{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:13:28Z","timestamp":1785420808860,"version":"3.56.0"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N42A670561"],"award-info":[{"award-number":["N42A670561"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"award":["N42A670561"],"award-info":[{"award-number":["N42A670561"]}],"id":[{"id":"https:\/\/ror.org\/018wfhg78","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004071","name":"Khon Kaen University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004071","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/03cq4gr50","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Background: To address the significant burden of helminthiases in Thailand, this cross-sectional study compared the performance of a fecal parasite concentrator kit (FPCK) against the Kato\u2013Katz (KK) method for diagnosing intestinal parasites in endemic populations across the Northeast and Southern regions. Methods: Stool samples were collected from 140 participants and examined for intestinal parasitic infections using both FPCK and KK methods. Results: The FPCK method demonstrated a significantly higher detection rate of 45.0% compared to 35.0% for the KK method. For detecting liver fluke (Opisthorchis viverrini), the FPCK method detected significantly more cases than the KK method (10.71% vs. 4.29%) (p = 0.0027). For other parasites such as Trichuris trichiura, Strongyloides stercoralis, and Entamoeba coli, the FPCK method tended to detect more infections, but the differences were not statistically significant (p &gt; 0.05). Conclusions: The FPCK method showed better performance than the KK method for detecting intestinal helminth infections in stool samples, particularly O. viverrini, T. trichiura, S. stercoralis, and Entamoeba coli. Therefore, FPCK could be used as a suitable stool examination method for surveillance and monitoring of preventive treatment for opisthorchiasis.<\/jats:p>","DOI":"10.3390\/tropicalmed11050118","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T12:31:56Z","timestamp":1777465916000},"page":"118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Parasite Concentrator Kit and Kato\u2013Katz Method for Detection of Intestinal Parasites in Stool Samples"],"prefix":"10.3390","volume":"11","author":[{"given":"Penchom","family":"Janwan","sequence":"first","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Hematology and Transfusion Science Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0462-6209","authenticated-orcid":false,"given":"Lakkhana","family":"Sadaow","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5873-2392","authenticated-orcid":false,"given":"Patcharaporn","family":"Boonroumkaew","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutchanee","family":"Rodpai","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0451-0756","authenticated-orcid":false,"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2604-0281","authenticated-orcid":false,"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pokkamol","family":"Laoraksawong","sequence":"additional","affiliation":[{"name":"Department of Occupational Safety and Environmental Health, Faculty of Public Health, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2138-8783","authenticated-orcid":false,"given":"Pewpan Maleewong","family":"Intapan","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6726-3759","authenticated-orcid":false,"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1172\/JCI34261","article-title":"Helminth infections: The great neglected tropical diseases","volume":"118","author":"Hotez","year":"2008","journal-title":"J. Clin. Investig."},{"key":"ref_2","unstructured":"World Health Organization (2025, May 26). Global Report on Neglected Tropical Disease. Available online: https:\/\/iris.who.int\/bitstream\/handle\/10665\/365729\/9789240067295-eng.pdf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"106133","DOI":"10.1016\/j.actatropica.2021.106133","article-title":"Current status of human liver fluke infections in the Greater Mekong Subregion","volume":"224","author":"Sripa","year":"2021","journal-title":"Acta Trop."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e59755","DOI":"10.7554\/eLife.59755","article-title":"Model-based spatial-temporal mapping of opisthorchiasis in endemic countries of Southeast Asia","volume":"10","author":"Zhao","year":"2021","journal-title":"eLife"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.actatropica.2003.01.002","article-title":"Opisthorchiasis control in Thailand","volume":"88","author":"Jongsuksuntigul","year":"2003","journal-title":"Acta Trop."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e0000923","DOI":"10.1128\/cmr.00009-23","article-title":"Clonorchiasis and opisthorchiasis: Epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control","volume":"37","author":"Qian","year":"2024","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0065-308X(10)72011-X","article-title":"Food-borne trematodiases in Southeast Asia epidemiology, pathology, clinical manifestation and control","volume":"72","author":"Sripa","year":"2010","journal-title":"Adv. Parasitol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1038\/s41572-021-00300-2","article-title":"Cholangiocarcinoma","volume":"7","author":"Brindley","year":"2021","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6825","DOI":"10.7314\/APJCP.2015.16.16.6825","article-title":"Review and current status of Opisthorchis viverrini infection at the community level in Thailand","volume":"16","author":"Kaewpitoon","year":"2015","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1756-3305-7-37","article-title":"Global numbers of infection and disease burden of soil transmitted helminth infections in 2010","volume":"7","author":"Pullan","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1016\/j.jiph.2024.03.005","article-title":"The global prevalence of Trichuris trichiura infection in humans (2010\u20132023): A systematic review and meta-analysis","volume":"17","author":"Behniafar","year":"2024","journal-title":"J. Infect. Public Health"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00436-021-07365-8","article-title":"The prevalence of human trichuriasis in Asia: A systematic review and meta-analysis","volume":"121","author":"Badri","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"28432","DOI":"10.1038\/s41598-025-14221-7","article-title":"Prevalence, associated risk factors and satellite imagery analysis in predicting soil-transmitted helminth infection in Nakhon Si Thammarat Province, Thailand","volume":"15","author":"Muenjak","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1016\/j.amjms.2017.09.003","article-title":"Trichuriasis","volume":"355","author":"Bansal","year":"2018","journal-title":"Am. J. Med. Sci."},{"key":"ref_15","unstructured":"World Health Organization (2023, November 25). Strongyloidiasis. Available online: https:\/\/www.who.int\/teams\/control-of-neglected-tropical-diseases\/soil-transmitted-helminthiases\/strongyloidiasis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e0003323","DOI":"10.1128\/cmr.00033-23","article-title":"Human strongyloidiasis: Complexities and pathways forward","volume":"36","author":"Buonfrate","year":"2023","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1186\/s13071-022-05284-w","article-title":"Mapping the global distribution of Strongyloides stercoralis and hookworms by ecological niche modeling","volume":"15","author":"Fleitas","year":"2022","journal-title":"Parasites Vectors"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Prasongdee, T.K., Laoraksawong, P., Kanarkard, W., Kraiklang, R., Sathapornworachai, K., Naonongwai, S., Laummaunwai, P., Sanpool, O., Intapan, P.M., and Maleewong, W. (2017). An eleven-year retrospective hospital-based study of epidemiological data regarding human strongyloidiasis in northeast Thailand. BMC Infect. Dis., 17.","DOI":"10.1186\/s12879-017-2723-z"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.idc.2018.10.006","article-title":"Strongyloidiasis: A neglected tropical disease","volume":"33","author":"Krolewiecki","year":"2019","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_20","first-page":"20222204","article-title":"Diagnosis of helminths depends on worm fecundity and the distribution of parasites within hosts","volume":"290","author":"Crellen","year":"2023","journal-title":"Proc. Biol. Sci."},{"key":"ref_21","unstructured":"World Health Organization (2025, October 10). Prevention and Control of Schistosomiasis and Soil-Transmitted Helminthiasis: Report of a WHO Expert Committee. Available online: https:\/\/iris.who.int\/server\/api\/core\/bitstreams\/0fb84288-9083-49f4-967d-c6cfb98210a3\/content."},{"key":"ref_22","unstructured":"(2025, October 10). Centers for Disease Control and Prevention (CDC)\u2014DPDx\u2014Diagnostic Procedures\u2014Stool Specimens-Specimens Processing, Available online: https:\/\/www.cdc.gov\/dpdx\/diagnosticprocedures\/stool\/index.html."},{"key":"ref_23","first-page":"397","article-title":"A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni","volume":"14","author":"Katz","year":"1972","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jbi.2014.02.013","article-title":"Sample size estimation in diagnostic test studies of biomedical informatics","volume":"48","year":"2014","journal-title":"J. Biomed. Inform."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1186\/s13071-022-05338-z","article-title":"Performance of Mini Parasep\u00ae SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand","volume":"15","author":"Kopolrat","year":"2022","journal-title":"Parasites Vectors"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1093\/trstmh\/try010","article-title":"Diagnostic performance of Mini Parasep\u00ae solvent-free faecal parasite concentrator relative to Kato-Katz and McMaster for the diagnosis of intestinal parasitic infections","volume":"111","author":"Adugna","year":"2017","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"29","DOI":"10.4103\/tp.TP_44_17","article-title":"A comparative study of formalin-ethyl acetate sedimentation technique and Mini Parasep\u00ae solvent-free method in the rapid diagnosis of intestinal parasites","volume":"8","author":"Khanna","year":"2018","journal-title":"Trop. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.actatropica.2018.12.018","article-title":"Comparison of stool examination techniques to detect Opisthorchis viverrini in low intensity infection","volume":"191","author":"Charoensuk","year":"2019","journal-title":"Acta Trop."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/11\/5\/118\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:11:19Z","timestamp":1777608679000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/11\/5\/118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":28,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["tropicalmed11050118"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed11050118","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]}}}