{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T04:48:58Z","timestamp":1781239738529,"version":"3.54.1"},"reference-count":94,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH International Collaborations in Infectious Disease Research grant","award":["U01 AI115577"],"award-info":[{"award-number":["U01 AI115577"]}]},{"name":"Students Training in Advanced Research","award":["NIH T35 OD010956"],"award-info":[{"award-number":["NIH T35 OD010956"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Land cover and land use change (LCLUC) acts as a catalyst for spillover of arthropod-borne pathogens into novel hosts by shifting host and vector diversity, abundance, and distribution, ultimately reshaping host\u2013vector interactions. Identification of bloodmeals from wild-caught mosquitoes provides insight into host utilization of particular species in particular land cover types, and hence their potential role in pathogen maintenance and spillover. Here, we collected 134 blood-engorged mosquitoes comprising 10 taxa across 9 land cover types in Sarawak, Malaysian Borneo, a region experiencing intense LCLUC and concomitant spillover of arthropod-borne pathogens. Host sources of blood were successfully identified for 116 (87%) mosquitoes using cytochrome oxidase subunit I (COI) barcoding. A diverse range of hosts were identified, including reptiles, amphibians, birds, and mammals. Sixteen engorged Aedes albopictus, a major vector of dengue virus, were collected from seven land cover types and found to feed exclusively on humans (73%) and boar (27%). Culex tritaeniohynchus (n = 2), Cx. gelidus (n = 3), and Cx. quiquefasciatus (n = 3), vectors of Japanese encephalitis virus, fed on humans and pigs in the rural built-up land cover, creating potential transmission networks between these species. Our data support the use of COI barcoding to characterize mosquito\u2013host networks in a biodiversity hotspot.<\/jats:p>","DOI":"10.3390\/tropicalmed5020051","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T11:57:14Z","timestamp":1585828634000},"page":"51","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Identification of Mosquito Bloodmeals Collected in Diverse Habitats in Malaysian Borneo Using COI Barcoding"],"prefix":"10.3390","volume":"5","author":[{"given":"Katherine I.","family":"Young","sequence":"first","affiliation":[{"name":"Department of Biology, New Mexico State University, Las Cruces NM 88003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph T.","family":"Medwid","sequence":"additional","affiliation":[{"name":"Department of Biology, New Mexico State University, Las Cruces NM 88003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9182-216X","authenticated-orcid":false,"given":"Sasha R.","family":"Azar","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Institute for Translational Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Huff","sequence":"additional","affiliation":[{"name":"Department of Biology, Baylor University, Waco, TX 76706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Drumm","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0718-5146","authenticated-orcid":false,"given":"Lark L.","family":"Coffey","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA"},{"name":"Department of Pathology, Microbiology &amp; Immunology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Jason","family":"Pitts","sequence":"additional","affiliation":[{"name":"Department of Biology, Baylor University, Waco, TX 76706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5139-739X","authenticated-orcid":false,"given":"Michaela","family":"Buenemann","sequence":"additional","affiliation":[{"name":"Department of Geography, New Mexico State University, Las Cruces, NM 88003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0708-3289","authenticated-orcid":false,"given":"Nikos","family":"Vasilakis","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Perera","sequence":"additional","affiliation":[{"name":"Institute of Health and Communiti Medicine, Universiti of Malaysia Sarawak, Sarawak 94300, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn A.","family":"Hanley","sequence":"additional","affiliation":[{"name":"Department of Biology, New Mexico State University, Las Cruces NM 88003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev-med-050715-105122","article-title":"Zika, Chikungunya, and other emerging vector-borne viral diseases","volume":"69","author":"Weaver","year":"2018","journal-title":"Annu. 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