{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:01:53Z","timestamp":1791291713489,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/202.658\/2018"],"award-info":[{"award-number":["E-26\/202.658\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/010.101076\/2018"],"award-info":[{"award-number":["E-26\/010.101076\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["301707\/2017-0"],"award-info":[{"award-number":["301707\/2017-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Zika virus (ZIKV) was recently introduced into the Western Hemisphere, where it is suspected to be transmitted mainly by Aedes aegypti in urban environments. ZIKV represents a public health problem as it has been implicated in congenital microcephaly in South America since 2015. Reports of ZIKV transmission in forested areas of Africa raises the possibility of its dispersal to non-human-modified environments in South America, where it is now endemic. The current study aimed to detect arboviruses in mosquitoes collected from areas with low human interference in Rio de Janeiro, Brazil. Using a sensitive pan-flavivirus RT-PCR, designed to detect the NS5 region, pools of Ae. albopictus and Haemagogus leucocelaenus, were positive for both ZIKV and yellow fever (YFV). Virus RNA was detected in pools of adult males and females reared from field-collected eggs. Findings presented here suggest natural vertical transmission and infection of ZIKV in Hg. leucocelaenus and Ae.albopitcus in Brazil.<\/jats:p>","DOI":"10.3390\/tropicalmed6020099","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T21:34:38Z","timestamp":1623360878000},"page":"99","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Natural Infection and Vertical Transmission of Zika Virus in Sylvatic Mosquitoes Aedes albopictus and Haemagogus leucocelaenus from Rio de Janeiro, Brazil"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7863-2698","authenticated-orcid":false,"given":"Jeronimo","family":"Alencar","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Diptera, Instituto Oswaldo Cruz (FIOCRUZ), Manguinhos 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9916-515X","authenticated-orcid":false,"given":"Cecilia","family":"Ferreira de Mello","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Diptera, Instituto Oswaldo Cruz (FIOCRUZ), Manguinhos 21040-360, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Serop\u00e9dica 23890-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Brisola Marcondes","sequence":"additional","affiliation":[{"name":"Departamento de Imunologia e Parasitologia de Microbiologia, Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-2471","authenticated-orcid":false,"given":"Anthony","family":"\u00c9rico Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Diptera, Instituto Oswaldo Cruz (FIOCRUZ), Manguinhos 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena Keiko","family":"Toma","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Molecular e Hematologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Queiroz Bastos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Diptera, Instituto Oswaldo Cruz (FIOCRUZ), Manguinhos 21040-360, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Serop\u00e9dica 23890-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shayenne","family":"Olsson Freitas Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Diptera, Instituto Oswaldo Cruz (FIOCRUZ), Manguinhos 21040-360, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Instituto Oswaldo Cruz (FIOCRUZ), Manguinhos 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Lisboa Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Molecular e Hematologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,11]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Hist\u00f3ria da Febre Amarela no Brasil","volume":"1","author":"Franco","year":"1969","journal-title":"Minist. 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