{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T05:02:39Z","timestamp":1780117359021,"version":"3.54.0"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2026JB16"],"award-info":[{"award-number":["2026JB16"]}]},{"name":"Shanghai Sailing Program","award":["24YF2756800"],"award-info":[{"award-number":["24YF2756800"]}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"crossref","award":["CAAS-ASTIP-2025-SHVRI"],"award-info":[{"award-number":["CAAS-ASTIP-2025-SHVRI"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Although schistosome eggs are widely recognized as the principal drivers of hepatic granulomatous inflammation and fibrosis, the independent effects of adult worms may be masked by strong egg antigen-mediated responses. This study aimed to investigate whether adult Schistosoma japonicum worms alter the miRNA expression profile of hepatic stellate cells and to explore the potential relevance of these changes to liver fibrosis and hepatocellular carcinoma-related processes. A non-contact Transwell co-culture system was established using paired Schistosoma japonicum worms or male worms and hepatic stellate cells. Male worms were additionally included to further assess worm-derived effects independent of egg production\u2013related influences. Untreated hepatic stellate cells served as controls. Total RNA was extracted for miRNA sequencing, and differentially expressed miRNAs were identified. Target gene prediction, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, and validation using The Cancer Genome Atlas database were subsequently performed. Both paired worms and male worms significantly altered the miRNA expression profile of hepatic stellate cells. Several differentially expressed miRNAs were identified, among which hsa-miR-103a-3p showed relatively stable changes. Pathway enrichment analysis suggested that the potential target genes of hsa-miR-103a-3p were mainly enriched in AMP-activated protein kinase, mechanistic target of rapamycin, tumor necrosis factor, insulin signaling, and cellular senescence pathways. Further analysis using The Cancer Genome Atlas database showed that hsa-miR-103a-3p had diagnostic value in hepatocellular carcinoma and was associated with alpha-fetoprotein level, albumin level, Ishak fibrosis score, pathological stage, histological type, and tumor status. These findings suggest that adult S. japonicum worms may alter the miRNA expression profile of hepatic stellate cells, and that hsa-miR-103a-3p may be associated with fibrogenic responses and may have potential relevance to hepatocellular carcinoma-related processes. However, this inference is based on correlative TCGA data and does not imply a causal role in schistosomiasis-associated hepatocarcinogenesis.<\/jats:p>","DOI":"10.3390\/tropicalmed11060148","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T12:09:07Z","timestamp":1779970147000},"page":"148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Schistosoma japonicum Worms Alter the miRNA Expression Profile of Hepatic Stellate Cells with Potential Implications for Liver Fibrosis and Hepatocellular Carcinoma"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0967-9542","authenticated-orcid":false,"given":"Haoran","family":"Zhong","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Dong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danlin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhao","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhan","family":"Xiong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6903-3308","authenticated-orcid":false,"given":"Zhiqiang","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinming","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamei","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/S0140-6736(24)02814-9","article-title":"Human schistosomiasis","volume":"405","author":"Buonfrate","year":"2025","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e327","DOI":"10.1016\/S1473-3099(22)00221-3","article-title":"Review of 2022 WHO guidelines on the control and elimination of schistosomiasis","volume":"22","author":"Lo","year":"2022","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/s41572-018-0013-8","article-title":"Schistosomiasis","volume":"4","author":"McManus","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.curtheres.2019.06.001","article-title":"Schistosomiasis: Life Cycle, Diagnosis, and Control","volume":"91","author":"Nelwan","year":"2019","journal-title":"Curr. Ther. Res. Clin. Exp."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.pt.2013.12.009","article-title":"Cellular and chemokine-mediated regulation in schistosome-induced hepatic pathology","volume":"30","author":"Chuah","year":"2014","journal-title":"Trends Parasitol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/sj.icb.7100014","article-title":"Immunopathology of schistosomiasis","volume":"85","author":"Wilson","year":"2007","journal-title":"Immunol. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Reinholdt, C., Winkelmann, F., Koslowski, N., Reisinger, E.C., and Sombetzki, M. (2023). Unisexual infection with Schistosoma mansoni in mice has the potential to boost the immune response against eggs after challenge infection. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1125912"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1038\/nrmicro801","article-title":"Focus: Schistosomiasis","volume":"2","author":"Chitsulo","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.pt.2018.02.005","article-title":"Schistosome-Induced Fibrotic Disease: The Role of Hepatic Stellate Cells","volume":"34","author":"Carson","year":"2018","journal-title":"Trends Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1053\/hupa.2000.8440","article-title":"Hepatic stellate cell activation in nonalcoholic steatohepatitis and fatty liver","volume":"31","author":"Washington","year":"2000","journal-title":"Hum. Pathol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.ijpara.2006.04.015","article-title":"A contributory role for activated hepatic stellate cells in the dynamics of Schistosoma japonicum egg-induced fibrosis","volume":"36","author":"Bartley","year":"2006","journal-title":"Int. J. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"674","DOI":"10.4321\/S1130-01082006000900005","article-title":"Hepatic stellate cells and alcoholic liver disease","volume":"98","author":"Vera","year":"2006","journal-title":"Rev. Esp. Enferm. Dig."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Schwartz, C., and Fallon, P.G. (2018). Schistosoma \u201cEggs-Iting\u201d the Host: Granuloma Formation and Egg Excretion. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.02492"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","article-title":"MicroRNAs: Genomics, biogenesis, mechanism, and function","volume":"116","author":"Bartel","year":"2004","journal-title":"Cell"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1146\/annurev.cellbio.23.090506.123406","article-title":"microRNA functions","volume":"23","author":"Bushati","year":"2007","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.pt.2023.12.003","article-title":"The knowns and unknowns of helminth\u2013host miRNA cross-kingdom communication","volume":"40","author":"Chowdhury","year":"2024","journal-title":"Trends Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/1756-3305-7-148","article-title":"Expression of microRNA-454 in TGF-\u03b21-stimulated hepatic stellate cells and in mouse livers infected with Schistosoma japonicum","volume":"7","author":"Zhu","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1785738","DOI":"10.1080\/20013078.2020.1785738","article-title":"Sja-miR-71a in Schistosome egg-derived extracellular vesicles suppresses liver fibrosis caused by schistosomiasis via targeting semaphorin 4D","volume":"9","author":"Wang","year":"2020","journal-title":"J. Extracell. Vesicles"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"111523","DOI":"10.1016\/j.molbiopara.2022.111523","article-title":"MicroRNA-181b promotes schistosomiasis-induced hepatic fibrosis by targeting Smad7","volume":"252","author":"Wang","year":"2022","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1186\/s13071-025-06824-w","article-title":"MiR-383-5p promotes schistosomiasis-induced liver fibrosis by targeting peroxiredoxin-3","volume":"18","author":"Li","year":"2025","journal-title":"Parasites Vectors"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.pt.2022.02.011","article-title":"Host-parasite interactions mediated by cross-species microRNAs","volume":"38","author":"He","year":"2022","journal-title":"Trends Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.jhep.2019.10.029","article-title":"A schistosome miRNA promotes host hepatic fibrosis by targeting transforming growth factor beta receptor III","volume":"72","author":"He","year":"2020","journal-title":"J. Hepatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s40249-025-01298-5","article-title":"Reassessing Schistosoma worms: The overlooked role in host pathology and disease elimination","volume":"14","author":"Zhong","year":"2025","journal-title":"Infect. Dis. Poverty"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e11","DOI":"10.1017\/S0022149X24000762","article-title":"Can Schistosoma japonicum infection cause liver cancer?","volume":"99","author":"Jain","year":"2025","journal-title":"J. Helminthol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1002\/ueg2.12286","article-title":"New concepts in the treatment of hepatocellular carcinoma","volume":"10","author":"Sidali","year":"2022","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Knoll, L.J., Brindley, P.J., and Loukas, A. (2017). Helminth infection\u2013induced malignancy. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006393"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1017\/S0031182000086248","article-title":"The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms","volume":"55","author":"Smithers","year":"1965","journal-title":"Parasitology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Peterson, S.M., Thompson, J.A., Ufkin, M.L., Sathyanarayana, P., Liaw, L., and Congdon, C.B. (2014). Common features of microRNA target prediction tools. Front. Genet., 5.","DOI":"10.3389\/fgene.2014.00023"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1038\/ng.2764","article-title":"The Cancer Genome Atlas Pan-Cancer analysis project","volume":"45","author":"Weinstein","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhong, H., Dong, B., Zhu, D., Fu, Z., Liu, J., Guan, G., and Jin, Y. (2024). Schistosoma japonicum sja-let-7 Inhibits the Growth of Hepatocellular Carcinoma Cells via Cross-Species Regulation of Col1\u03b12. Genes, 15.","DOI":"10.3390\/genes15091165"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"von B\u00fclow, V., Lichtenberger, J., Grevelding, C.G., Falcone, F.H., Roeb, E., and Roderfeld, M. (2021). Does Schistosoma Mansoni Facilitate Carcinogenesis?. Cells, 10.","DOI":"10.3390\/cells10081982"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Zhong, H., Dong, B., Zhu, D., Fu, Z., Liu, J., and Jin, Y. (2024). Sja-let-7 suppresses the development of liver fibrosis via Schistosoma japonicum extracellular vesicles. PLoS Pathog., 20.","DOI":"10.1371\/journal.ppat.1012153"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Sombetzki, M., Reinholdt, C., Winkelmann, F., Rabes, A., Koslowski, N., and Reisinger, E.C. (2022). A one-year unisexual Schistosoma mansoni infection causes pathologic organ alterations and persistent non-polarized T cell-mediated inflammation in mice. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1010932"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Sombetzki, M., Koslowski, N., Rabes, A., Seneberg, S., Winkelmann, F., Fritzsche, C., Loebermann, M., and Reisinger, E.C. (2018). Host Defense Versus Immunosuppression: Unisexual Infection With Male or Female Schistosoma mansoni Differentially Impacts the Immune Response Against Invading Cercariae. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.00861"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Lu, D.B., Deng, Y., Ding, H., Liang, Y.S., and Webster, J.P. (2018). Single-sex schistosome infections of definitive hosts: Implications for epidemiology and disease control in a changing world. PLoS Pathog., 14.","DOI":"10.1371\/journal.ppat.1006817"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"107747","DOI":"10.1016\/j.actatropica.2025.107747","article-title":"RNA sequencing analysis reveals the impact of Schistosoma japonicum worm on host liver fibrosis","volume":"270","author":"Zhong","year":"2025","journal-title":"Acta Trop."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zhong, H., and Jin, Y. (2023). Single-sex schistosomiasis: A mini review. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1158805"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Anthony, B.J., James, K.R., Gobert, G.N., Ramm, G.A., and McManus, D.P. (2013). Schistosomajaponicum Eggs Induce a Proinflammatory, Anti-Fibrogenic Phenotype in Hepatic Stellate Cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0068479"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ijpara.2013.11.003","article-title":"Schistosoma japonicum soluble egg antigens induce apoptosis and inhibit activation of hepatic stellate cells: A possible molecular mechanism","volume":"44","author":"Duan","year":"2014","journal-title":"Int. J. Parasitol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Duan, Y., Pan, J., Chen, J., Zhu, D., Wang, J., Sun, X., Chen, L., and Wu, L. (2016). Soluble Egg Antigens of Schistosoma japonicum Induce Senescence of Activated Hepatic Stellate Cells by Activation of the FoxO3a\/SKP2\/P27 Pathway. PLoS Neglected Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0005268"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"41159","DOI":"10.1038\/s41598-025-26188-6","article-title":"Bioinformatics analysis to explore the potential prognostic utility of hsa-miR-103a-3p in head and neck squamous cell carcinoma","volume":"15","author":"Shoukry","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"104274","DOI":"10.1016\/j.labinv.2025.104274","article-title":"Comprehensive Transcriptome and miRNome Profiling in Metachronous Colorectal Liver Metastasis: Insight Into the Prognostic and Molecular Subtypes","volume":"106","author":"Rao","year":"2026","journal-title":"Lab. Investig."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"537","DOI":"10.3892\/etm.2022.11474","article-title":"miR-146b-5p activation of hepatic stellate cells contributes to the progression of fibrosis by directly targeting HIPK1","volume":"24","author":"Xie","year":"2022","journal-title":"Exp. Ther. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"28683","DOI":"10.18632\/oncotarget.15640","article-title":"Down-regulation of miR-146b-5p by long noncoding RNA MALAT1 in hepatocellular carcinoma promotes cancer growth and metastasis","volume":"8","author":"Li","year":"2017","journal-title":"Oncotarget"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gupta, P., Sata, T.N., Yadav, A.K., Mishra, A., Vats, N., Hossain, M.M., Sanal, M.G., and Venugopal, S.K. (2019). TGF-\u03b2 induces liver fibrosis via miRNA-181a-mediated down regulation of augmenter of liver regeneration in hepatic stellate cells. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0214534"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"215","DOI":"10.3892\/ijmm.2021.5048","article-title":"MALAT1 promotes liver fibrosis by sponging miR-181a and activating TLR4-NF-\u03baB signaling","volume":"48","author":"Wang","year":"2021","journal-title":"Int. J. Mol. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"10731","DOI":"10.1007\/s13277-014-2018-6","article-title":"miR-24 promotes the proliferation and invasion of HCC cells by targeting SOX7","volume":"35","author":"Ma","year":"2014","journal-title":"Tumour Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6180","DOI":"10.1038\/s41388-021-02011-0","article-title":"miR-151a-3p-rich small extracellular vesicles derived from gastric cancer accelerate liver metastasis via initiating a hepatic stemness-enhancing niche","volume":"40","author":"Li","year":"2021","journal-title":"Oncogene"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1186\/s12935-025-04055-8","article-title":"Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy","volume":"25","author":"Shu","year":"2025","journal-title":"Cancer Cell Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e47882","DOI":"10.15252\/embr.201947882","article-title":"Schistosomal extracellular vesicle-enclosed miRNAs modulate host T helper cell differentiation","volume":"21","author":"Meningher","year":"2020","journal-title":"EMBO Rep."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/11\/6\/148\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T04:29:05Z","timestamp":1780115345000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/11\/6\/148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,28]]},"references-count":53,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["tropicalmed11060148"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed11060148","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,28]]}}}