{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T02:07:19Z","timestamp":1793326039687,"version":"4.5.0"},"reference-count":41,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Leptospirosis is a worldwide zoonotic disease. Pathogenic leptospires colonize the renal tubules and genital tract of animals and are excreted via urine. Transmission occurs via direct contact or through contaminated water or soil. The microscopic agglutination test (MAT) is the gold standard for the serodiagnosis of leptospirosis. The present study aims to evaluate animal exposure to Leptospira in the U.S. and Puerto Rico during the period 2018\u20132020. The presence of antibodies against pathogenic Leptospira spp. was assessed with the MAT according to the standards of the World Organisation for Animal Health. A total of 568 sera were submitted for diagnostic, surveillance, or import\/export testing from the U.S. and Puerto Rico. Seropositivity (\u22651:100) was 51.8% (294\/568) with agglutinating antibodies found in 115 (39.1%) cattle, 84 (28.6%) exotic animals, 38 (12.9%) horses, 22 (7.5%) goats, 15 (5.1%) dogs, 11 (3.7%) swine, and 9 (3.1%) sheep. The most detected serogroups were Australis, Grippotyphosa, and Ballum. The results showed that animals were exposed to serogroups\/serovars not included in commercial bacterins such as Ballum, Bratislava (only in swine vaccine), and Tarassovi. Our findings suggest that more studies should include culture and concomitant genotyping to reduce animal disease and zoonotic risk through efficacious vaccine and diagnostic strategies.<\/jats:p>","DOI":"10.3390\/tropicalmed8030183","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T08:36:16Z","timestamp":1679474176000},"page":"183","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Animals Exposed to Leptospira Serogroups Not Included in Bacterins in the United States and Puerto Rico"],"prefix":"10.3390","volume":"8","author":[{"given":"Tammy","family":"Anderson","sequence":"first","affiliation":[{"name":"National Veterinary Services Laboratories, APHIS, U.S. Department of Agriculture, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Hamond","sequence":"additional","affiliation":[{"name":"National Veterinary Services Laboratories, APHIS, U.S. Department of Agriculture, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a","family":"Haluch","sequence":"additional","affiliation":[{"name":"National Veterinary Services Laboratories, APHIS, U.S. Department of Agriculture, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kari","family":"Toot","sequence":"additional","affiliation":[{"name":"National Veterinary Services Laboratories, APHIS, U.S. Department of Agriculture, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9478-1316","authenticated-orcid":false,"given":"Jarlath E.","family":"Nally","sequence":"additional","affiliation":[{"name":"Infectious Bacterial Diseases Research Unit, ARS, U.S. Department of Agriculture, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9455-3380","authenticated-orcid":false,"given":"Karen","family":"LeCount","sequence":"additional","affiliation":[{"name":"National Veterinary Services Laboratories, APHIS, U.S. Department of Agriculture, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda K.","family":"Schlater","sequence":"additional","affiliation":[{"name":"National Veterinary Services Laboratories, APHIS, U.S. Department of Agriculture, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Costa, F., Wunder, E.A., De Oliveira, D., Bisht, V., Rodrigues, G., Reis, M.G., Ko, A.I., Begon, M., and Childs, J.E. 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