{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:36:39Z","timestamp":1785429399715,"version":"3.56.0"},"reference-count":32,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa no Estado do Rio de Janeiro-FAPERJ Process","award":["E- 26\/210.697\/2024"],"award-info":[{"award-number":["E- 26\/210.697\/2024"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa no Estado do Rio de Janeiro-FAPERJ Process","award":["E-26\/204.542\/2022"],"award-info":[{"award-number":["E-26\/204.542\/2022"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304322\/2025-3"],"award-info":[{"award-number":["304322\/2025-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"award":["304322\/2025-3"],"award-info":[{"award-number":["304322\/2025-3"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Trop. Med. Infect. Dis.","TropicalMed"],"abstract":"<jats:p>Cutaneous leishmaniasis (CL) is a vector-borne disease caused by Leishmania spp. Molecular methods such as conventional PCR (cPCR) and real-time PCR (qPCR) show high accuracy but remain restricted to reference centers. Loop-mediated isothermal amplification (LAMP) enables rapid DNA amplification under isothermal conditions. LAMP targeting 18S rDNA using WarmStart\u00ae Colorimetric LAMP 2\u00d7 Master Mix and Bst 2.0 DNA Polymerase with SYBR\u00ae Green I (BstSg) was evaluated in swab samples (extracted by kit\u2014SW-kit and direct boil\u2014SW-DB) and specimens collected through biopsy from ulcerated lesions of patients attending a reference center in Rio de Janeiro, Brazil. The reference standard was defined as at least one positive parasitological or molecular (cPCR) test plus clinical confirmation. Analytical sensitivity reached 10 fg with WS and 10 pg with BstSg. The diagnostic accuracy of LAMP ranged from 51.8% to 78.6%, with sensitivity from 52.5% to 81% and specificity from 39.6% to 89.4%. LAMP, particularly when applied to swab samples with DNA extracted using the SW-kit protocol and amplified with BstSg, showed promising diagnostic accuracy and operational feasibility as a less invasive approach for CL diagnosis, eliminating the need for skin biopsy and reducing patient discomfort during sample collection.<\/jats:p>","DOI":"10.3390\/tropicalmed11070207","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:20:39Z","timestamp":1784712039000},"page":"207","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Loop-Mediated Isothermal Amplification (LAMP) Technique in Swab Samples from Ulcerated Cutaneous Lesions Compared with Conventional Diagnostic Methods for American Tegumentary Leishmaniasis in Patients Treated at a Reference Center in Rio de Janeiro, Brazil"],"prefix":"10.3390","volume":"11","author":[{"given":"Elizabeth Cristina Ara\u00fajo Ferreira","family":"Faulhaber","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7907-7913","authenticated-orcid":false,"given":"Daniel Moreira de","family":"Avelar","sequence":"additional","affiliation":[{"name":"Grupo de Estudos em Leishmanioses, Instituto Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Belo Horizonte 30190-002, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2714-7139","authenticated-orcid":false,"given":"Liliane de F. Antonio","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia Cl\u00ednica, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-1922","authenticated-orcid":false,"given":"Luciana de F. Campos","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia em Leishmanioses, Instituto Carlos Chagas, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Curitiba 81350-010, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cintia Xavier","family":"de Mello","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Interdisciplinar de Pesquisas M\u00e9dicas, Instituto Oswaldo Cruz (IOC), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle Baldan","family":"Stanciola","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luanna da S.","family":"Ventura","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-2234","authenticated-orcid":false,"given":"Marcelo Rosandiski","family":"Lyra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8120-1864","authenticated-orcid":false,"given":"Caio Thomaz de Lima","family":"e Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"},{"name":"Laborat\u00f3rio Interdisciplinar de Pesquisas M\u00e9dicas, Instituto Oswaldo Cruz (IOC), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7345-0350","authenticated-orcid":false,"given":"Aline Fagundes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosely Maria","family":"Zancope-Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-0502","authenticated-orcid":false,"given":"Maria In\u00eas Fernandes","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7197-2128","authenticated-orcid":false,"given":"Andreza Pain","family":"Marcelino","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas (INI), Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2026, June 26). 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