the interconnectedness of pathogenic bacteria, M. giganteus, humans, and their shared environment.<\/jats:p>","DOI":"10.3390\/tropicalmed10110322","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T15:38:09Z","timestamp":1763393889000},"page":"322","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human Pathogenic Bacteria Within the Nasal and Rectal Microbiome of Macropus giganteus"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5050-8206","authenticated-orcid":false,"given":"David","family":"Arroyo","sequence":"first","affiliation":[{"name":"Australian Defence Force Malaria and Infectious Disease Institute, Enoggera, QLD 4051, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Peart","sequence":"additional","affiliation":[{"name":"Microbiology Department, Melbourne Pathology, Collingwood, VIC 3066, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5708-5782","authenticated-orcid":false,"given":"Brian","family":"Vesely","sequence":"additional","affiliation":[{"name":"Australian Defence Force Malaria and Infectious Disease Institute, Enoggera, QLD 4051, Australia"},{"name":"Walter Reed Army Institute of Medical Science-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok 10400, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8365-1657","authenticated-orcid":false,"given":"Andrew","family":"Trudgian","sequence":"additional","affiliation":[{"name":"Australian Defence Force Malaria and Infectious Disease Institute, Enoggera, QLD 4051, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2602-5166","authenticated-orcid":false,"given":"Jessica","family":"Chellappah","sequence":"additional","affiliation":[{"name":"Australian Defence Force Malaria and Infectious Disease Institute, Enoggera, QLD 4051, Australia"},{"name":"School of Public Health, University of Queensland, Herston, QLD 4006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"ref_1","first-page":"1160","article-title":"Staphylococcus aureus: An overview of discovery, characteristics, epidemiology, virulence factors and antimicrobial sensitivity","volume":"8","author":"Rasheed","year":"2021","journal-title":"Eur. 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