{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:44:53Z","timestamp":1760233493989,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T00:00:00Z","timestamp":1611446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>In Mali, since 2007, artemether-lumefantrine has been the first choice against uncomplicated malaria. Despite its effectiveness, a rapid selection of markers of resistance to partner drugs has been documented. This work evaluated the treatment according to the World Health Organization\u2019s standard 28-day treatment method. The primary endpoint was the clinical and parasitological response corrected by a polymerase chain reaction. It was more than 99.9 percent, the proportion of patients with anemia significantly decrease compared to baseline (p &lt; 0.001), and no serious events were recorded. Plasmodium falciparum remains sensitive to artemether-lumefantrine in Mali.<\/jats:p>","DOI":"10.3390\/tropicalmed6010013","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T12:28:31Z","timestamp":1611577711000},"page":"13","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Monitoring of the Sensitivity In Vivo of Plasmodium falciparum to Artemether-Lumefantrine in Mali"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7866-9351","authenticated-orcid":false,"given":"Modibo","family":"Diarra","sequence":"first","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7412-0940","authenticated-orcid":false,"given":"Drissa","family":"Coulibaly","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amadou","family":"Tapily","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boureima","family":"Guindo","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koualy","family":"Sanogo","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diakalia","family":"Kon\u00e9","sequence":"additional","affiliation":[{"name":"National Malaria Control Program (PNLP-Mali), Bamako 223, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youssouf","family":"Kon\u00e9","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3251-5976","authenticated-orcid":false,"given":"Karim","family":"Kon\u00e9","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aboudramane","family":"Bathily","sequence":"additional","affiliation":[{"name":"Populations Services International-Mali (PSI-Mali), Bamako E5397, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oumar","family":"Yattara","sequence":"additional","affiliation":[{"name":"Populations Services International-Mali (PSI-Mali), Bamako E5397, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2679-035X","authenticated-orcid":false,"given":"Mahamadou A.","family":"Thera","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alassane","family":"Dicko","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0062-2283","authenticated-orcid":false,"given":"Abdoulaye A.","family":"Djimd\u00e9","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Pharmacy, University of Sciences Techniques and Technology of Bamako, Bamako BP 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J. Trop. Med. Hyg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1475-2875-8-34","article-title":"Efficacy of chloroquine, amodiaquine and sulphadoxine-pyrimethamine for the treatment of uncomplicated falciparum malaria: Revisiting molecular markers in an area of emerging AQ and SP resistance in Mali","volume":"8","author":"Tekete","year":"2009","journal-title":"Malar. J."},{"doi-asserted-by":"crossref","unstructured":"Reyburn, H. (2010). New WHO guidelines for the treatment of malaria. Br. Med. J. Publ. Group.","key":"ref_3","DOI":"10.1136\/bmj.c2637"},{"unstructured":"Boli, M. (2019). PNLP: Objectif, z\u00e9ro paludisme au Mali \u00e0 l\u2019horizon 2030. JSTM, Available online: https:\/\/www.jstm.org\/pnlp-objectif-zero-paludisme-au-mali-a-lhorizon-2030\/.","key":"ref_4"},{"unstructured":"WHO (2018). World Malaria Report 2018, World Health Organization. Available online: http:\/\/www.who.int\/malaria\/publications\/world-malaria-report-2018\/en\/.","key":"ref_5"},{"unstructured":"WHO (1996). Assessment of Therapeutic Efficacy of Antimalarial Drugs: For Uncomplicated Falciparum Malaria in Areas with Intense Transmission, World Health Organization. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/63295.","key":"ref_6"},{"unstructured":"(2020, November 19). Profile_mli_en.pdf. Available online: https:\/\/www.who.int\/malaria\/publications\/country-profiles\/profile_mli_en.pdf.","key":"ref_7"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.ijpara.2007.12.002","article-title":"Baseline in vitro efficacy of ACT component drugs on Plasmodium falciparum clinical isolates from Mali","volume":"38","author":"Kaddouri","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.4269\/ajtmh.14-0605","article-title":"Polymorphisms in the K13-Propeller Gene in Artemisinin-Susceptible Plasmodium falciparum Parasites from Bougoula-Hameau and Bandiagara, Mali","volume":"92","author":"Ouattara","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1475-2875-9-9","article-title":"Molecular markers of resistance to sulphadoxine-pyrimethamine one year after implementation of intermittent preventive treatment of malaria in infants in Mali","volume":"9","author":"Dicko","year":"2010","journal-title":"Malar. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"455","DOI":"10.4269\/ajtmh.2008.78.455","article-title":"Efficacy, safety, and selection of molecular markers of drug resistance by two ACTs in Mali","volume":"78","author":"Fofana","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1056\/NEJMoa0808859","article-title":"Artemisinin resistance in Plasmodium falciparum malaria","volume":"361","author":"Dondorp","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1056\/NEJMc0805011","article-title":"Evidence of artemisinin-resistant malaria in western Cambodia","volume":"359","author":"Noedl","year":"2008","journal-title":"N. Engl. J. Med."},{"unstructured":"WHO (2011). Global Plan for Artemisinin Resistance Containment\u2014GPARC (Archived), World Health Organization. Available online: http:\/\/www.who.int\/malaria\/publications\/atoz\/9789241500838\/en\/.","key":"ref_14"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1186\/1475-2875-10-339","article-title":"Standardizing the measurement of parasite clearance in falciparum malaria: The parasite clearance estimator","volume":"10","author":"Flegg","year":"2011","journal-title":"Malar. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.4269\/ajtmh.16-0234","article-title":"Plasmodium falciparum Resistance to Artemisinin Derivatives and Piperaquine: A Major Challenge for Malaria Elimination in Cambodia","volume":"95","author":"Duru","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/femsre\/fuw037","article-title":"The clinical impact of artemisinin resistance in Southeast Asia and the potential for future spread","volume":"41","author":"Woodrow","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00898-20","DOI":"10.1128\/AAC.00898-20","article-title":"Transmission of artemisinin-resistant malaria parasites to mosquitoes under antimalarial drug pressure","volume":"16","author":"Witmer","year":"2020","journal-title":"Antimicrob. Agents Chemother."},{"unstructured":"WHO (2008). Methods and Techniques for Clinical Trials on Antimalarial Drug Efficacy: Genotyping to Identify Parasite Populations, World Health Organization. Available online: http:\/\/www.who.int\/malaria\/publications\/atoz\/9789241596305\/en\/.","key":"ref_19"},{"doi-asserted-by":"crossref","unstructured":"World Health Organization, Communicable Diseases Cluster (2000). Severe falciparum malaria. Trans. R. Soc. Trop. Med. Hyg., 94, S1\u2013S90.","key":"ref_20","DOI":"10.1016\/S0035-9203(00)90300-6"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S1473-3099(15)00318-7","article-title":"Safety and efficacy of re-treatments with pyronaridine-artesunate in African patients with malaria: A substudy of the WANECAM randomised trial","volume":"16","author":"Sagara","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1111\/j.1365-3156.2006.01688.x","article-title":"Molecular genotyping to distinguish between recrudescents and new infections in treatment trials of Plasmodium falciparum malaria conducted in Sub-Saharan Africa: Adjustment of parasitological outcomes and assessment of genotyping effectiveness","volume":"11","author":"Mugittu","year":"2006","journal-title":"Trop. Med. Int. Health"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S0035-9203(97)90539-3","article-title":"Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon","volume":"91","author":"Taylor","year":"1997","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12936-018-2496-x","article-title":"A randomized trial of dihydroartemisinin\u2013piperaquine versus artemether\u2013lumefantrine for treatment of uncomplicated Plasmodium falciparum malaria in Mali","volume":"17","author":"Dama","year":"2018","journal-title":"Malar. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"634","DOI":"10.4269\/ajtmh.15-0503","article-title":"In Vivo Efficacy and Parasite Clearance of Artesunate + Sulfadoxine-Pyrimethamine Versus Artemether-Lumefantrine in Mali","volume":"94","author":"Dara","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"unstructured":"WHO (2009). Methods for Surveillance of Antimalarial Drug Efficacy, World Health Organization. Available online: http:\/\/www.who.int\/malaria\/publications\/atoz\/9789241597531\/en\/.","key":"ref_26"},{"unstructured":"Parasite Clearance Estimator (PCE) (2020, December 11). Worldwide Antimalarial Resistance Network. Available online: https:\/\/www.wwarn.org\/parasite-clearance-estimator-pce.","key":"ref_27"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1186\/1475-2875-13-309","article-title":"In vivo efficacy of artemether-lumefantrine and artesunate-amodiaquine for the treatment of uncomplicated falciparum malaria in children: A multisite, open-label, two-cohort, clinical trial in Mozambique","volume":"13","author":"Nhama","year":"2014","journal-title":"Malar. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1186\/s12936-017-1775-2","article-title":"Therapeutic efficacy and artemisinin resistance in northern Myanmar: Evidence from in vivo and molecular marker studies","volume":"16","author":"Myint","year":"2017","journal-title":"Malar. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/1475-2875-12-125","article-title":"Early parasitological response following artemisinin-containing regimens: A critical review of the literature","volume":"12","author":"Das","year":"2013","journal-title":"Malar. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1086\/650301","article-title":"In vivo parasitological measures of artemisinin susceptibility","volume":"201","author":"Stepniewska","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/1475-2875-7-185","article-title":"Distinction of Plasmodium falciparum recrudescence and re-infection by MSP2 genotyping: A caution about unstandardized classification criteria","volume":"7","author":"Rouse","year":"2008","journal-title":"Malar. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1006\/expr.1994.1070","article-title":"Plasmodium falciparum: Extensive polymorphism in merozoite surface antigen 2 alleles in an area with endemic malaria in Papua New Guinea","volume":"79","author":"Felger","year":"1994","journal-title":"Exp. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1093\/infdis\/jit431","article-title":"Residual Plasmodium falciparum Parasitemia in Kenyan Children After Artemisinin-Combination Therapy Is Associated With Increased Transmission to Mosquitoes and Parasite Recurrence","volume":"208","author":"Beshir","year":"2013","journal-title":"J. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1093\/infdis\/jit077","article-title":"Malaria Transmission After Artemether-Lumefantrine and Dihydroartemisinin-Piperaquine: A Randomized Trial","volume":"207","author":"Sawa","year":"2013","journal-title":"J. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-28179-2","article-title":"Identifying Recrudescent Plasmodium falciparum in Treated Malaria Patients by Real-time PCR and High Resolution Melt Analysis of Genetic Diversity","volume":"8","author":"Beshir","year":"2018","journal-title":"Sci. Rep."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/6\/1\/13\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:14:37Z","timestamp":1760159677000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/6\/1\/13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,24]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["tropicalmed6010013"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed6010013","relation":{},"ISSN":["2414-6366"],"issn-type":[{"type":"electronic","value":"2414-6366"}],"subject":[],"published":{"date-parts":[[2021,1,24]]}}}