{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T02:47:34Z","timestamp":1794019654881,"version":"4.8.0"},"reference-count":65,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordination of Superior Level Staff Improvement (CAPES)","award":["88887.659754\/2021-00"],"award-info":[{"award-number":["88887.659754\/2021-00"]}]},{"name":"Coordination of Superior Level Staff Improvement (CAPES)","award":["013\/2021-UEPA"],"award-info":[{"award-number":["013\/2021-UEPA"]}]},{"name":"Graduate Program in Parasitary Biology in the Amazon Region (PPGBPA) of State University of Par\u00e1","award":["88887.659754\/2021-00"],"award-info":[{"award-number":["88887.659754\/2021-00"]}]},{"name":"Graduate Program in Parasitary Biology in the Amazon Region (PPGBPA) of State University of Par\u00e1","award":["013\/2021-UEPA"],"award-info":[{"award-number":["013\/2021-UEPA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>West Nile virus is characterized as a neurotropic pathogen, which can cause West Nile fever and is transmitted by mosquitoes of the genus Culex. In 2018, the Instituto Evandro Chagas performed the first isolation of a WNV strain in Brazil from a horse brain sample. The present study aimed to evaluate the susceptibility of orally infected Cx. quinquefasciatus from the Amazon region of Brazil to become infected and transmit the WNV strain isolated in 2018. Oral infection was performed with blood meal artificially infected with WNV, followed by analysis of infection, dissemination, and transmission rates, as well as viral titers of body, head, and saliva samples. At the 21st dpi, the infection rate was 100%, the dissemination rate was 80%, and the transmission rate was 77%. These results indicate that Cx. quinquefasciatus is susceptible to oral infection by the Brazilian strain of WNV and may act as a possible vector of the virus since it was detected in saliva from the 21st dpi.<\/jats:p>","DOI":"10.3390\/tropicalmed8040217","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T02:29:58Z","timestamp":1680748198000},"page":"217","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Vector Competence of Culex quinquefasciatus from Brazil for West Nile Virus"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3522-3584","authenticated-orcid":false,"given":"L\u00facia Aline Moura","family":"Reis","sequence":"first","affiliation":[{"name":"Graduate Program in Parasitary Biology in the Amazon Region, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana Vieira Pinto da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute\u2014IEC\/MS\/SVSA, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Damous","family":"Dias","sequence":"additional","affiliation":[{"name":"Graduate Program in Parasitary Biology in the Amazon Region, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-9649","authenticated-orcid":false,"given":"Maria Nazar\u00e9 Oliveira","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute\u2014IEC\/MS\/SVSA, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6843-0822","authenticated-orcid":false,"given":"Rossela Damasceno","family":"Caldeira","sequence":"additional","affiliation":[{"name":"Graduate Program in Biology of Infectious and Parasitary Agents, Biological Sciences Institute, Federal University of Par\u00e1, Bel\u00e9m 66077-830, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7199-9304","authenticated-orcid":false,"given":"Pedro Arthur da Silva","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Graduate Program in Biology of Infectious and Parasitary Agents, Biological Sciences Institute, Federal University of Par\u00e1, Bel\u00e9m 66077-830, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Silva da","family":"Silva","sequence":"additional","affiliation":[{"name":"Graduate Program in Parasitary Biology in the Amazon Region, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Wilson","family":"Rosa Junior","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute\u2014IEC\/MS\/SVSA, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Carlos Feitosa","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute\u2014IEC\/MS\/SVSA, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8529-4751","authenticated-orcid":false,"given":"Bruna La\u00eds Sena do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas 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