{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:23:32Z","timestamp":1790389412410,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Distinguished Research Professor Grant, Thailand Research Fund (TRF)","award":["DPG 6280002"],"award-info":[{"award-number":["DPG 6280002"]}]},{"name":"Distinguished Research Professor Grant, Thailand Research Fund (TRF)","award":["RP65-3-001"],"award-info":[{"award-number":["RP65-3-001"]}]},{"name":"Distinguished Research Professor Grant, Thailand Research Fund (TRF)","award":["PD2565-09"],"award-info":[{"award-number":["PD2565-09"]}]},{"name":"Distinguished Research Professor Grant, Thailand Research Fund (TRF)","award":["RO1CA164719"],"award-info":[{"award-number":["RO1CA164719"]}]},{"name":"Khon Kaen University, Research and Graduate Studies Affairs","award":["DPG 6280002"],"award-info":[{"award-number":["DPG 6280002"]}]},{"name":"Khon Kaen University, Research and Graduate Studies Affairs","award":["RP65-3-001"],"award-info":[{"award-number":["RP65-3-001"]}]},{"name":"Khon Kaen University, Research and Graduate Studies Affairs","award":["PD2565-09"],"award-info":[{"award-number":["PD2565-09"]}]},{"name":"Khon Kaen University, Research and Graduate Studies Affairs","award":["RO1CA164719"],"award-info":[{"award-number":["RO1CA164719"]}]},{"name":"Post-Doctoral Training Program from Khon Kaen University (KKU)","award":["DPG 6280002"],"award-info":[{"award-number":["DPG 6280002"]}]},{"name":"Post-Doctoral Training Program from Khon Kaen University (KKU)","award":["RP65-3-001"],"award-info":[{"award-number":["RP65-3-001"]}]},{"name":"Post-Doctoral Training Program from Khon Kaen University (KKU)","award":["PD2565-09"],"award-info":[{"award-number":["PD2565-09"]}]},{"name":"Post-Doctoral Training Program from Khon Kaen University (KKU)","award":["RO1CA164719"],"award-info":[{"award-number":["RO1CA164719"]}]},{"name":"National Cancer Institute, National Institutes of Health (NIH), USA","award":["DPG 6280002"],"award-info":[{"award-number":["DPG 6280002"]}]},{"name":"National Cancer Institute, National Institutes of Health (NIH), USA","award":["RP65-3-001"],"award-info":[{"award-number":["RP65-3-001"]}]},{"name":"National Cancer Institute, National Institutes of Health (NIH), USA","award":["PD2565-09"],"award-info":[{"award-number":["PD2565-09"]}]},{"name":"National Cancer Institute, National Institutes of Health (NIH), USA","award":["RO1CA164719"],"award-info":[{"award-number":["RO1CA164719"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Chronic human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support parasitological examination in diagnosing disease and screening for the risk of CCA. Here, we present an improved and innovative lateral flow immunochromatographic test (ICT) kit that uses whole-blood samples (WBS) rather than serum to diagnose human opisthorchiasis, which also successfully diagnosed human clonorchiasis. This ICT includes a soluble worm extract of O. viverrini adults and colloidal-gold-labeled conjugates of the IgG antibody to evaluate the diagnostic values with simulated WBS (n = 347). Simulated WBS were obtained by the spiking infection sera with red blood cells. The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy for detecting opisthorchiasis were 95.5%, 87.0%, 80.5%, 97.2%, and 90.1%, respectively. For clonorchiasis, these findings were 85.7%, 87.0%, 53.6%, 97.2%, and 86.8%, respectively. Combined for both diseases, they were 93.2%, 87.0%, 84.0%, 94.6%, and 89.6%, respectively. The ICT kit can possibly replace the ICT platforms for antibody detection in serum samples in field surveys in remote areas where sophisticated equipment is not available.<\/jats:p>","DOI":"10.3390\/tropicalmed7100308","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:31:01Z","timestamp":1666053061000},"page":"308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection"],"prefix":"10.3390","volume":"7","author":[{"given":"Lakkhana","family":"Sadaow","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutchanee","family":"Rodpai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penchom","family":"Janwan","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patcharaporn","family":"Boonroumkaew","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9411-8883","authenticated-orcid":false,"given":"Wannaporn","family":"Ittiprasert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, DC 20052, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria H.","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, DC 20052, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1765-0002","authenticated-orcid":false,"given":"Paul J.","family":"Brindley","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, DC 20052, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6726-3759","authenticated-orcid":false,"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2138-8783","authenticated-orcid":false,"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"ref_1","unstructured":"IARC Working Group (2012). 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