{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:57:18Z","timestamp":1790985438840,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPESP","award":["2019\/17488-2"],"award-info":[{"award-number":["2019\/17488-2"]}]},{"name":"FAPESP","award":["2018\/08131-0"],"award-info":[{"award-number":["2018\/08131-0"]}]},{"name":"FAPESP","award":["2016\/11541-0"],"award-info":[{"award-number":["2016\/11541-0"]}]},{"name":"FAPESP","award":["2017\/06731-8"],"award-info":[{"award-number":["2017\/06731-8"]}]},{"name":"FAPESP","award":["301229\/2017-1"],"award-info":[{"award-number":["301229\/2017-1"]}]},{"name":"FAPESP","award":["304445\/2021-51"],"award-info":[{"award-number":["304445\/2021-51"]}]},{"name":"CNPq","award":["2019\/17488-2"],"award-info":[{"award-number":["2019\/17488-2"]}]},{"name":"CNPq","award":["2018\/08131-0"],"award-info":[{"award-number":["2018\/08131-0"]}]},{"name":"CNPq","award":["2016\/11541-0"],"award-info":[{"award-number":["2016\/11541-0"]}]},{"name":"CNPq","award":["2017\/06731-8"],"award-info":[{"award-number":["2017\/06731-8"]}]},{"name":"CNPq","award":["301229\/2017-1"],"award-info":[{"award-number":["301229\/2017-1"]}]},{"name":"CNPq","award":["304445\/2021-51"],"award-info":[{"award-number":["304445\/2021-51"]}]},{"name":"Fundacao Butantan","award":["2019\/17488-2"],"award-info":[{"award-number":["2019\/17488-2"]}]},{"name":"Fundacao Butantan","award":["2018\/08131-0"],"award-info":[{"award-number":["2018\/08131-0"]}]},{"name":"Fundacao Butantan","award":["2016\/11541-0"],"award-info":[{"award-number":["2016\/11541-0"]}]},{"name":"Fundacao Butantan","award":["2017\/06731-8"],"award-info":[{"award-number":["2017\/06731-8"]}]},{"name":"Fundacao Butantan","award":["301229\/2017-1"],"award-info":[{"award-number":["301229\/2017-1"]}]},{"name":"Fundacao Butantan","award":["304445\/2021-51"],"award-info":[{"award-number":["304445\/2021-51"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Pathogenic leptospires can bind to receptors on mammalian cells such as cadherins and integrins. Leptospira effectively adheres to cells, overcomes host barriers and spreads into the bloodstream, reaching internal target organs such as the lungs, liver and kidneys. Several microorganisms produce proteins that act as ligands of integrins through the RGD motif. Here, we characterized a leptospiral RGD-containing protein encoded by the gene lic12254. In silico analysis of pathogenic, intermediate and saprophytic species showed that LIC12254 is highly conserved among pathogenic species, and is unique in presenting the RGD motif. The LIC12254-coding sequence is greatly expressed in the virulent Leptospira interrogans L1-130 strain compared with the culture-attenuated L. interrogans M20 strain. We also showed that the recombinant protein rLIC12254 binds to \u03b1V\u03b28 and \u03b18 human integrins most likely via the RGD motif. These interactions are dose-dependent and saturable, a typical property of receptor\u2013ligand interactions. The binding of the recombinant protein lacking this motif\u2014rLIC12254 \u0394RAA\u2014to \u03b1V\u03b28 was almost totally abolished, while that with the \u03b18 human integrin was decreased by 65%. Taken together, these results suggest that this putative outer membrane protein interacts with integrins via the RGD domain and may play a key role in leptospirosis pathogenesis.<\/jats:p>","DOI":"10.3390\/tropicalmed8050249","type":"journal-article","created":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T01:28:28Z","timestamp":1682558908000},"page":"249","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["LIC12254 Is a Leptospiral Protein That Interacts with Integrins via the RGD Motif"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4277-2805","authenticated-orcid":false,"given":"Maria F.","family":"Cavenague","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, S\u00e3o Paulo 05503-000, SP, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1171-8312","authenticated-orcid":false,"given":"Aline F.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, S\u00e3o Paulo 05503-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G. V.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, S\u00e3o Paulo 05503-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4851-0870","authenticated-orcid":false,"given":"Ana L. T. O.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, S\u00e3o Paulo 05503-000, SP, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Costa, F., Hagan, J.E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M.S., Stein, C., Abela-Ridder, B., and Ko, A.I. (2015). Global Morbidity and Mortality of Leptospirosis: A Systematic Review. PLoS Negl. Trop. 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