{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:30:39Z","timestamp":1790051439403,"version":"4.0.1"},"reference-count":64,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Public health officials around the world are extremely concerned about the global outbreak of monkeypox (MPX), which has been claimed to have originated in Africa. As a result, studies into the origins and reasons behind the outbreak\u2019s rapid spread have been sped up. The goal of the current investigation is to determine whether the monkeypox virus (MPXV) is present in seminal fluid samples from MPX cases that have been verified. Up until 6 January 2023, PubMed, Scopus, Web of Science, Embase, and ScienceDirect databases were used to conduct a thorough evaluation of the literature. The search technique returned a total of 308 items. Fourteen studies reporting the presence of MPXV in the seminal fluid of MPX-confirmed cases were included after the duplicates (n = 158) and searches by title, abstract, and full text were eliminated. In 84 out of the 643 confirmed MPX cases (13.06% or n = 643), MPXV was discovered in seminal fluid. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to identify MPXV, and samples taken from skin lesions (96.27%), pharynx or oropharynx (30.48%), and blood all had higher positivity rates than other samples (12.44%). Additionally, 99.85% of respondents were male with a mean age of 36, 98.45% engaged in MSM (men who have sex with men) sexual conduct, and human immunodeficiency virus (HIV) accounted for 56.9% of all STD cases. This study offers proof that MPXV can be found in the seminal fluid of MPX sufferers. Our data imply that MPXV transmission is a possibility in these samples and that MSM are more vulnerable to it. The creation of hygienic standards is essential for the early identification of MPX cases.<\/jats:p>","DOI":"10.3390\/tropicalmed8030173","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T04:39:45Z","timestamp":1678855185000},"page":"173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Virus Identification for Monkeypox in Human Seminal Fluid Samples: A Systematic Review"],"prefix":"10.3390","volume":"8","author":[{"given":"Joshuan J.","family":"Barboza","sequence":"first","affiliation":[{"name":"Escuela de Medicina, Universidad Cesar Vallejo, Trujillo 13007, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7267-0204","authenticated-orcid":false,"given":"Darwin A.","family":"Le\u00f3n-Figueroa","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Humana, Universidad de San Mart\u00edn de Porres, Chiclayo 15011, Peru"},{"name":"Centro de Investigaci\u00f3n en Atenci\u00f3n Primaria en Salud, Universidad Peruana Cayetano Heredia, Lima 15102, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hortencia M.","family":"Salda\u00f1a-Cumpa","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Humana, Universidad de San Mart\u00edn de Porres, Chiclayo 15011, Peru"},{"name":"Centro de Investigaci\u00f3n en Atenci\u00f3n Primaria en Salud, Universidad Peruana Cayetano Heredia, Lima 15102, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0839-2419","authenticated-orcid":false,"given":"Mario J.","family":"Valladares-Garrido","sequence":"additional","affiliation":[{"name":"South American Center for Education and Research in Public Health, Universidad Norbert Wiener, Lima 15108, Peru"},{"name":"Oficina de Epidemiolog\u00eda, Hospital Regional Lambayeque, Chiclayo 14012, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilly","family":"Moreno-Ramos","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Revisiones Sistem\u00e1ticas y Meta-An\u00e1lisis, Tau-Relaped Group, Trujillo 13007, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2695-8714","authenticated-orcid":false,"given":"Ranjit","family":"Sah","sequence":"additional","affiliation":[{"name":"Tribhuvan University Teaching Hospital, Institute of Medicine, Kathmandu 44600, Nepal"},{"name":"Department of Public Health Dentistry, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Pune 411018, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Katterine","family":"Bonilla-Aldana","sequence":"additional","affiliation":[{"name":"Research Unit, Universidad Continental, Huancayo 12000, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9773-2192","authenticated-orcid":false,"given":"Alfonso J.","family":"Rodriguez-Morales","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biomedicina, Faculty of Medicine, Fundaci\u00f3n Universitaria Aut\u00f3noma de las Am\u00e9ricas-Instituci\u00f3n Universitaria Visi\u00f3n de las Am\u00e9ricas, Pereira 660003, Colombia"},{"name":"Master of Clinical Epidemiology and Biostatistics, Universidad Cientifica del Sur, Lima 15067, Peru"},{"name":"Gilbert and Rose-Marie Chagoury School of Medicine, Lebanese American University, Beirut 1102, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5077","DOI":"10.1016\/j.vaccine.2020.04.062","article-title":"Human Monkeypox\u2013After 40 Years, an Unintended Consequence of Smallpox Eradication","volume":"38","author":"Simpson","year":"2020","journal-title":"Vaccine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e00092-22","DOI":"10.1128\/cmr.00092-22","article-title":"Monkeypox Virus Infections in Humans","volume":"35","author":"Elsayed","year":"2022","journal-title":"Clin. 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