{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:56:03Z","timestamp":1777463763642,"version":"3.51.4"},"reference-count":53,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T00:00:00Z","timestamp":1666915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul (FAPERGS)","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Dogs are highly susceptible to leptospirosis and are a public health concern due to their important role as a source of spreading disease, particularly in urban settings. In this study, we present the pathogenesis, serological characterization, and complete genome sequencing of a virulent Brazilian strain (NEG7) of L. interrogans serovar Copenhageni isolated from the urine of a dog that died due to acute leptospirosis. Clinical investigation showed that the dog was presented with icteric mucous membranes, weakness, dehydration, anorexia, and kidney and liver failures. Necropsy followed by histopathological evaluation revealed lesions compatible with liver and kidney leptospirosis. The leptospires recovered from the urine were further characterized by genome analysis, which confirmed that the isolate belonged to L. interrogans serogroup icterohaemorrhagiae serovar Copenhageni. Multiple bioinformatics tools were used to characterize the genomic features, and comparisons with other available Copenhageni strains were performed. Characterization based on absence of an INDEL in the gene lic12008, associated with phylogenetic and ANI (99.99% identity) analyses, confirmed the genetic relatedness of the isolate with L. interrogans serovar Copenhageni. A better understanding of the diversity of the pathogenic Leptospira isolates could help in identifying genotypes responsible for severe infections. Moreover, it can be used to develop control and prevention strategies for Leptospira serovars associated with particular animal reservoirs.<\/jats:p>","DOI":"10.3390\/tropicalmed7110333","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T10:47:57Z","timestamp":1667126877000},"page":"333","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pathogenesis and Genomic Analysis of a Virulent Leptospira Interrogans Serovar Copenhageni Isolated from a Dog with Lethal Infection"],"prefix":"10.3390","volume":"7","author":[{"given":"Natasha Rodrigues","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Centro de Desenvolvimento Tecnol\u00f3gico, N\u00facleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederico Schmitt","family":"Kremer","sequence":"additional","affiliation":[{"name":"Centro de Desenvolvimento Tecnol\u00f3gico, N\u00facleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Risciela Salardi Alves","family":"de Brito","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0709-660X","authenticated-orcid":false,"given":"Rosimeri","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odir Ant\u00f4nio","family":"Dellagostin","sequence":"additional","affiliation":[{"name":"Centro de Desenvolvimento Tecnol\u00f3gico, N\u00facleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio","family":"Jorge","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.molimm.2018.11.005","article-title":"Immuno-Informatics Based Approaches to Design a Novel Multi Epitope-Based Vaccine for Immune Response Reinforcement against Leptospirosis","volume":"104","author":"Validi","year":"2018","journal-title":"Mol. 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