{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:56:46Z","timestamp":1791997006863,"version":"4.4.0"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Higher Education, Malaysia","award":["LRGS\/1\/2018\/UM\/01\/1\/4 (LR002D-2018)"],"award-info":[{"award-number":["LRGS\/1\/2018\/UM\/01\/1\/4 (LR002D-2018)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>We propose a protocol suitable for point-of-care diagnosis of malaria utilizing a simple and purification-free DNA extraction method with the combination of loop-mediated isothermal amplification assay and lateral flow (LAMP-LF). The multiplex LAMP-LF platform developed here can simultaneously detect Plasmodium knowlesi, P. vivax, P. falciparum, and Plasmodium genus (for P. malariae and P. ovale). Through the capillary effect, the results can be observed by the red band signal on the test and control lines within 5 min. The developed multiplex LAMP-LF was tested with 86 clinical blood samples on-site at Hospital Kapit, Sarawak, Malaysia. By using microscopy as the reference method, the multiplex LAMP-LF showed 100% sensitivity (95% confidence interval (CI): 91.4 to 100.00%) and 97.8% specificity (95% CI: 88.2% to 99.9%). The high sensitivity and specificity of multiplex LAMP-LF make it ideal for use as a point-of-care diagnostic tool. The simple and purification-free DNA extraction protocol can be employed as an alternative DNA extraction method for malaria diagnosis in resource-limited settings. By combining the simple DNA extraction protocol and multiplex LAMP-LF approach, we aim to develop a simple-to-handle and easy-to-read molecular diagnostic tool for malaria in both laboratory and on-site settings.<\/jats:p>","DOI":"10.3390\/tropicalmed8040199","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T01:05:26Z","timestamp":1680138326000},"page":"199","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Point-of-Care Diagnosis of Malaria Using a Simple, Purification-Free DNA Extraction Method Coupled with Loop-Mediated Isothermal Amplification-Lateral Flow"],"prefix":"10.3390","volume":"8","author":[{"given":"Meng Yee","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Phone Youth","family":"Zen","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Hafizi","family":"Abdul Hamid","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Sector, Ministry of Health, Putrajaya 62590, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenarun","family":"Jelip","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Sector, Ministry of Health, Putrajaya 62590, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rose Nani","family":"Mudin","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Sector, Ministry of Health, Putrajaya 62590, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vun Jan Shui","family":"Ivan","sequence":"additional","affiliation":[{"name":"Hospital Kapit, Pathology Unit, Jalan Mamora, Kapit 96800, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Ngie Ping","family":"Francis","sequence":"additional","affiliation":[{"name":"Hospital Kapit, Pathology Unit, Jalan Mamora, Kapit 96800, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izreena","family":"Saihidi","sequence":"additional","affiliation":[{"name":"Hospital Kapit, Pathology Unit, Jalan Mamora, Kapit 96800, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2021). World Malaria Report 2021, World Health Organization."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1373\/clinchem.2015.245829","article-title":"Recombinase polymerase amplification for diagnostic applications","volume":"62","author":"Daher","year":"2016","journal-title":"Clin. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"700","DOI":"10.4269\/ajtmh.17-0738","article-title":"Recombinase polymerase amplification combined with a lateral flow strip for the detection of Plasmodium knowlesi","volume":"98","author":"Lai","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e01879-19","DOI":"10.1128\/JCM.01879-19","article-title":"Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections","volume":"58","author":"Lalremruata","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.jmoldx.2013.05.005","article-title":"Detection and species identification of malaria parasites by isothermal tHDA amplification directly from human blood without sample preparation","volume":"15","author":"Li","year":"2013","journal-title":"J. Mol. Diagn."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"103570","DOI":"10.1016\/j.fm.2020.103570","article-title":"Comparative study of multiplex real-time recombinase polymerase amplification and ISO11290-1 methods for the detection of Listeria monocytogenes in dairy products","volume":"92","author":"Azinheiro","year":"2020","journal-title":"Food Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1021\/acs.analchem.5b03267","article-title":"A multiplexed recombinase polymerase amplification assay to detect intestinal protozoa","volume":"88","author":"Crannell","year":"2016","journal-title":"Anal. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1128\/JCM.43.1.402-405.2005","article-title":"Real-time nucleic acid sequence-based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum","volume":"43","author":"Schneider","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.trac.2019.01.015","article-title":"LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification","volume":"113","author":"Zhang","year":"2019","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"961832","DOI":"10.3389\/fcimb.2022.961832","article-title":"Rapid diagnosis of Plasmodium falciparum malaria using a point-of-care loop-mediated isothermal amplification device","volume":"12","author":"Puri","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"167","DOI":"10.2144\/000113072","article-title":"Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue","volume":"46","author":"Goto","year":"2009","journal-title":"BioTechniques."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2144\/000114253","article-title":"Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes","volume":"58","author":"Tanner","year":"2015","journal-title":"BioTechniques"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s11274-019-2601-5","article-title":"Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae","volume":"35","author":"Zhang","year":"2019","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1051\/parasite\/2021039","article-title":"A novel loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) device for rapid detection of Toxoplasma gondii in the blood of stray cats and dogs","volume":"28","author":"Xue","year":"2021","journal-title":"Parasite."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.vetpar.2016.02.004","article-title":"Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"219","author":"Yang","year":"2016","journal-title":"Vet. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.diagmicrobio.2010.08.026","article-title":"Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick","volume":"69","author":"Njiru","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.3389\/fmicb.2019.02135","article-title":"Development and clinical validation of multiple cross displacement amplification combined with nanoparticles-based biosensor for detection of Mycobacterium tuberculosis: Preliminary results","volume":"10","author":"Jiao","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.2147\/IDR.S211644","article-title":"Lateral flow biosensor combined with loop-mediated isothermal amplification for simple, rapid, sensitive, and reliable detection of Brucella spp.","volume":"12","author":"Li","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e2003916","DOI":"10.1371\/journal.pbio.2003916","article-title":"Nucleic acid purification from plants, animals and microbes in under 30 seconds","volume":"15","author":"Zou","year":"2017","journal-title":"PloS Biol."},{"key":"ref_21","first-page":"2","article-title":"Assured-compliant point-of-care diagnostics for the detection of human viral infections","volume":"32","author":"Naseri","year":"2021","journal-title":"Rev. Med. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Otoo, J.A., and Schlappi, T.S. (2022). REASSURED Multiplex Diagnostics: A critical review and forecast. Biosensors, 12.","DOI":"10.3390\/bios12020124"},{"key":"ref_23","unstructured":"Kettler, H., White, K., and Hawkes, S. (2021, February 10). Mapping the Landscape of Diagnostics for Sexually Transmitted Infections: Key Findings and Recommendations. World Health Organization. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/68990."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0166-6851(93)90077-B","article-title":"High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction","volume":"61","author":"Snounou","year":"1993","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1128\/JCM.00811-09","article-title":"Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi","volume":"47","author":"Imwong","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jbi.2014.02.013","article-title":"Sample size estimation in diagnostic test studies of biomedical informatics","volume":"48","year":"2014","journal-title":"J. Biomed. Inform."},{"key":"ref_27","unstructured":"Hermanson, G.T. (2008). Bioconjugate Techniques, Academic Press."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/978-1-4939-6352-2_4","article-title":"Preparation of colloidal gold particles and conjugation to protein A\/g\/L, IGG, F(AB\u2032)2, and streptavidin","volume":"1476","author":"Yokota","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"336","DOI":"10.4269\/ajtmh.15-0569","article-title":"Loop-mediated isothermal amplification assay for identification of five human Plasmodium species in Malaysia","volume":"94","author":"Lau","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/CMR.00079-12","article-title":"Human infections and detection of Plasmodium knowlesi","volume":"26","author":"Singh","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Sharma, S., Kumar, S., Ahmed, M.Z., Bhardwaj, N., Singh, J., Kumari, S., Savargaonkar, D., Anvikar, A.R., and Das, J. (2021). Advanced multiplex loop-mediated isothermal amplification (mLAMP) combined with lateral flow detection (LFD) for rapid detection of two prevalent malaria species in India and melting curve analysis. Diagnostics, 12.","DOI":"10.3390\/diagnostics12010032"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.parint.2014.06.004","article-title":"Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax","volume":"63","author":"Yongkiettrakul","year":"2014","journal-title":"Parasitol. Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"704","DOI":"10.4269\/ajtmh.18-0177","article-title":"Development of loop-mediated isothermal amplification-based lateral flow device method for the detection of malaria","volume":"99","author":"Mallepaddi","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/8\/4\/199\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:06:08Z","timestamp":1760123168000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/8\/4\/199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,29]]},"references-count":33,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["tropicalmed8040199"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed8040199","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,29]]}}}