{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T03:59:48Z","timestamp":1762142388336,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T00:00:00Z","timestamp":1507075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["605804","Public Health Research and Development Committee"],"award-info":[{"award-number":["605804","Public Health Research and Development Committee"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>(1) Background: soil-transmitted helminths are a problem worldwide, largely affecting disadvantaged populations. The little data available indicates high rates of infection in some remote Aboriginal communities in Australia. Studies of helminths were carried out in the same remote community in the Northern Territory in 1994\u20131996 and 2010\u20132011; (2) Methods: fecal samples were collected from children aged &lt;10 years and examined for helminths by direct smear microscopy. In the 2010\u20132011 study, some fecal samples were also analyzed by agar plate culture and PCR for Strongyloides stercoralis DNA. Serological analysis of fingerprick dried blood spots using a S. stercoralis NIE antigen was also conducted; (3) Results and Conclusions: a reduction in fecal samples positive for S. stercoralis, hookworm and Trichuris trichiura was seen between the studies in 1994\u20131996 and 2010\u20132011, likely reflecting public health measures undertaken in the region to reduce intestinal helminths. Comparison of methods to detect S. stercoralis showed that PCR of fecal samples and serological testing of dried blood spots was at least as sensitive as direct smear microscopy and agar plate culture. These methods have advantages for use in remote field studies.<\/jats:p>","DOI":"10.3390\/tropicalmed2040051","type":"journal-article","created":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T13:53:55Z","timestamp":1507125235000},"page":"51","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Soil-Transmitted Helminths in Children in a Remote Aboriginal Community in the Northern Territory: Hookworm is Rare but Strongyloides stercoralis and Trichuris trichiura Persist"],"prefix":"10.3390","volume":"2","author":[{"given":"Deborah","family":"Holt","sequence":"first","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin NT 0811, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9270-2493","authenticated-orcid":false,"given":"Jennifer","family":"Shield","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin NT 0811, Australia"},{"name":"Department of Pharmacy and Applied Science, La Trobe University, Bendigo VIC 3550, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9312-7451","authenticated-orcid":false,"given":"Tegan","family":"Harris","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin NT 0811, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate","family":"Mounsey","sequence":"additional","affiliation":[{"name":"School of Health and Sports Science, University of the Sunshine Coast, Maroochydore QLD 4558, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kieran","family":"Aland","sequence":"additional","affiliation":[{"name":"Queensland Museum, South Brisbane QLD 4101, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"McCarthy","sequence":"additional","affiliation":[{"name":"QIMR Berghofer Medical Research Institute, Herston QLD 4006, Australia"},{"name":"School of Medicine, University of Queensland, Brisbane QLD 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8878-8837","authenticated-orcid":false,"given":"Bart","family":"Currie","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin NT 0811, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0862-7572","authenticated-orcid":false,"given":"Therese","family":"Kearns","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin NT 0811, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,10,4]]},"reference":[{"key":"ref_1","unstructured":"(2017, July 17). World Health Organization. Available online: http:\/\/www.who.int\/mediacentre\/factsheets\/fs366\/en\/."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.5694\/j.1326-5377.1997.tb140103.x","article-title":"The prevalence of hookworm infection, iron deficiency and anaemia in an aboriginal community in north-west Australia","volume":"166","author":"Hopkins","year":"1997","journal-title":"Med. J. Aust."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"44","DOI":"10.5365\/wpsar.2015.6.1.008","article-title":"Intestinal parasites of children and adults in a remote Aboriginal community of the Northern Territory, Australia, 1994\u20131996","volume":"6","author":"Shield","year":"2015","journal-title":"Western Pac. Surveill. Response J."},{"key":"ref_4","first-page":"20","article-title":"Pilot screening program for intestinal parasites and anaemia in adults in a Top End Aboriginal community","volume":"4","author":"Fryar","year":"1997","journal-title":"NT Comm Dis Bull."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/j.1440-1584.2005.00710.x","article-title":"Strongyloidiasis: A review of the evidence for Australian practitioners","volume":"13","author":"Johnston","year":"2005","journal-title":"Aust. J. Rural Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e0005607","DOI":"10.1371\/journal.pntd.0005607","article-title":"Strongyloides seroprevalence before and after an ivermectin mass drug administration in a remote Australian Aboriginal community","volume":"11","author":"Kearns","year":"2017","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1086\/322707","article-title":"Diagnosis of Strongyloides stercoralis infection","volume":"33","author":"Siddiqui","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"ref_8","first-page":"264","article-title":"Strongyloides serology \u2013 useful for diagnosis and management of strongyloidiasis in rural Indigenous populations, but important gaps in knowledge remain","volume":"4","author":"Speare","year":"2004","journal-title":"Rural Remote Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0065-308X(08)60036-6","article-title":"Human strongyloidiasis","volume":"38","author":"Grove","year":"1996","journal-title":"Adv. Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1128\/jcm.34.10.2569-2571.1996","article-title":"Patterns of detection of Strongyloides stercoralis in stool specimens: Implications for diagnosis and clinical trials","volume":"34","author":"Dreyer","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","first-page":"159","article-title":"Experimental and epidemiological studies on strongyloidiasis of Amami Oshima island","volume":"28","author":"Tanaka","year":"1958","journal-title":"Jpn. J. Exp. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e0004151","DOI":"10.1371\/journal.pntd.0004151","article-title":"Impact of an ivermectin mass drug administration on scabies prevalence in a remote Australian Aboriginal community","volume":"9","author":"Kearns","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref_13","unstructured":"(2017, August 31). Australian Bureau of Statistics, Available online: http:\/\/www.censusdata.abs.gov.au\/census_services\/getproduct\/census\/2016\/quickstat\/SSC70106."},{"key":"ref_14","unstructured":"(2017, September 30). VassarStats: Website for Statistical Computation. Available online: http:\/\/vassarstats.net\/."},{"key":"ref_15","unstructured":"Garcia, L. (2007). Diagnostic Medical Parasitology, American Society for Microbiology Press. [5th ed.]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.4269\/ajtmh.12-0437","article-title":"Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool","volume":"88","author":"Sultana","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.trstmh.2008.12.001","article-title":"Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR","volume":"103","author":"Verweij","year":"2009","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0166-6851(02)00214-1","article-title":"Characterization of a recombinant immunodiagnostic antigen (NIE) from Strongyloides stercoralis L3-stage larvae","volume":"125","author":"Ravi","year":"2002","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.actatropica.2014.07.007","article-title":"Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE","volume":"138","author":"Mounsey","year":"2014","journal-title":"Acta Trop."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1093\/ije\/dyn240","article-title":"Burden of disease and injury in Aboriginal and Torres Strait Islander Peoples: the Indigenous health gap","volume":"38","author":"Vos","year":"2009","journal-title":"Int. J. Epidemiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1136\/jech.2009.091637","article-title":"Evaluation of an Australian indigenous housing programme: Community level impact on crowding, infrastructure function and hygiene","volume":"65","author":"Bailie","year":"2011","journal-title":"J. Epidemiol. Community Health"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1136\/jech.2011.134874","article-title":"The impact of housing improvement and socio-environmental factors on common childhood illnesses: A cohort study in Indigenous Australian communities","volume":"66","author":"Bailie","year":"2012","journal-title":"J. Epidemiol. Community Health"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/j.1440-1754.2010.01846.x","article-title":"Hygiene improvement: Essential to improving child health in remote Aboriginal communities","volume":"46","author":"McDonald","year":"2010","journal-title":"J. Paediatr. Child. Health"},{"key":"ref_24","unstructured":"Central Australian Rural Practitioners Association (1994). CARPA Standard Treatment Manual, Central Australian Rural Practitioners Association. [2nd ed.]."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"278","DOI":"10.5694\/mja12.11615","article-title":"Hookworm in the Northern Territory: down but not out","volume":"198","author":"Davies","year":"2013","journal-title":"Med. J. Aust."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3390\/tropicalmed2020018","article-title":"Trends in Strongyloides stercoralis faecal larvae detections in the Northern Territory, Australia: 2002\u20132012","volume":"2","author":"Crowe","year":"2017","journal-title":"Trop. Med. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"286","DOI":"10.5694\/mja13.00141","article-title":"Decreasing prevalence of Trichuris trichiura (whipworm) in the Northern Territory from 2002 to 2012","volume":"200","author":"Crowe","year":"2014","journal-title":"Med. J. Aust."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"S113","DOI":"10.1017\/S0031182000007290","article-title":"Albendazole: A review of anthelmintic efficacy and safety in humans","volume":"121","author":"Horton","year":"2000","journal-title":"Parasitology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1001\/jama.299.16.1937","article-title":"Efficacy of current drugs against soil-transmitted helminth infections: systematic review and meta-analysis","volume":"299","author":"Keiser","year":"2008","journal-title":"J.A.M.A."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e25003","DOI":"10.1371\/journal.pone.0025003","article-title":"Efficacy of single-dose and triple-dose albendazole and mebendazole against soil-transmitted helminths and Taenia spp.: A randomized controlled trial","volume":"6","author":"Steinmann","year":"2011","journal-title":"PLoS One"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1111\/jpc.12885","article-title":"Dwarf tapeworm (Hymenolepis nana): Characteristics in the Northern Territory 2002\u20132013","volume":"51","author":"Willcocks","year":"2015","journal-title":"J. Paediatr. Child. Health"},{"key":"ref_32","unstructured":"Central Australian Rural Practitioners Association (2014). CARPA Standard Treatment Manual, Central Australian Rural Practitioners Association. [5th ed.]."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"248","DOI":"10.4269\/ajtmh.1995.53.248","article-title":"Efficacy of stool examination for detection of Strongyloides infection","volume":"53","author":"Sato","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Beknazarova, M., Millsteed, S., Robertson, G., Whiley, H., and Ross, K. (2017). Validation of DESS as a DNA preservation method for the detection of Strongyloides spp. in canine feces. Int. J. Environ. Res. Public Health.","DOI":"10.3390\/ijerph14060624"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"306","DOI":"10.4269\/ajtmh.13-0583","article-title":"A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye","volume":"90","author":"Watts","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Fernandez-Soto, P., Sanchez-Hernandez, A., Gandasegui, J., Bajo Santos, C., Lopez-Aban, J., Saugar, J.M., Rodriguez, E., Vicente, B., and Muro, A. (2016). Strong-LAMP: A LAMP Assay for Strongyloides spp. detection in stool and urine samples. Towards the diagnosis of human strongyloidiasis starting from a rodent model. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004836"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.4269\/ajtmh.12-0726","article-title":"A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations","volume":"88","author":"Mejia","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"338","DOI":"10.4269\/ajtmh.2011.10-0499","article-title":"A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths","volume":"84","author":"Basuni","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Llewellyn, S., Inpankaew, T., Nery, S.V., Gray, D.J., Verweij, J.J., Clements, A.C., Gomes, S.J., Traub, R., and McCarthy, J.S. (2016). Application of a multiplex quantitative PCR to assess prevalence and intensity of intestinal parasite infections in a controlled clinical trial. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004380"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Pilotte, N., Papaiakovou, M., Grant, J.R., Bierwert, L.A., Llewellyn, S., McCarthy, J.S., and Williams, S.A. (2016). Improved PCR-based detection of soil transmitted helminth infections using a next-generation sequencing approach to assay design. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004578"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1017\/S0031182016002559","article-title":"A retrospective study comparing agar plate culture, indirect immunofluorescence and real-time PCR for the diagnosis of Strongyloides stercoralis infection","volume":"144","author":"Buonfrate","year":"2017","journal-title":"Parasitology"}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/2\/4\/51\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:46:35Z","timestamp":1760208395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/2\/4\/51"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,4]]},"references-count":41,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["tropicalmed2040051"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed2040051","relation":{},"ISSN":["2414-6366"],"issn-type":[{"type":"electronic","value":"2414-6366"}],"subject":[],"published":{"date-parts":[[2017,10,4]]}}}