{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T13:28:25Z","timestamp":1793971705372,"version":"4.8.0"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz","award":["PJP INI-003-FIO-19-2-6"],"award-info":[{"award-number":["PJP INI-003-FIO-19-2-6"]}]},{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["PJP INI-003-FIO-19-2-6"],"award-info":[{"award-number":["PJP INI-003-FIO-19-2-6"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["PJP INI-003-FIO-19-2-6"],"award-info":[{"award-number":["PJP INI-003-FIO-19-2-6"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>New world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan Leishmania. Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of Leishmania spp. in animal tissues, yet it was not tested for the diagnosis of human NWCL. The aim of this study was to compare CISH, histopathology (HP), and immunohistochemistry (IHC) techniques to diagnose NWCL in human cutaneous lesions. The sample comprised fifty formalin-fixed, paraffin-embedded skin biopsy specimens from patients with NWCL caused by L. (V.) braziliensis. These specimens were analyzed by CISH, using a generic probe for Leishmania, IHC, and HP to assess the sensitivity of these methods by using a parasitological culture as a standard reference. Additional specimens from three patients diagnosed with cutaneous mycoses were also included to evaluate cross-reactions between CISH and IHC. The sensitivities of IHC, CISH, and HP for detecting amastigotes was 66%, 54%, and 50%, respectively. IHC, unlike CISH, cross-reacted with different species of fungi. Together, these results demonstrate that CISH may be a complementary assay for the detection of amastigote in the laboratorial diagnosis routine of human NWCL caused by L. (V.) braziliensis.<\/jats:p>","DOI":"10.3390\/tropicalmed7110344","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T03:44:17Z","timestamp":1667360657000},"page":"344","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples"],"prefix":"10.3390","volume":"7","author":[{"given":"Luiz Cl\u00e1udio","family":"Ferreira","sequence":"first","affiliation":[{"name":"Anatomic Pathology Service, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Pereira","family":"Quintella","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Service, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3460-1210","authenticated-orcid":false,"given":"Armando de Oliveira","family":"Schubach","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-1922","authenticated-orcid":false,"given":"Luciana de Freitas Campos","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria de F\u00e1tima","family":"Madeira","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria In\u00eas Fernandes","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rica de Camargo Ferreira e","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o T\u00e9cnico Educacional Souza Marques, Av. Ernani Cardoso, 335, Rio de Janeiro 21310-310, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Rosandiski","family":"Lyra","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel de Vasconcellos Carvalhaes de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Epidemiology, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1853-3449","authenticated-orcid":false,"given":"Rodrigo Caldas","family":"Menezes","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"key":"ref_1","first-page":"13","article-title":"Esp\u00e9cies neotropicais de Leishmania: Uma breve revis\u00e3o hist\u00f3rica sobre sua descoberta, ecologia e taxonomia","volume":"1","author":"Lainson","year":"2010","journal-title":"Rev. Panamazonica Saude."},{"key":"ref_2","unstructured":"Brazil Minist\u00e9rio da Sa\u00fade (2017). Manual de Vigil\u00e2ncia da Leishmaniose Tegumentar Americana."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/S0140-6736(18)31204-2","article-title":"Leishmaniasis","volume":"392","author":"Burza","year":"2018","journal-title":"Lancet"},{"key":"ref_4","unstructured":"Pan American Health Organization (2022, July 26). Leishmaniasis. 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