{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T21:15:28Z","timestamp":1788902128806,"version":"build-2803163510"},"reference-count":18,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2017,7,9]],"date-time":"2017-07-09T00:00:00Z","timestamp":1499558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007537","name":"Agricultural Research Council","doi-asserted-by":"publisher","award":["P10000029"],"award-info":[{"award-number":["P10000029"]}],"id":[{"id":"10.13039\/100007537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>The aetiological agent of rabies is a member of the Lyssavirus genus (Rhabdoviridae family, order Mononegavirales). The disease (rabies) is endemic in many parts of Asia and Africa and still remains an important public and veterinary health threat. In Nigeria, there is a dearth of information on the natural infection and\/or exposure of bat species to lyssaviruses. Therefore, this study was undertaken to assess the prevalence of rabies virus (RABV) neutralizing antibodies in sera obtained from bats from the central Plateau and North-East Bauchi States in Nigeria. Two hundred serum samples were collected from Nigerian fruit bats from six different locations and tested for anti-RABV antibodies using a commercial blocking ELISA. Of the 200 bat serum samples collected, one batch consisting of 111 samples did not meet the validation criteria and hence was not included in the final analysis. Of the remaining 89, only three (3.4%) contained anti-lyssavirus antibodies, demonstrating a low prevalence of lyssavirus antibodies in the study population. In order to further understand the exposure of bat species to phylogroup II lyssaviruses (Lagos bat virus and Mokola virus), the same panel of samples will be tested for neutralizing antibodies to phylogroup II members, viruses that do not cross-neutralize with members of phylogroup I.<\/jats:p>","DOI":"10.3390\/tropicalmed2030026","type":"journal-article","created":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T11:05:31Z","timestamp":1499684731000},"page":"26","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sero-Surveillance of Lyssavirus Specific Antibodies in Nigerian Fruit Bats (Eidolon helvum)"],"prefix":"10.3390","volume":"2","author":[{"given":"Dinchi","family":"Tyem","sequence":"first","affiliation":[{"name":"National Veterinary Research Institute, P.M.B. 1, Vom-Jos Plateau State, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banenat","family":"Dogonyaro","sequence":"additional","affiliation":[{"name":"National Veterinary Research Institute, P.M.B. 1, Vom-Jos Plateau State, Nigeria"},{"name":"University of Pretoria, Faculty of Veterinary Sciences, Department of Veterinary Tropical Diseases, P Bag X04, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Woma","sequence":"additional","affiliation":[{"name":"National Veterinary Research Institute, P.M.B. 1, Vom-Jos Plateau State, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3272-9547","authenticated-orcid":false,"given":"Ernest","family":"Ngoepe","sequence":"additional","affiliation":[{"name":"OIE Rabies Reference Laboratory, Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7842-7985","authenticated-orcid":false,"given":"Claude","family":"Sabeta","sequence":"additional","affiliation":[{"name":"University of Pretoria, Faculty of Veterinary Sciences, Department of Veterinary Tropical Diseases, P Bag X04, Onderstepoort 0110, South Africa"},{"name":"OIE Rabies Reference Laboratory, Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,7,9]]},"reference":[{"key":"ref_1","unstructured":"(2017, May 30). ICTV (International Committee on Taxonomy of Viruses). Available online: http:\/\/www.ictvonline.org\/virusTaxonomy.asp?bhcp=1."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e01427\u201316","DOI":"10.1128\/genomeA.01427-16","article-title":"Complete genome sequence of Lleida bat lyssavirus","volume":"5","author":"Marston","year":"2017","journal-title":"Genome Announc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"793","DOI":"10.3201\/eid1905.121071","article-title":"Novel lyssavirus in bat, Spain","volume":"19","author":"Berciano","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.3201\/eid2208.151986","article-title":"Lyssavirus in Indian flying foxes, Sri Lanka","volume":"22","author":"Gunawardena","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3268","DOI":"10.1128\/JVI.75.7.3268-3276.2001","article-title":"Evidence of two Lyssavirus phylogroups with distinct pathogenicity and immunogenicity","volume":"75","author":"Badrane","year":"2001","journal-title":"J. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.virusres.2005.03.009","article-title":"Efficacy of rabies biologics against new lyssaviruses from Eurasia","volume":"111","author":"Hanlon","year":"2005","journal-title":"Virus Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.virusres.2010.01.018","article-title":"Shimoni bat virus, a new representative of the Lyssavirus genus","volume":"149","author":"Kuzmin","year":"2010","journal-title":"Virus Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Hayman, D.T.S., Fooks, A.R., Marston, D.A., and Garcia-R, J.C. (2016). The Global Phylogeography of Lyssaviruses\u2014Challenging the \u2018Out of Africa\u2019 hypothesis. PLoS Neglect. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0005266"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1016\/S0140-6736(13)62707-5","article-title":"Current status of rabies and prospects for elimination","volume":"384","author":"Fooks","year":"2014","journal-title":"Lancet"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Hampson, K., Coudeville, L., Lembo, T., Sambo, M., Kieffer, A., Attlan, M., Barrat, J., Blanton, J.D., Briggs, D.J., and Cleaveland, S. (2015). Estimating the global burden of endemic canine rabies. PLoS Neglect. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003786"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/0035-9203(58)90127-5","article-title":"Isolation of a virus from Nigerian fruit bats","volume":"52","author":"Boulger","year":"1958","journal-title":"Trans. Roy. Soc. Trop. Med. Hyg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"267","DOI":"10.7589\/0090-3558-46.1.267","article-title":"Evidence of Lagos bat virus circulation among Nigerian fruit bats","volume":"46","author":"Dzikwi","year":"2010","journal-title":"J. Wildl. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1017\/S0950268812000167","article-title":"Endemic Lagos bat virus infection in Eidolon helvum","volume":"140","author":"Hayman","year":"2012","journal-title":"Epidemiol. Infect."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1128\/JCM.00016-08","article-title":"Lagos bat virus in Kenya","volume":"46","author":"Kuzmin","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1590\/S0037-86822011005000011","article-title":"Rabies diagnosis and serology in bats from the State of S\u00e3o Paulo, Brazil","volume":"44","author":"Amatuzzi","year":"2011","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jviromet.2016.10.006","article-title":"First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe","volume":"238","author":"Wasniewski","year":"2016","journal-title":"J. Virol. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0022-1759(97)00212-3","article-title":"Development of a fluorescent antibody virus neutralisation test (FAVN test) for the quantitation of rabies-neutralising antibody","volume":"212","author":"Cliquet","year":"1998","journal-title":"J. Immunol. Methods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"258","DOI":"10.7589\/0090-3558-26.2.258","article-title":"Detection of rabies virus antibodies in fruit bats (Eidolon helvum) from Nigeria","volume":"26","author":"Aghomo","year":"1990","journal-title":"J. Wildl. Dis."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/2\/3\/26\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:42:03Z","timestamp":1760208123000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/2\/3\/26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,9]]},"references-count":18,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2017,9]]}},"alternative-id":["tropicalmed2030026"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed2030026","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,9]]}}}