{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:23:34Z","timestamp":1760232214158,"version":"build-2065373602"},"reference-count":84,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Council of Thailand (NRCT) and the Thailand Science Research and Innovation (TSRI)","award":["PHD59\/0012"],"award-info":[{"award-number":["PHD59\/0012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Domestic and stray dogs can be frequently infected by Leptospira, and thus may represent a source for transmission of this zoonotic disease in Thailand. Here, we have used peptides derived from a recombinant leucine-rich repeat (LRR) protein of Leptospira, rKU_Sej_LRR_2012M, for the development of an indirect enzyme-linked immunosorbent assay (ELISA) aimed at detecting antibodies against Leptospira interrogans, L. borgpetersenii, and L. biflexa, the three major seroprevalences in Thai dogs. The rKU_Sej_LRR_2012M protein is recognized by hyperimmune sera against several leptospiral serovars. The epitope peptides of the rKU_Sej_LRR_2012M showed binding affinities with lower IC50 values than peptides of known antigenic protein LipL32. Four peptides, 2012-3T, 2012-4B, 2012-5B and pool 2012-B, were specifically recognized by rabbit hyperimmune sera against nine serovars from three Leptospira spp. The indirect peptide-based ELISAs with these four peptides were evaluated with the LipL32 ELISA by using a receiver\u2013operator curve (ROC) analysis. All peptides had an area under the curve of ROC (AUC) greater than 0.8, and the sum of sensitivity and specificity for each peptide was greater than 1.5. The degree of agreement of 2012-3T and pool 2012-B and 2012-4B and 2012-5B peptides were in moderate-to-good levels with kappa values of 0.41\u20130.60 and 0.61\u20130.80, when compared with LipL32, respectively. This finding would suggest an excellent capability of the 2012-4B and 2012-5B peptide-based ELISAs assay for the diagnosis of canine leptospiral infections.<\/jats:p>","DOI":"10.3390\/tropicalmed7100311","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:31:01Z","timestamp":1666053061000},"page":"311","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Leptospira borgpetersenii Leucine-Rich Repeat Proteins and Derived Peptides in an Indirect ELISA Development for the Diagnosis of Canine Leptospiral Infections"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0611-540X","authenticated-orcid":false,"given":"Sineenat","family":"Sripattanakul","sequence":"first","affiliation":[{"name":"The Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teerasak","family":"Prapong","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One-Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attapon","family":"Kamlangdee","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kamphaengsaen Campus, Nakorn Pathom 73140, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerd","family":"Katzenmeier","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One-Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8722-8176","authenticated-orcid":false,"given":"Dietmar","family":"Haltrich","sequence":"additional","affiliation":[{"name":"Department of Food Sciences and Technology, University of Natural Resources and Life Sciences,1180 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ratchanee","family":"Hongprayoon","sequence":"additional","affiliation":[{"name":"The Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8852-0286","authenticated-orcid":false,"given":"Siriwan","family":"Prapong","sequence":"additional","affiliation":[{"name":"The Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One-Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Samrot, A.V., Sean, T.C., Bhavya, K.S., Sahithya, C.S., Chan-Drasekaran, S., Palanisamy, R., Robinson, E.R., Subbiah, S.K., and Mok, P.L. 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