{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T17:35:43Z","timestamp":1772559343248,"version":"3.50.1"},"reference-count":33,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2014CNPq","doi-asserted-by":"publisher","award":["432056\/2016-5"],"award-info":[{"award-number":["432056\/2016-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005286","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Mato Grosso\u2014FAPEMAT","doi-asserted-by":"publisher","award":["0226317\/2017"],"award-info":[{"award-number":["0226317\/2017"]}],"id":[{"id":"10.13039\/501100005286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>In countries where Dengue virus is endemic, the occurrence of outbreaks and epidemic events is strongly associated with viral genomic evolution. In addition, the introduction of a new agent, such as Zika virus, in a naive population and its concomitant circulation may increase mutations and virulence. This study aimed to characterize the molecular patterns and circulation of Zika and Dengue viruses inland of midwestern Brazil. Samples from reported cases of zika and dengue fever were subjected to molecular and phylogenetic analyses. Partial genomes of these viruses were recovered and characterized from six samples. Phylogenetic analysis revealed that the Zika virus clustered within the American strain of Asian\/American lineage and Dengue virus grouped within the Brazilian lineage (BR04) of serotype 2 from the Asian\/American genotype. Amino acid substitutions, and consequently nonsynonymous mutations, were identified in the RdRp domain of the NS5 protein coding region in the recovered genomes from both viruses. These findings highlight the importance of molecular epidemiological surveillance, especially in endemic regions with cocirculation and substantial epidemic risk. Ongoing monitoring efforts are crucial to better understand viral evolution and its potential impact on future outbreaks and epidemic dynamics.<\/jats:p>","DOI":"10.3390\/tropicalmed11030068","type":"journal-article","created":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:22:21Z","timestamp":1772551341000},"page":"68","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mutations in the NS5 RdRp Domain of Zika and Dengue Viruses: Insights into Molecular Patterns in Inland Midwestern Brazil"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7990-6750","authenticated-orcid":false,"given":"Jos\u00e9 Henrique Francisco","family":"Roma","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Cl\u00ednica M\u00e9dica, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas, Campinas 13083-894, Brazil"},{"name":"Faculdade de Ci\u00eancias da Sa\u00fade, Universidade Federal de Rondon\u00f3polis, Rondonopolis 78736-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel Cruz","family":"Alves","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias da Sa\u00fade, Universidade Federal de Rondon\u00f3polis, Rondonopolis 78736-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2355-7907","authenticated-orcid":false,"given":"Bruno Moreira","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias da Sa\u00fade, Universidade Federal de Rondon\u00f3polis, Rondonopolis 78736-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata Dezengrini","family":"Slhessarenko","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiaba 78060-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2568-9601","authenticated-orcid":false,"given":"Juliana Helena","family":"Chavez-Pavoni","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias da Sa\u00fade, Universidade Federal de Rondon\u00f3polis, Rondonopolis 78736-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u00e2ngela Ribeiro","family":"Resende","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Cl\u00ednica M\u00e9dica, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas, Campinas 13083-894, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Harapan, H., Michie, A., Sasmono, R.T., and Imrie, A. 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