{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:16:19Z","timestamp":1761218179407,"version":"build-2065373602"},"reference-count":21,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T00:00:00Z","timestamp":1558569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This work was carried out as part of a kick-starting project supported by the Swiss-African Research Cooperation (SARECO; University of Basel, Basel, Switzerland)","award":["-"],"award-info":[{"award-number":["-"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Intestinal parasite infections are frequent causes of diarrhea and malnutrition among children in the tropics. Transmission of helminths and intestinal protozoa is intimately connected with conditions of poverty, including inadequate sanitation and hygiene. Concurrent infections with several intestinal pathogens may lead to excess morbidity. Yet, there is a paucity of epidemiological data from Mali. In this study, stool samples from 56 individuals, aged 2\u201363 years, from Bamako and Niono, south-central Mali were examined for intestinal parasites using stool microscopy. Additionally, stool samples were subjected to a rapid diagnostic test (RDT) and polymerase chain reaction (PCR) for the detection of Cryptosporidium spp. and Giardia intestinalis. The predominant pathogens were Schistosoma mansoni and G. intestinalis with prevalences of 41% and 38%, respectively. Hymenolepis nana was detected in 4% of the participants, while no eggs of soil-transmitted helminths were found. Concurrent infections with G. intestinalis and S. mansoni were diagnosed in 16% of the participants. For the detection of G. intestinalis, PCR was more sensitive (100%) than RDT (62%) and microscopy (48%). As helminth-protozoa coinfections might have important implications for morbidity control programs, future studies should employ diagnostic tools beyond stool microscopy to accurately assess the co-endemicity of giardiasis and schistosomiasis.<\/jats:p>","DOI":"10.3390\/tropicalmed4020086","type":"journal-article","created":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T11:20:46Z","timestamp":1558696846000},"page":"86","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Prevalence of Giardia intestinalis Infection in Schistosomiasis-Endemic Areas in South-Central Mali"],"prefix":"10.3390","volume":"4","author":[{"given":"Hassan K.M.","family":"Fofana","sequence":"first","affiliation":[{"name":"Institut National de Recherche en Sant\u00e9 Publique, B.P. 1771, Bamako, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maren","family":"Schwarzkopf","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mama N.","family":"Doumbia","sequence":"additional","affiliation":[{"name":"Institut National de Recherche en Sant\u00e9 Publique, B.P. 1771, Bamako, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9nion","family":"Saye","sequence":"additional","affiliation":[{"name":"Institut National de Recherche en Sant\u00e9 Publique, B.P. 1771, Bamako, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Nimmesgern","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aly","family":"Landour\u00e9","sequence":"additional","affiliation":[{"name":"Institut National de Recherche en Sant\u00e9 Publique, B.P. 1771, Bamako, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamadou S.","family":"Traor\u00e9","sequence":"additional","affiliation":[{"name":"Institut National de Recherche en Sant\u00e9 Publique, B.P. 1771, Bamako, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1458-8306","authenticated-orcid":false,"given":"Pascal","family":"Mertens","sequence":"additional","affiliation":[{"name":"Coris BioConcept, 5032 Gembloux, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg","family":"Utzinger","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, P.O. Box, CH\u20134002 Basel, Switzerland"},{"name":"University of Basel, P.O. Box, CH\u20134003 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moussa","family":"Sacko","sequence":"additional","affiliation":[{"name":"Institut National de Recherche en Sant\u00e9 Publique, B.P. 1771, Bamako, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3634-8802","authenticated-orcid":false,"given":"S\u00f6ren L.","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Saarland University, 66421 Homburg\/Saar, Germany"},{"name":"Swiss Tropical and Public Health Institute, P.O. Box, CH\u20134002 Basel, Switzerland"},{"name":"University of Basel, P.O. Box, CH\u20134003 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Becker, S.L., Vogt, J., Knopp, S., Panning, M., Warhurst, D.C., Polman, K., Marti, H., von M\u00fcller, L., Yansouni, C.P., and Jacobs, J. (2013). Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests. BMC Infect. Dis., 13.","DOI":"10.1186\/1471-2334-13-37"},{"key":"ref_2","unstructured":"Polman, K., Becker, S.L., Alirol, E., Bhatta, N.K., Bhattarai, N.R., Bottieau, E., Bratschi, M.W., Burza, S., Coulibaly, J.T., and Doumbia, M.N. (2015). Diagnosis of neglected tropical diseases among patients with persistent digestive disorders (diarrhoea and\/or abdominal pain \u226514 days): a multi-country, prospective, non-experimental case-control study. BMC Infect. 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