{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:11:23Z","timestamp":1760242283990,"version":"build-2065373602"},"reference-count":14,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,4,7]],"date-time":"2017-04-07T00:00:00Z","timestamp":1491523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001792","name":"James Cook University","doi-asserted-by":"publisher","award":["H5261"],"award-info":[{"award-number":["H5261"]}],"id":[{"id":"10.13039\/501100001792","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Medical Research, Myanmar Ministry of Health and Sports"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Diagnostic testing for the antibody Bm14 is used to assess the prevalence of bancroftian and brugian filariasis in endemic populations. Using dried blood spots (DBS) collected on filter paper is ideal in resource-poor settings, but concerns have been raised about the performance of DBS samples compared to plasma or serum. In addition, two versions of the test have been used: the Bm14 CELISA (Cellabs Pty Ltd., Manly, Australia) or an in-house CDC version. Due to recent improvements in the CELISA, it is timely to validate the latest versions of the Bm14 ELISA for both plasma and DBS, especially in settings of residual infection with low antibody levels. We tested plasma and DBS samples taken simultaneously from 92 people in Myanmar, of whom 37 (40.2%) were positive in a rapid antigen test. Comparison of results from plasma and DBS samples demonstrated no significant difference in positive proportions using both the CELISA (46.7% and 44.6%) and CDC ELISA (50.0% and 47.8%). Quantitative antibody unit results from each sample type were also highly correlated, with coefficients &gt;0.87. The results of this study demonstrate that DBS samples are a valid collection strategy and give equivalent results to plasma for Bm14 antibody ELISA testing by either test type.<\/jats:p>","DOI":"10.3390\/tropicalmed2020006","type":"journal-article","created":{"date-parts":[[2017,4,7]],"date-time":"2017-04-07T10:49:53Z","timestamp":1491562193000},"page":"6","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Concordance between Plasma and Filter Paper Sampling Techniques for the Lymphatic Filariasis Bm14 Antibody ELISA"],"prefix":"10.3390","volume":"2","author":[{"given":"Jesse","family":"Masson","sequence":"first","affiliation":[{"name":"College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3097-3132","authenticated-orcid":false,"given":"Jan","family":"Douglass","sequence":"additional","affiliation":[{"name":"College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen","family":"Roineau","sequence":"additional","affiliation":[{"name":"College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khin","family":"Aye","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Myanmar Ministry of Health and Sports, Nay Pyi Taw, Myanmar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyi","family":"Htwe","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Myanmar Ministry of Health and Sports, Nay Pyi Taw, Myanmar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Warner","sequence":"additional","affiliation":[{"name":"College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5215-3901","authenticated-orcid":false,"given":"Patricia","family":"Graves","sequence":"additional","affiliation":[{"name":"College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Simonsen, P.E., Pedersen, E.M., Rwegoshora, R.T., Malecela, M.N., Derua, Y.A., and Magesa, S.M. 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