{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:13:10Z","timestamp":1792789990867,"version":"4.5.0"},"reference-count":76,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T00:00:00Z","timestamp":1681948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"VALE S.A."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated the circulation of the virus since 2003, with the first human case detected in 2014. The objective of the present paper is to report the first isolation of WNV in a Culex (Melanoconion) mosquito. Arthropods were collected by protected human attraction and CDC light bait, and taxonomically identified and analyzed by viral isolation, complement fixation and genomic sequencing tests. WNV was isolated from samples of Culex (Melanoconion) mosquitoes, and the sequencing analysis demonstrated that the isolated strain belonged to lineage 1a. The finding of the present study presents the first evidence of the isolation and genome sequencing of WNV in arthropods in Brazil.<\/jats:p>","DOI":"10.3390\/tropicalmed8040237","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T01:33:43Z","timestamp":1682040823000},"page":"237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["First Isolation and Genome Sequence Analysis of West Nile Virus in Mosquitoes in Brazil"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5981-0065","authenticated-orcid":false,"given":"Joaquim Pinto Nunes","family":"Neto","sequence":"first","affiliation":[{"name":"Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3522-3584","authenticated-orcid":false,"given":"L\u00facia Aline Moura","family":"Reis","sequence":"additional","affiliation":[{"name":"Graduate Program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Nazar\u00e9 Oliveira","family":"Freitas","sequence":"additional","affiliation":[{"name":"Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8529-4751","authenticated-orcid":false,"given":"Bruna La\u00eds Sena","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane Leal","family":"das Chagas","sequence":"additional","affiliation":[{"name":"Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8972-749X","authenticated-orcid":false,"given":"Hernan Hermes Monteiro","family":"da Costa","sequence":"additional","affiliation":[{"name":"Immunology Center, Adolfo Lutz Institute, S\u00e3o Paulo 01246-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica Cec\u00edlia Pinheiro","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory Animal Husbandry and Production Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Margalho","family":"Braga","sequence":"additional","affiliation":[{"name":"Graduate Program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana Vieira Pinto","family":"da Silva","sequence":"additional","affiliation":[{"name":"Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7185-4538","authenticated-orcid":false,"given":"Sandro Patroca","family":"Silva","sequence":"additional","affiliation":[{"name":"Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9400-2036","authenticated-orcid":false,"given":"L\u00edvia Caricio","family":"Martins","sequence":"additional","affiliation":[{"name":"Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1128\/CMR.00045-12","article-title":"West Nile Virus: Biology, Transmission, and Human Infection","volume":"25","author":"Colpitts","year":"2012","journal-title":"Clin. 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