{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T18:38:10Z","timestamp":1769279890704,"version":"3.49.0"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science & Technology Fundamental Resources Investigation Program","award":["2024FY100903"],"award-info":[{"award-number":["2024FY100903"]}]},{"DOI":"10.13039\/501100008111","name":"Key Research and Development Program in Hainan Province","doi-asserted-by":"publisher","award":["ZDYF2024SHFZ083"],"award-info":[{"award-number":["ZDYF2024SHFZ083"]}],"id":[{"id":"10.13039\/501100008111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008111","name":"Hainan Province Health Technology Innovation Joint Project","doi-asserted-by":"publisher","award":["WSJK2024MS226"],"award-info":[{"award-number":["WSJK2024MS226"]}],"id":[{"id":"10.13039\/501100008111","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guizhou Provincial Scientific and Technological Program","award":["183"],"award-info":[{"award-number":["183"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Angiostrongylus cantonensis (A. cantonensis) is the primary causative agent of human angiostrongyliasis and is widely distributed in Southeast Asia and China, with increasing reports from the Americas. Achatina fulica (A. fulica), Pomacea canaliculata (P. canaliculata), and slugs constitute established intermediate hosts of A. cantonensis, whereas Camaena hainanensis (C. hainanensis) has been newly reported as a host species in Hainan. A TaqMan quantitative PCR (qPCR) method assay targeting a novel genomic region of A. cantonensis was developed to detect infection in 150 snails collected from Hainan Province, China. The assay was employed to detect the parasite larvae across various snail tissues (lung sac, mucus, and foot), and its performance was compared with conventional lung sac microscopy. Out of the 120 A. fulica examined, 75 tested positive using the qPCR assay, yielding a significantly higher detection rate than lung-sac examination (p &lt; 0.05). Significant differences were also observed in the positivity rates across the three snail tissues (lung sac, mucus, and foot) (p &lt; 0.05), with the lung sac showing the highest rate of infection. Importantly, the detection of A. cantonensis DNA in snail mucus highlights its potential for development as a non-invasive diagnostic sample. Additionally, C. hainanensis was identified as a new host of A. cantonensis in Hainan, suggesting its possible contribution to parasite transmission. The newly developed qPCR assay demonstrated superior sensitivity (reflected by lower Ct values) compared with previously published TaqMan qPCR methods. The established qPCR method provides a sensitive and non-invasive tool for detecting A. cantonensis in snails, and can be applied for monitoring and early warning of parasite prevalence and transmission.<\/jats:p>","DOI":"10.3390\/tropicalmed11020034","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T14:58:40Z","timestamp":1769180320000},"page":"34","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a TaqMan qPCR Method for Detecting Angiostrongylus cantonensis (Rhabditida: Angiostrongylidae) Infection in Snails from Hainan Province, China"],"prefix":"10.3390","volume":"11","author":[{"given":"Kun","family":"Wang","sequence":"first","affiliation":[{"name":"School of Public Health, Hangzhou Medical College, Hangzhou 310013, China"},{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai 200025, China"},{"name":"National Health Commission of the People\u2019s Republic of China (NHC) Key Laboratory of Parasite and Vector Biology, Shanghai 200025, China"},{"name":"World Health Organization (WHO) Collaborating Center for Tropical Diseases, Shanghai 200025, China"},{"name":"National Center for International Research on Tropical Diseases, Shanghai 200025, China"},{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2168-5999","authenticated-orcid":false,"given":"Tian","family":"Tian","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai 200025, China"},{"name":"National Health Commission of the People\u2019s Republic of China (NHC) Key Laboratory of Parasite and Vector Biology, Shanghai 200025, China"},{"name":"World Health Organization (WHO) Collaborating Center for Tropical Diseases, Shanghai 200025, China"},{"name":"National Center for International Research on Tropical Diseases, Shanghai 200025, China"},{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunhai","family":"Guo","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai 200025, China"},{"name":"National Health Commission of the People\u2019s Republic of China (NHC) Key Laboratory of Parasite and Vector Biology, Shanghai 200025, China"},{"name":"World Health Organization (WHO) Collaborating Center for Tropical Diseases, Shanghai 200025, China"},{"name":"National Center for International Research on Tropical Diseases, Shanghai 200025, China"},{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muxin","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai 200025, China"},{"name":"National Health Commission of the People\u2019s Republic of China (NHC) Key Laboratory of Parasite and Vector Biology, Shanghai 200025, China"},{"name":"World Health Organization (WHO) Collaborating Center for Tropical Diseases, Shanghai 200025, China"},{"name":"National Center for International Research on Tropical Diseases, Shanghai 200025, China"},{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaonen","family":"Wu","sequence":"additional","affiliation":[{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiying","family":"Hou","sequence":"additional","affiliation":[{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binbin","family":"Xie","sequence":"additional","affiliation":[{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanna","family":"Wei","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese 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Shanghai 200025, China"},{"name":"World Health Organization (WHO) Collaborating Center for Tropical Diseases, Shanghai 200025, China"},{"name":"National Center for International Research on Tropical Diseases, Shanghai 200025, China"},{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"},{"name":"School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou 310013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhisheng","family":"Dang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai 200025, China"},{"name":"National Health Commission of the People\u2019s Republic of China (NHC) Key Laboratory of Parasite and Vector Biology, Shanghai 200025, China"},{"name":"World Health Organization (WHO) Collaborating Center for Tropical Diseases, Shanghai 200025, China"},{"name":"National Center for International Research on Tropical Diseases, Shanghai 200025, China"},{"name":"Hainan Tropical Diseases Research Center, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dingwei","family":"Sun","sequence":"additional","affiliation":[{"name":"Hainan Provincial Center for Disease Control and Prevention, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Hong","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai 200025, China"},{"name":"National Health Commission of the People\u2019s Republic of China (NHC) Key Laboratory of Parasite and Vector Biology, 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